Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R2 146 74.7 3299319 84.8% 2797822 66.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,302,228 C→T V285M (GTG→ATG)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,302,2280CT100.0% 348.6 / NA 103V285M (GTG→ATG) dacDD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/58);  total (45/58)

Only 100 of 103 total aligned reads displayed.

CCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCA  >  minE/1302181‑1302292
                                               |                                                                
ccAGATGCGTTCCGTTGCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:1757617/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGttt                                                     >  1:863201/1‑61 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2794699/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:1651097/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:3105716/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2207725/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:1405387/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2344488/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2848419/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:333018/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:1959519/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:1295790/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:756047/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2377784/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:863849/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:869157/1‑70 (MQ=255)
ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2113557/1‑70 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:3225108/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:492989/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:557114/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:1561449/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:1555703/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:603207/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:1348259/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:2381388/70‑1 (MQ=255)
                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCtt                   >  1:1860780/1‑71 (MQ=255)
                             cACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    <  1:1527657/65‑1 (MQ=255)
                                 gTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc          <  1:1668650/71‑1 (MQ=255)
                                 gTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc          <  1:589465/71‑1 (MQ=255)
                                 gTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc          <  1:446581/71‑1 (MQ=255)
                                 gTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc          <  1:356030/71‑1 (MQ=255)
                                 gTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc          <  1:72117/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:2302045/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:288815/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:876058/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:3228015/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:2000083/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:1980844/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:490981/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:1671329/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:1442145/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:665523/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:688341/71‑1 (MQ=255)
                                       aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:1057332/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:490888/70‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:835381/70‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:2622376/70‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:2727152/70‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:1377781/70‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:260304/70‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:2609177/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:1473721/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:1418344/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:646111/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:1346148/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:1902956/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3283430/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3241082/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:210085/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:1212324/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:2269607/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3089197/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:2703993/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3040996/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3025455/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:2664635/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3025433/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:2556765/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:1102190/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:231278/71‑1 (MQ=255)
                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3051577/71‑1 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                    >  1:606144/1‑53 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCct                      >  1:1949536/1‑51 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa              >  1:293679/1‑59 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGCCCTTTTGCa  >  1:412138/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg    <  1:2021210/69‑1 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:738952/70‑1 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1175258/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1077062/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:388246/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1358306/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:854879/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:828574/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1199836/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1607360/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:556840/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1730996/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1806230/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:457766/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1886797/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:408271/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3184418/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3111604/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3075266/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:2864770/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:2350913/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:2497353/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1032635/1‑71 (MQ=255)
                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCGCTTCCTCACGACCTTTTGc   <  1:1646192/70‑1 (MQ=255)
                                         attGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1048731/2‑71 (MQ=255)
                                               |                                                                
CCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCA  >  minE/1302181‑1302292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A