breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 45,813 C→T P3S (CCG→TCG)  yaaU → predicted transporter
RA 52,852 G→A H189Y (CAT→TAT)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
RA 53,012 A→G G135G (GGT→GGC pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
RA 65,135 C→T D216N (GAT→AAT)  polB ← DNA polymerase II
RA 70,244 T→C intergenic (‑196/‑143) araB ← / → araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
RA 155,463 A→T *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 230,040 C→A R281L (CGC→CTC)  yafC ← predicted DNA‑binding transcriptional regulator
RA 234,234 C→T L183L (TTG→TTA gloB ← predicted hydroxyacylglutathione hydrolase
RA 259,305 G→A A7V (GCA→GTA)  phoE ← outer membrane phosphoporin protein E
RA 344,381 G→A intergenic (+166/‑247) yahL → / → yahM hypothetical protein/hypothetical protein
RA 381,851 A→C G88G (GGT→GGG yaiF ← hypothetical protein
RA 393,168 T→C G310G (GGT→GGC yaiU → hypothetical protein
RA 442,006 G→A D412N (GAT→AAT)  thiI → sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis
RA 480,905 C→T G908E (GGG→GAG)  acrB ← multidrug efflux system protein
RA 515,934 C→T A43V (GCG→GTG)  ybbM → predicted inner membrane protein
RA 547,694 A→G pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,835 +G pseudogene (255/261 nt)
pseudogene (255/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 579,680 G→A intergenic (+371/‑377) ybcW → / → nohB hypothetical protein/DNA packaging protein
RA 644,304 C→T intergenic (‑78/+36) rna ← / ← citT ribonuclease I/citrate:succinate antiporter
RA 644,447 C→T G453S (GGC→AGC)  citT ← citrate:succinate antiporter
MC JC 654,214 Δ1,199 bp [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 742,687 T→A I98F (ATC→TTC)  ybgH ← predicted transporter
RA 810,589 T→G M275R (ATG→AGG)  bioB → biotin synthase
RA 827,597 C→T P26P (CCG→CCA ybhS ← predicted transporter subunit
RA 859,070 G→A Y138Y (TAC→TAT ybiU ← hypothetical protein
RA 904,996 G→A intergenic (‑107/‑19) ybjP ← / → ybjQ predicted lipoprotein/conserved hypothetical protein
RA 906,962 G→A H72Y (CAC→TAC)  ybjS ← predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 913,779 A→G L153P (CTG→CCG)  hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 927,929 C→T Q563Q (CAG→CAA cydC ← fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily
RA 941,935 C→T T185T (ACC→ACT dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A
RA 959,125 A→G T351A (ACA→GCA)  serC → 3‑phosphoserine/phosphohydroxythreonine aminotransferase
RA 1,016,291 T→C V52A (GTA→GCA)  rmf → ribosome modulation factor
RA 1,020,867 A→G S159P (TCC→CCC)  sulA ← SOS cell division inhibitor
RA 1,056,565 G→A G12G (GGC→GGT torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,109,964 G→A C185C (TGC→TGT mdoC ← membrane protein required for modification of periplasmic glucan
RA 1,144,552 G→A R465C (CGT→TGT)  rne ← fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome
RA 1,172,268 T→C K1092E (AAG→GAG)  mfd ← transcription‑repair coupling factor
RA 1,173,170 C→T R791H (CGT→CAT)  mfd ← transcription‑repair coupling factor
RA 1,175,236 C→A Q102H (CAG→CAT mfd ← transcription‑repair coupling factor
RA 1,181,288 T→G D64E (GAT→GAG cobB → deacetylase of acetyl‑CoA synthetase, NAD‑dependent
RA 1,244,320 C→T C113Y (TGC→TAC)  ldcA ← L,D‑carboxypeptidase A
MC JC 1,301,041 Δ1,336 bp insCinsD insC, insD
RA 1,303,631 C→T Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
RA 1,339,708 G→A A722T (GCC→ACC)  acnA → aconitate hydratase 1
RA 1,357,653 G→A P391L (CCC→CTC)  sapA ← predicted antimicrobial peptide transporter subunit
RA 1,373,368 G→A R480H (CGT→CAT)  ycjM → predicted glucosyltransferase
RA 1,376,428 G→A V209M (GTG→ATG)  ycjP → predicted sugar transporter subunit
