Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,620,788 |
A→G |
E312E (GAA→GAG) |
purM → |
phosphoribosylaminoimidazole synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,620,788 | 0 | A | G | 100.0%
| 16.7
/ NA
| 6 | E312E (GAA→GAG) | purM | phosphoribosylaminoimidazole synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (2/4); total (2/4) |
GCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAA > W3110S.gb/2620754‑2620840
|
gcgTCGGGATGATTATTGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGcc > 1:2990935/1‑65 (MQ=255)
tgatTATTGCCCTGCCTGCTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGaa > 1:1528847/1‑65 (MQ=255)
gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa < 1:1789691/65‑1 (MQ=255)
gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa < 1:616261/65‑1 (MQ=255)
gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa < 1:6229/65‑1 (MQ=255)
gcctgcTCCGGAGGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGaaaa < 1:734595/65‑1 (MQ=255)
|
GCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAA > W3110S.gb/2620754‑2620840
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
