Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R1
|
28 |
4.9 |
280516 |
80.6% |
226095 |
64.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
558,918 |
T→C |
P55P (CCT→CCC) |
cmr → |
multidrug efflux system protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 558,918 | 0 | T | C | 100.0%
| 12.1
/ NA
| 5 | P55P (CCT→CCC) | cmr | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (4/1); total (4/1) |
TGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGG > minE/558857‑558943
|
tGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGAt > 1:12102/1‑70 (MQ=255)
tGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGAt > 1:139543/1‑70 (MQ=255)
gTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGATGACCGCGTATCTg > 1:59648/1‑71 (MQ=255)
tGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGAt < 1:172940/55‑1 (MQ=255)
ggtggAACAATATCAGGCGGGCATTGATTGGGTTCCCACTTCGATGACCGCGTATCTggcgg > 1:53550/1‑62 (MQ=255)
|
TGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGG > minE/558857‑558943
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
