Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F3 I0 R1	328	90.1	2317887	85.2%	1974839	62.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,081,439	C→T	100%	A378A (GCC→GCT)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,081,439	0	C	T	80.4%	228.9 / 46.0	107	A378A (GCC→GCT)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base C (21/0); new base T (86/0); total (107/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 107 total aligned reads displayed.

CGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGT  >  minE/2081382‑2081483

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGc                                      >  1:2084992/1‑66 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:1724826/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:2255102/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:2269642/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:2202029/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:2058489/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:204849/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:540084/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:541769/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:558246/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:187600/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:695483/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:158140/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:825188/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:830778/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:1483669/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:1452743/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCa                                     >  1:961596/1‑67 (MQ=255)

cGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATAGGTGGTTTTGCCCACCAGGCa                                     >  1:233850/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCCCCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1700851/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCGGGCACTGTCTGGCGCACTGATTACGctctct          >  1:1408962/1‑59 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTg                                  >  1:325611/1‑35 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGgcgcac                        >  1:1129084/1‑43 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGGACTGATTACGCTCTCtt         >  1:927928/1‑60 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGacg   >  1:1099041/1‑64 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:337622/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:943874/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:500675/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:482606/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:464526/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:462089/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:435278/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:9618/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:991934/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:32988/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:307956/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:235273/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:981500/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:683570/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:851785/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:858037/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:889464/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:896228/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:758355/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:727990/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:924712/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:68677/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:930644/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2137688/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:666923/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:649942/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:614043/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:580382/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:579146/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:565868/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:925590/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1376191/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1742562/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1692865/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1651219/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1649713/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1635846/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1609237/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1559986/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1557182/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1467803/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1386078/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2246840/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1370185/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1369111/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1337029/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1321485/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1310902/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1296357/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1138988/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1082929/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1074545/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:104698/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1941327/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2257736/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2183826/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2166221/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1045293/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2127012/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2097964/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2051027/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2044227/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:2008815/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1966542/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1930946/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1889975/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1884159/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1881569/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1857821/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1811991/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1780981/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:1778162/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTCACCAGGCACTGTCTGGCGCACTGATTACGCTCTATTCTGAcgt  >  1:1856741/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTAACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:663963/1‑67 (MQ=255)

                                   tgctgTGTATCGGTGGTTTTGCTAACCAGGCACTGTCTGGCGCACTGATTACGCTCTCTTCTGAcgt  >  1:523098/1‑67 (MQ=255)

CGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGT  >  minE/2081382‑2081483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A