Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,869,135 T→G T207T (ACA→ACC)  ygcW ← predicted deoxygluconate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,869,1350TG88.1% 668.5 / 53.0 210T207T (ACA→ACC) ygcWpredicted deoxygluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/0);  new base G (185/0);  total (210/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

GAGTATCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACC  >  minE/1869068‑1869203
                                                                   |                                                                    
gAGTATTACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:684067/1‑71 (MQ=255)
gAGTATGACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:200979/1‑71 (MQ=255)
gAGTATCACGCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1077183/1‑71 (MQ=255)
gAGTATCACCCCAACTGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:696730/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:1380844/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:1412910/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:1311590/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:607519/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:673943/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:711990/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:223938/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:1066161/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACgggt                                                                    >  1:16503/1‑70 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:38640/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:567599/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:468103/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:450760/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:43589/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:23501/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:396608/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:393477/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:255759/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:281171/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:37200/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:361546/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:286375/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:352176/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:325936/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:289501/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:319431/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:697640/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1003289/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:955641/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:922081/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:908309/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:881396/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:867601/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:827549/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:783604/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:760715/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:706066/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:493094/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:678707/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:67804/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:632339/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:581957/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:568768/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:973919/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:542823/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:527593/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:506403/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1146360/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1324772/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:233618/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:129578/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1279014/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1275645/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1237790/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1214599/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1203511/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1186640/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:115551/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:132059/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1130168/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1114049/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1104488/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1095869/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1073230/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1061054/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1047208/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1043234/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1030189/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1024621/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1493530/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:209832/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:192168/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:186543/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:182811/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:172969/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1555558/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1536627/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1501391/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1329786/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1489036/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1473262/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1466416/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1443542/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1426896/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1402172/1‑71 (MQ=255)
gAGTATCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:1359834/1‑71 (MQ=255)
gAGTACCACCCCAACGGTTTGCCGGAATATGATCAAGAACACGCTGGTTAGTTTCTGGATTACTACGGGTc                                                                   >  1:897164/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACGTGAATATTATATTGAcc  >  1:1429744/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:1450928/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:732647/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:330343/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:1227500/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:419004/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:884684/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGAcc  >  1:239849/1‑71 (MQ=255)
                                                                 cGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCACTTACCTGAATATTATATTGAcc  >  1:1182862/1‑71 (MQ=255)
                                                                   |                                                                    
GAGTATCGCCCCAACGGTTTGCCGGAATATGATCAAGAACGCGCTGATTGGTTTCTGGATTACTGCGTGTCGCCAGCGTAATATCTGTTGCATAATAGCCAGGGGCGATACCATTTACCTGAATATTATATTGACC  >  minE/1869068‑1869203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A