Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
507,425 |
C→A |
16.3% |
A71D (GCT→GAT) |
ybhH → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 507,425 | 0 | C | A | 16.3%
| 188.8
/ 14.2
| 67 | A71D (GCT→GAT) | ybhH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (36/20); new base A (3/8); total (39/28) |
| Fisher's exact test for biased strand distribution p-value = 4.17e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01 |
ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTG > minE/507356‑507491
|
aCGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:594563/71‑1 (MQ=255)
gTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:1444749/1‑70 (MQ=255)
ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTc > 1:1484277/1‑71 (MQ=255)
ttGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTc > 1:1094162/1‑71 (MQ=255)
ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:1643407/1‑71 (MQ=255)
ggcggcGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGa > 1:668829/1‑71 (MQ=255)
ggTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCt < 1:530867/71‑1 (MQ=255)
gTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTg < 1:904493/71‑1 (MQ=255)
tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt < 1:479779/71‑1 (MQ=255)
tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt < 1:600602/71‑1 (MQ=255)
tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGt < 1:1187740/71‑1 (MQ=255)
tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGt < 1:2025606/71‑1 (MQ=255)
tAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGt < 1:2146481/71‑1 (MQ=255)
aTCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:1681516/1‑55 (MQ=255)
aTCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGa > 1:22913/1‑55 (MQ=255)
tCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTg < 1:684670/71‑1 (MQ=255)
tCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTg < 1:529354/71‑1 (MQ=255)
cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGtt > 1:1015053/1‑67 (MQ=255)
cACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTgcg > 1:12881/1‑71 (MQ=255)
gACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAg > 1:403515/1‑70 (MQ=255)
ccAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCt > 1:1866028/1‑58 (MQ=255)
cAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCt < 1:213584/43‑1 (MQ=255)
aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:1419817/1‑71 (MQ=255)
aaaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGt > 1:309958/1‑71 (MQ=255)
aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:420205/1‑51 (MQ=255)
aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:1643796/1‑51 (MQ=255)
aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:1936139/1‑51 (MQ=255)
aaGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATc > 1:771432/1‑51 (MQ=255)
aaGTCGCCATTATTAGCCGTTACAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCg > 1:63483/1‑69 (MQ=255)
tCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt < 1:1628770/71‑1 (MQ=255)
cGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:1362622/1‑70 (MQ=255)
gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAgg > 1:198633/1‑58 (MQ=255)
gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:520425/1‑69 (MQ=255)
gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCAt > 1:848173/1‑69 (MQ=255)
gCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa < 1:39850/71‑1 (MQ=255)
attattAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAg > 1:313720/1‑69 (MQ=255)
attattAGCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGc > 1:301351/1‑70 (MQ=255)
ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa < 1:2022894/71‑1 (MQ=255)
ttattaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCaa > 1:477524/1‑71 (MQ=255)
attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGttt > 1:1223329/1‑45 (MQ=255)
attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGttt > 1:1243218/1‑45 (MQ=255)
attaGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc < 1:1616689/70‑1 (MQ=255)
aGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:1250102/1‑62 (MQ=255)
gCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:1084232/71‑1 (MQ=255)
gCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTc < 1:1031246/71‑1 (MQ=255)
gCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACgtgt < 1:469591/70‑1 (MQ=255)
gCCGTTCCAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACgtgt < 1:997419/70‑1 (MQ=255)
ccGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAg < 1:362347/61‑1 (MQ=255)
cGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGa < 1:1171179/71‑1 (MQ=255)
gTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGAt < 1:2118205/71‑1 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGa > 1:1012831/1‑55 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATa > 1:380399/1‑69 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1425501/1‑70 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:813307/1‑70 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:395138/1‑70 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc > 1:1700392/1‑70 (MQ=255)
ccAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc < 1:388201/70‑1 (MQ=255)
ccAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc < 1:1331863/70‑1 (MQ=255)
ccAGCGATCCGCGTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATAc < 1:1198880/70‑1 (MQ=255)
cAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAAc < 1:755013/59‑1 (MQ=255)
aGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAg > 1:2110944/1‑54 (MQ=255)
gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:1997233/1‑71 (MQ=255)
gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg < 1:480219/71‑1 (MQ=255)
gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:1115571/1‑71 (MQ=255)
gCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACg > 1:1106843/1‑71 (MQ=255)
tCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTa < 1:680770/71‑1 (MQ=255)
cgTGATGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTg > 1:1720346/1‑71 (MQ=255)
|
ACGGTATTGGCGGCGGTAATCCACTGACCAGTAAAGTCGCCATTATTAGCCGTTCCAGCGATCCGCGTGCTGATGTCGATTATCTGTTTGCGCAGGTAATCGTTCATGAGCAACGTGTCGATACCACGCCTAACTG > minE/507356‑507491
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
