Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R1
|
328 |
90.1 |
2317887 |
85.2% |
1974839 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,642,512 |
C→T |
100% |
G48G (GGC→GGT) |
gntR → |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,642,512 | 0 | C | T | 100.0%
| 173.1
/ NA
| 50 | G48G (GGC→GGT) | gntR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (50/0); total (50/0) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTTGATGAACTGGGCTATATTCCCAATCGTGCGC > minE/2642466‑2642531
|
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATcgcgcg > 1:753494/1‑65 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATcgcgcg > 1:1483845/1‑65 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:382852/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:190089/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1974843/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2113466/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2168033/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2180230/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2183661/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2271635/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2278742/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:2292770/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:321572/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:37888/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:379771/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:18802/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:608509/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:638020/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:660442/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:685742/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:731930/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:767879/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:818019/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:850108/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:866515/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:911485/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1536359/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1099934/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1127695/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1197746/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1236378/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1246600/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:132991/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1354184/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1355112/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1369818/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1416822/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1446362/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1079665/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1558086/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1589541/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1636076/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1656006/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1667689/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1680762/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1739354/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1778576/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:186106/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1862403/1‑66 (MQ=255)
ccGTCGCTCTACGCGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCgcgcgc > 1:1863458/1‑66 (MQ=255)
|
CCGTCGCTCTACGCGGCAAGATTGCCGCGGCTCTTGATGAACTGGGCTATATTCCCAATCGTGCGC > minE/2642466‑2642531
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
