Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,552,318 |
T→C |
F67S (TTT→TCT) |
murP → |
fused predicted enzyme IIBC components of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,552,318 | 0 | T | C | 97.4%
| 135.0
/ ‑4.8
| 39 | F67S (TTT→TCT) | murP | fused predicted enzyme IIBC components of PTS |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (20/18); minor base A (1/0); total (21/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 |
CCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAG > W3110S.gb/2552252‑2552385
|
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:976181/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:734299/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:1636548/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:1759355/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:1771355/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:1811453/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:3340116/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:2209478/70‑1 (MQ=255)
ccAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTgg < 1:2984386/70‑1 (MQ=255)
tcatCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCt < 1:1529622/69‑1 (MQ=255)
tcatCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCt < 1:3819069/69‑1 (MQ=255)
tcatCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCt < 1:102441/69‑1 (MQ=255)
catCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCt < 1:502819/69‑1 (MQ=255)
atCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCt < 1:300084/69‑1 (MQ=255)
tCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg < 1:1312826/68‑1 (MQ=255)
tCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg < 1:2424215/68‑1 (MQ=255)
tCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg < 1:2363747/68‑1 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:2514096/1‑64 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:736735/1‑64 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:142628/1‑64 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:3988719/1‑64 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:3568384/1‑64 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:213393/1‑64 (MQ=255)
aTTTTGACCAGCGACCAGGTGCAGGTCGTTTATGGGCCTGGTAAAGCGCATCGTGCCGCaaaa > 1:3668490/1‑63 (MQ=255)
ttGACCAGCGCCCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCa > 1:481646/1‑47 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCaaaa > 1:2439251/1‑60 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCaaaa > 1:2587079/1‑60 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCaaaa > 1:612277/1‑60 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCaaa > 1:2535733/1‑59 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagc > 1:2113868/1‑69 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagc > 1:2149705/1‑69 (MQ=255)
ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagc > 1:670527/1‑69 (MQ=255)
tGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg < 1:3630831/62‑1 (MQ=255)
cGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAg > 1:634722/1‑38 (MQ=255)
cGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc > 1:957519/1‑39 (MQ=255)
cGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc > 1:2455933/1‑39 (MQ=255)
cGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc > 1:3292290/1‑39 (MQ=255)
cGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTa > 1:3699367/1‑70 (MQ=255)
cGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTa > 1:256380/1‑70 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:3921406/71‑1 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:732332/71‑1 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:3040059/71‑1 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:2857640/71‑1 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:917205/71‑1 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:2790784/71‑1 (MQ=255)
tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:963093/71‑1 (MQ=255)
|
CCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAG > W3110S.gb/2552252‑2552385
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
