Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,875,977 |
A→G |
100% |
N262S (AAC→AGC) |
ygcG → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,875,977 | 0 | A | G | 78.3%
| 40.4
/ 7.7
| 23 | N262S (AAC→AGC) | ygcG | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base G (0/18); total (5/18) |
| Fisher's exact test for biased strand distribution p-value = 2.97e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGC > minE/1875912‑1875989
|
tATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaac > 1:1437501/1‑67 (MQ=255)
tATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaac > 1:616098/1‑67 (MQ=255)
cTTTACCTGGTTATGTGTGCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1788942/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAATAAGCTTCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1553180/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:788810/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:765318/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:586740/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:384727/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:380666/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:289973/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2242060/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2135429/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2133107/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:2103686/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1920379/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1747079/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1712766/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:166825/66‑1 (MQ=255)
cTTTACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:1486280/66‑1 (MQ=255)
tttACCTGGTTATGTGTCCCACAGAAAAAAGCATCAGGTAGAAGTTATCATTCAGACAGcggcgg < 1:223426/65‑1 (MQ=255)
ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:479772/1‑67 (MQ=255)
ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:481823/1‑67 (MQ=255)
ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:484432/1‑67 (MQ=255)
ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:1473017/1‑67 (MQ=255)
ccTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGc > 1:1239255/1‑67 (MQ=255)
|
TATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGC > minE/1875912‑1875989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
