Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,262,618 |
C→T |
100% |
intergenic (‑227/+186) |
rplJ ← / ← rplA |
50S ribosomal subunit protein L10/50S ribosomal subunit protein L1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,262,618 | 0 | C | T | 100.0%
| 68.9
/ NA
| 21 | intergenic (‑227/+186) | rplJ/rplA | 50S ribosomal subunit protein L10/50S ribosomal subunit protein L1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/0); total (21/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTT > minE/2262579‑2262645
|
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAg > 1:1721471/1‑53 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1941693/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:955944/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:903541/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:649392/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:584488/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:464818/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:329500/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:283883/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:2201791/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:2090661/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:2043656/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1013475/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1858631/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1653505/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1515999/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:148914/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1419865/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:141265/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGtt > 1:1031542/1‑67 (MQ=255)
ccTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATTCGTCGCTATTCAGCTTGTGACGGCGt > 1:1020168/1‑66 (MQ=255)
|
CCTGGATAGGCCAGGCTCCAACGAACAAATCTTTTCTATCCGTCGCTATTCAGCTTGTGACGGCGTT > minE/2262579‑2262645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
