Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I3 R1 176 0.0 1562086 58.2% 909134 66.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,956,670 +C coding (1009/1233 nt) serA ← D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,956,6671.C100.0% 523.8 / NA 144R338G (CGT→GGT) serAD‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (144/0);  total (144/0)

Only 100 of 144 total aligned reads displayed.

AGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACG‑CCCACCGTGCAGTGGCAGCGAGACTTCCGG  >  minE/1956627‑1956697
                                         |                              
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCCCCGTGCAGTGGCAGCGAGACTTCCg   >  1:1525995/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAg              >  1:211146/1‑60 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGc             >  1:1281958/1‑61 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGCCTTCCg   >  1:1480058/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTccc   >  1:126043/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:1409506/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:618661/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:223679/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:1382289/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:873368/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:89499/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTcc    >  1:1455475/1‑70 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:706169/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:61072/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:671626/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:646730/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:643776/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:673425/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:697138/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:412330/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:593325/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:586806/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:585665/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:572510/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:55458/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:491741/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:483106/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:444706/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:433716/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:426594/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:418623/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:350705/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:84896/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:984945/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:960792/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:938086/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:937141/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:907687/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:901963/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:888284/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:887482/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:874383/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:865462/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:857400/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:712514/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:844938/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:825891/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:806304/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:785888/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:768066/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:764670/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:741226/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:739378/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:72074/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:718035/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1195423/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1501678/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1443926/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1431753/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1375781/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1322888/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:132068/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1275386/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:12744/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1252353/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1228109/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1221340/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1512659/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1175325/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1156633/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1151002/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1140130/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1111593/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1110395/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1095843/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1070773/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1022284/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1002452/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:292381/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:386979/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:361915/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:360180/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1001880/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:332985/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:329980/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:311676/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:300156/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:293493/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:400508/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:277568/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:272272/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:251394/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:190759/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:17867/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:159046/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:1548782/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:152209/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGAGCCAGCGAGACTTCCg   >  1:255565/1‑71 (MQ=255)
aGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCCACAGTGCAGTGGCAGCGAGACTTCCg   >  1:1559572/1‑71 (MQ=255)
aGCACGCCCGGAAGGTTTTCGTGGATGTGCATCAGACGACGCCCCACCGTGCAGTGGCAGCGAGACTTCCg   >  1:306199/1‑71 (MQ=255)
                                         |                              
AGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACG‑CCCACCGTGCAGTGGCAGCGAGACTTCCGG  >  minE/1956627‑1956697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A