Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,455 |
Δ1 bp |
100% |
coding (614/1647 nt) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,455 | 0 | G | . | 100.0%
| 93.2
/ NA
| 22 | coding (614/1647 nt) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (0/22); total (0/22) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTC > minE/1015429‑1015493
|
catccAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:2281367/61‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:289757/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:989847/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:877185/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:820341/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:756979/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:705614/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:558756/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:515466/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:327076/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:298269/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:290294/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:273996/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:2300153/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:2037516/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1751387/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:151278/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1253038/64‑1 (MQ=255)
cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1208725/64‑1 (MQ=255)
cAACCAGGTAA‑TTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1110156/63‑1 (MQ=255)
tAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1996657/56‑1 (MQ=255)
aTTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1545434/54‑1 (MQ=255)
|
CAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTC > minE/1015429‑1015493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
