Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R1
|
28 |
4.9 |
280516 |
80.6% |
226095 |
64.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,864,584 |
C→T |
A295A (GCC→GCT) |
hflK → |
modulator for HflB protease specific for phage lambda cII repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,864,584 | 0 | C | T | 100.0%
| 14.0
/ NA
| 6 | A295A (GCC→GCT) | hflK | modulator for HflB protease specific for phage lambda cII repressor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (2/4); total (2/4) |
GAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATA > minE/2864519‑2864643
|
gAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGAc > 1:187765/1‑71 (MQ=255)
cGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAg < 1:183639/71‑1 (MQ=255)
cgcAACGTATCCTCGAAGAGGAGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAg < 1:181408/59‑1 (MQ=255)
tATCCTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCAt > 1:231329/1‑39 (MQ=255)
cTCGAAGAGGCGCGTGCGTACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTa < 1:254270/70‑1 (MQ=255)
cgtACAAGGCTCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAata < 1:120809/70‑1 (MQ=255)
|
GAAGTTCAGCCGCGTGCGAACGGTCAGGCGCAACGTATCCTCGAAGAGGCGCGTGCGTACAAGGCCCAGACCATCCTGGAAGCTCAGGGTGAAGTGGCGCGCTTTGCTAAACTTCTGCCGGAATA > minE/2864519‑2864643
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