RA 1,396,058 A→G Y373C (TAT→TGT)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,401,010 T→C K77K (AAA→AAG fnr ← DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth
RA 1,463,502 C→T P299L (CCG→CTG)  paaJ → predicted beta‑ketoadipyl CoA thiolase
RA 1,477,935 G→A E360K (GAA→AAA)  ydbD → hypothetical protein
RA 1,521,063 C→T S108F (TCC→TTC)  yncB → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
RA 1,522,458 G→A R161R (AGG→AGA yncC → predicted DNA‑binding transcriptional regulator
RA 1,569,965 T→C Q191Q (CAA→CAG yddW ← predicted liprotein
RA 1,588,010 T→G D64A (GAC→GCC)  ydeP ← predicted oxidoreductase
RA 1,595,970 (C)8→9 coding (3831/3978 nt) ydeK ← predicted lipoprotein
RA 1,620,710 C→T intergenic (‑88/‑124) marC ← / → marR predicted transporter/DNA‑binding transcriptional repressor
RA 1,657,562 T→C I14T (ATT→ACT)  ynfB → hypothetical protein
RA 1,692,839 G→A G425D (GGT→GAT)  ydgA → conserved hypothetical protein
RA 1,742,822 A→G S400G (AGC→GGC)  ydhC → predicted transporter
RA 1,753,336 T→C T35A (ACG→GCG)  ydhW ← hypothetical protein
RA 1,761,776 C→T L154L (CTG→CTA sufS ← selenocysteine lyase, PLP‑dependent
RA 1,775,080 T→C F285F (TTT→TTC ydiN → predicted transporter
RA 1,846,641 (A)8→7 intergenic (‑56/+72) ynjI ← / ← topB predicted inner membrane protein/DNA topoisomerase III
RA 1,887,874 T→C F106L (TTT→CTT)  yeaX → predicted oxidoreductase
RA 1,927,287 T→C L45P (CTT→CCT)  yobB → conserved hypothetical protein
RA 1,965,064 G→A I567I (ATC→ATT flhA ← predicted flagellar export pore protein
RA 1,995,272 T→C S524S (TCA→TCG uvrC ← excinuclease UvrABC, endonuclease subunit
RA 2,006,018 A→G I4V (ATT→GTT)  fliD → flagellar filament capping protein
RA 2,035,876 (T)8→9 intergenic (‑255/‑312) yedR ← / → yedS predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment
RA 2,077,980 C→T Y355Y (TAC→TAT yeeR → predicted membrane protein
RA 2,092,365 A→G K13E (AAA→GAA)  hisG → ATP phosphoribosyltransferase
RA 2,157,782 T→C L128S (TTG→TCG)  mdtB → multidrug efflux system, subunit B
RA 2,160,543 C→T F7F (TTC→TTT mdtC → multidrug efflux system, subunit C
RA 2,169,967 (T)7→8 intergenic (‑10/+159) yegZ ← / ← yegR ECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein
MC JC 2,176,983 Δ1,199 bp [gatA][gatA] [gatA], insH, [gatA]
RA 2,198,825 G→A A398T (GCG→ACG)  metG → methionyl‑tRNA synthetase
RA 2,233,079 G→A A214V (GCG→GTG)  dusC ← tRNA‑dihydrouridine synthase C
RA 2,258,347 C→T E58E (GAG→GAA rihB ← ribonucleoside hydrolase 2
RA 2,276,693 Δ1 bp coding (996/1815 nt) yejA → predicted oligopeptide transporter subunit
RA 2,298,699 (T)5→6 coding (16/480 nt) ccmE ← periplasmic heme chaperone
RA 2,313,823 T→C E349G (GAA→GGA)  yojL ← predicted thiamine biosynthesis lipoprotein
RA 2,315,568 A→G G172G (GGT→GGC ompC ← outer membrane porin protein C
MC JC 2,320,795 Δ1,336 bp insD[rcsC] insD, insC, [rcsC]
RA 2,325,916 A→G N402D (AAT→GAT)  atoS → sensory histidine kinase in two‑component regulatory system with AtoC
RA 2,350,543 G→A V337I (GTA→ATA)  nrdA → ribonucleoside diphosphate reductase 1, alpha subunit
RA 2,381,932 Δ1 bp coding (331/759 nt) yfbB ← predicted peptidase
RA 2,397,191 A→G F174S (TTC→TCC)  nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 2,427,890 T→G I246S (ATT→AGT)  yfcH → conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
RA 2,453,614 A→G A91A (GCT→GCC prmB ← N5‑glutamine methyltransferase
RA 2,488,439 T→A K116I (AAA→ATA)  emrK ← EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
RA 2,501,495 G→A S135S (TCG→TCA ddg → palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
RA 2,512,691 (G)5→4 coding (650/1248 nt) ypdG ← predicted enzyme IIC component of PTS
RA 2,541,664 G→A E129K (GAA→AAA)  crr → glucose‑specific enzyme IIA component of PTS
RA 2,552,318 T→C F67S (TTT→TCT)  murP → fused predicted enzyme IIBC components of PTS
