Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,291,041 |
A→G |
L791L (CTA→CTG) |
yraJ → |
predicted outer membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,291,041 | 0 | A | G | 100.0%
| 71.0
/ NA
| 20 | L791L (CTA→CTG) | yraJ | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/20); total (0/20) |
ACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCG > W3110S.gb/3290995‑3291059
|
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:2762417/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:946207/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:745963/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:526556/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:373675/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:366976/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:365618/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:293527/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:2838796/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:1199719/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:262384/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:2234496/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:1650062/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:1380267/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:1333361/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:12218/65‑1 (MQ=255)
aCGATGATACGGGGCAACAAAGTATCGTCGATGAGGACGGCATACTGTATCTCTCTGGGATATCg < 1:2693219/65‑1 (MQ=255)
cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:2658937/64‑1 (MQ=255)
cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:1523896/64‑1 (MQ=255)
cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCg < 1:525329/64‑1 (MQ=255)
|
ACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCG > W3110S.gb/3290995‑3291059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
