Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,960,928 |
T→C |
K53E (AAG→GAG) |
recC ← |
exonuclease V (RecBCD complex), gamma chain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,960,928 | 0 | T | C | 100.0%
| 132.2
/ NA
| 36 | K53E (AAG→GAG) | recC | exonuclease V (RecBCD complex), gamma chain |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/9); total (27/9) |
GCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTTTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATCTCT > W3110S.gb/2960884‑2960989
|
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:104681/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:906547/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:739889/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:662357/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:417288/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:413069/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:354076/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:352224/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:350521/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:3003206/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:2800923/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:2786603/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:2486990/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:2398700/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:1736129/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:1039910/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:1365289/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:1573261/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:1870778/1‑65 (MQ=255)
gCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCa > 1:2272959/1‑65 (MQ=255)
tGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACt < 1:2841948/65‑1 (MQ=255)
aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:1562492/64‑1 (MQ=255)
aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:2119193/64‑1 (MQ=255)
aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:1369553/64‑1 (MQ=255)
aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:1404322/64‑1 (MQ=255)
aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:1555927/64‑1 (MQ=255)
aGCGGAAAATCAATGTTTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:2591026/64‑1 (MQ=255)
aGCGGAAAATCAATGTCTGCCGCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACtgtg < 1:3095469/64‑1 (MQ=255)
gCAATACCAAACTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAg < 1:2531425/65‑1 (MQ=255)
aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc > 1:2033013/1‑65 (MQ=255)
aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc > 1:52501/1‑65 (MQ=255)
aaCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCAtctc > 1:1884562/1‑65 (MQ=255)
aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct > 1:234876/1‑65 (MQ=255)
aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct > 1:625094/1‑65 (MQ=255)
aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct > 1:714698/1‑65 (MQ=255)
aCTCTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATctct > 1:2975248/1‑65 (MQ=255)
|
GCTCGCTGGCAGCGGAAAATCAATGTTTGCCGCAATACCAAACTTTTGCGACAGGGTCATTTGCAGCCACTGTGCCATACCGGTACTTTGCACCAGAATCATCTCT > W3110S.gb/2960884‑2960989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