RA 2,589,664 A→T intergenic (+300/‑239) acrD → / → yffB aminoglycoside/multidrug efflux system/conserved hypothetical protein
RA 2,594,104 G→A H138Y (CAT→TAT)  ypfI ← predicted hydrolase
RA 2,599,304 G→A M57I (ATG→ATA bcp → thiol peroxidase, thioredoxin‑dependent
RA 2,636,526 C→T L261L (CTG→CTA yfgL ← protein assembly complex, lipoprotein component
RA 2,667,558 T→C intergenic (‑6/‑130) hcaR ← / → hcaE DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit
RA 2,688,036 T→C I43V (ATT→GTT)  yfhG ← conserved hypothetical protein
RA 2,697,845 G→A G144D (GGT→GAT)  yfhH → predicted DNA‑binding transcriptional regulator
RA 2,717,229 G→A intergenic (‑45/‑162) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2
RA 2,732,036 T→A D265V (GAT→GTT)  clpB ← protein disaggregation chaperone
RA 2,759,336 C→T T45I (ACA→ATA)  yfjJ → hypothetical protein
RA 2,763,072 T→A Q246H (CAA→CAT yfjL ← hypothetical protein
RA 2,773,435 T→C L488L (TTG→CTG)  yfjW → predicted inner membrane protein
RA 2,794,102 G→A L398L (TTG→TTA gabP → gamma‑aminobutyrate transporter
RA 2,821,480 T→C T316A (ACC→GCC)  recA ← DNA strand exchange and recombination protein with protease and nuclease activity
RA 2,840,761 A→G E372E (GAA→GAG ascB → cryptic 6‑phospho‑beta‑glucosidase
RA 2,860,110 G→A V9I (GTC→ATC)  ygbJ → predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain
RA 2,866,110 T→A intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
RA 2,867,123 G→A P96L (CCG→CTG)  nlpD ← predicted outer membrane lipoprotein
RA 2,876,372 G→A intergenic (+98/+853) iap → / ← ygbF aminopeptidase in alkaline phosphatase isozyme conversion/hypothetical protein
RA 2,910,539 C→A R590L (CGC→CTC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,916,386 G→A E892K (GAA→AAA)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,985,254 G→A S251N (AGC→AAC)  yqeG → predicted transporter
RA 3,013,681 G→A V11V (GTC→GTT yqeC ← conserved hypothetical protein
RA 3,021,506 C→T T512I (ACT→ATT)  xdhD → fused predicted xanthine/hypoxanthine oxidase, molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,100,581 C→T C70Y (TGC→TAC)  yggL ← hypothetical protein
RA 3,125,146 C→T L11L (CTG→CTA glcE ← glycolate oxidase FAD binding subunit
RA 3,135,166 A→G intergenic (‑139/+153) pitB ← / ← gss phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase
RA 3,186,703 T→C F216F (TTT→TTC yqiG → predicted outer membrane usher protein
RA 3,194,164 T→C T416A (ACG→GCG)  rfaE ← fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
RA 3,205,103 A→G intergenic (‑191/‑16) ygiP ← / → ttdA predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit
RA 3,225,037 (T)6→5 coding (148/1434 nt) ygjI → predicted transporter
MC JC 3,261,134 Δ1,199 bp insH[tdcD] insH, [tdcD]
RA 3,272,678 A→T S245S (TCT→TCA garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
RA 3,358,271 A→G K1262R (AAA→AGA)  gltB → glutamate synthase, large subunit
RA 3,363,356 G→A S232N (AGC→AAC)  yhcD → predicted outer membrane protein
RA 3,400,733 A→G I70I (ATT→ATC mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,447,843 A→G V1163V (GTT→GTC rpoC ← RNA polymerase, beta prime subunit
RA 3,459,166 G→A A53V (GCT→GTT)  secE ← preprotein translocase membrane subunit
RA 3,518,471 T→C intergenic (‑305/‑120) yiiU ← / → glpF conserved hypothetical protein/glycerol facilitator
RA 3,573,836 A→G V200A (GTG→GCG)  yihN ← predicted transporter
RA 3,587,198 C→T E840K (GAA→AAA)  polA ← fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
RA 3,679,440 A→G I194V (ATT→GTT)  ilvY → DNA‑binding transcriptional dual regulator
RA 3,682,573 G→A L211L (CTG→TTG)  ilvD ← dihydroxyacid dehydratase
MC JC 3,742,938 Δ1,336 bp insDinsC insD, insC
MC JC 3,746,913 Δ1,199 bp [tnaB][tnaB] [tnaB], insH, [tnaB]
MC JC 3,750,593 Δ1,199 bp insH insH
RA 3,792,109 Δ1 bp coding (317/1320 nt) uhpC → membrane protein regulates uhpT expression
