Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,036,331 |
(C)5→6 |
coding (432/1734 nt) |
recJ ← |
ssDNA exonuclease, 5' ‑‑> 3'‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,036,326 | 1 | . | C | 100.0%
| 27.1
/ NA
| 9 | V146G (GTT→GGT) | recJ | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/9); total (0/9) |
CCCAACGAGCGAGCGTGCTCAA‑CCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACA > W3110S.gb/3036305‑3036364
|
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:1183381/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:1558294/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:2720250/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:2760919/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:2866757/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:513702/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:828324/61‑1 (MQ=255)
cccAACGAGCGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACAGTGACa < 1:1175843/61‑1 (MQ=255)
cccAACGAGAGAGCGTGCTCAACCCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACa < 1:910074/61‑1 (MQ=255)
|
CCCAACGAGCGAGCGTGCTCAA‑CCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACA > W3110S.gb/3036305‑3036364
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
