Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R1
|
28 |
4.9 |
280516 |
80.6% |
226095 |
64.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,845,199 |
G→T |
P266T (CCG→ACG) |
cysD ← |
sulfate adenylyltransferase, subunit 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,845,199 | 0 | G | T | 100.0%
| 14.1
/ NA
| 6 | P266T (CCG→ACG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (4/2); total (4/2) |
ATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGA > minE/1845141‑1845258
|
aTCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAgtgt < 1:205566/65‑1 (MQ=255)
aCGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcaccg > 1:121333/1‑71 (MQ=255)
aGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCagcagc > 1:99597/1‑70 (MQ=255)
ctctTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCg > 1:105191/1‑71 (MQ=255)
ctctTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCg > 1:142825/1‑71 (MQ=255)
gatgatTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGa < 1:189820/71‑1 (MQ=255)
|
ATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGA > minE/1845141‑1845258
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
