Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,914,848 |
T→C |
100% |
E208E (GAA→GAG) |
ptsP ← |
fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,914,848 | 0 | T | C | 100.0%
| 29.0
/ NA
| 10 | E208E (GAA→GAG) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/0); total (10/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAG > minE/1914799‑1914865
|
ggctAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:841514/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGCGCCGGATCCAg > 1:305327/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1139690/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1214613/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:144285/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1790556/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:1838893/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:2099121/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:692802/1‑67 (MQ=255)
ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAg > 1:770470/1‑67 (MQ=255)
|
GGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAG > minE/1914799‑1914865
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
