Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,281,208 |
A→G |
L525L (CTA→CTG) |
yejF → |
fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,281,208 | 0 | A | G | 100.0%
| 182.9
/ NA
| 49 | L525L (CTA→CTG) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/32); total (17/32) |
AAGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTACTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATT > W3110S.gb/2281153‑2281263
|
aaGGAGCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg < 1:1082292/65‑1 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGtt > 1:1101293/1‑64 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg < 1:2102874/65‑1 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg > 1:2323749/1‑65 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg < 1:493843/65‑1 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg < 1:1456832/65‑1 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg > 1:785085/1‑65 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg > 1:845966/1‑65 (MQ=255)
aaGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACCCGTCAGCTGCTGGCGTTg < 1:38684/65‑1 (MQ=255)
aGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTg < 1:1604460/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:705132/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:946168/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:2046821/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:1948045/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:2482598/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:409419/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:518954/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:53289/64‑1 (MQ=255)
acacCGCAGCAGGAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAg < 1:1382421/64‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:396645/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:588294/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:822838/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:654836/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:2325892/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:682774/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:2054589/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1020173/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:2006446/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1137696/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1201531/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:126478/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1293513/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1408459/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1607554/65‑1 (MQ=255)
gAGTATACGCGTCAGCTGCTGGCATTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1458694/65‑1 (MQ=255)
aGTATACGCGTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGtt < 1:1796438/64‑1 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1615757/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:982011/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:120144/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:125423/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1483281/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2084534/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:474670/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1619695/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:1758695/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:362223/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2411020/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAtt > 1:2266025/1‑64 (MQ=255)
cgTCAGCTGCTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAAt > 1:1855176/1‑63 (MQ=255)
|
AAGGACCGTGCGCGCGCGTGTTTGCCACACCGCAGCAGGAGTATACGCGTCAGCTACTGGCGTTGAGCTGACGCTTAAAAAGGATTGTAGTTTGAAAAGGGTTCGGCAATT > W3110S.gb/2281153‑2281263
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