RA 3,815,914 T→C N702D (AAT→GAT)  spoT ← bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,824,280 T→C pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,537 +G pseudogene (670/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,989,743 (G)7→6 intergenic (‑617/+12) yhiS ← / ← arsC hypothetical protein/arsenate reductase
RA 4,066,786 C→T intergenic (+144/‑129) asd → / → glgB aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme
RA 4,071,423 +A coding (335/1296 nt) glgC → glucose‑1‑phosphate adenylyltransferase
RA 4,103,301 A→G R102R (CGT→CGC greB ← transcription elongation factor
RA 4,131,742 (G)5→6 coding (1138/1227 nt) yhfW → predicted mutase
RA 4,154,666 A→G I23V (ATT→GTT)  yhfA → conserved hypothetical protein
RA 4,155,147 +A coding (782/870 nt) prkB ← predicted phosphoribulokinase
RA 4,268,686 C→T L261L (CTG→TTG)  dnaB → replicative DNA helicase
RA 4,271,811 A→G Y369C (TAT→TGT)  tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,292,897 C→T L21L (CTG→TTG)  nrfB → nitrite reductase, formate‑dependent, penta‑heme cytochrome c
RA 4,295,565 T→C F154F (TTT→TTC nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
MC JC 4,310,365 Δ1,199 bp insH[alsK] insH, [alsK]
RA 4,371,276 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,421,418 C→T R12Q (CGG→CAG)  yjfO ← conserved hypothetical protein
RA 4,428,265 +G coding (738/855 nt) ulaE → L‑xylulose 5‑phosphate 3‑epimerase
RA 4,486,264 C→T P752P (CCG→CCA valS ← valyl‑tRNA synthetase
RA 4,491,067 G→A V56V (GTG→GTA yjgP → conserved inner membrane protein
RA 4,501,500 A→G Q23Q (CAA→CAG intB → predicted integrase
RA 4,501,608 A→G K59K (AAA→AAG intB → predicted integrase
RA 4,549,749 C→T intergenic (+38/‑29) fimC → / → fimD chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis
RA 4,553,062 T→C G32G (GGT→GGC fimG → minor component of type 1 fimbriae
RA 4,563,589 T→A K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 282911 282912 2 3 [0] [0] 4 yagF predicted dehydratase
* * ÷ W3110S.gb 283057 283151 95 2 [1] [1] 2 yagF predicted dehydratase
* * ÷ W3110S.gb 285753 285780 28 9 [0] [1] 2 yagG predicted sugar transporter
* * ÷ W3110S.gb 286788 286852 65 2 [1] [0] 5 yagH predicted xylosidase/arabinosidase
* * ÷ W3110S.gb 287731 287761 31 5 [1] [0] 3 yagI predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 340898 340901 4 4 [0] [0] 12 yahJ predicted deaminase with metallo‑dependent hydrolase domain
* * ÷ W3110S.gb 422758 422775 18 6 [0] [0] 3 malZ maltodextrin glucosidase
* * ÷ W3110S.gb 509172 509192 21 5 [1] [0] 2 copA copper transporter
* * ÷ W3110S.gb 573875–575007 575999 993–2125 6 [0] [0] 89 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 595285 595288 4 5 [0] [0] 3 cusC copper/silver efflux system, outer membrane component
* * ÷ W3110S.gb 598767 598800 34 2 [1] [0] 2 cusA copper/silver efflux system, membrane component
* * ÷ W3110S.gb 721523 721551 29 2 [0] [0] 6 kdpE DNA‑binding response regulator in two‑component regulatory system with KdpD
* * ÷ W3110S.gb 724431 724440 10 4 [0] [0] 3 kdpD fused sensory histidine kinase in two‑component regulatory system with KdpE
* * ÷ W3110S.gb 743323 743341 19 2 [0] [1] 7 ybgI conserved metal‑binding protein
* * ÷ W3110S.gb 837465 837473 9 4 [0] [1] 8 ybiC predicted dehydrogenase
* * ÷ W3110S.gb 845731 845735 5 3 [0] [1] 4 ybiO predicted mechanosensitive channel
* * ÷ W3110S.gb 860911 860914 4 5 [0] [1] 3 ybiW predicted pyruvate formate lyase
* * ÷ W3110S.gb 911777 911808 32 12 [1] [0] 5 hcr HCP oxidoreductase, NADH‑dependent
* * ÷ W3110S.gb 939927 939928 2 3 [0] [0] 4 serS seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine
* * ÷ W3110S.gb 976968 976970 3 5 [0] [0] 10 mukB fused chromosome partitioning proteins
* * ÷ W3110S.gb 982202 982216 15 3 [0] [0] 14 ycbB predicted carboxypeptidase
* * ÷ W3110S.gb 1023056 1023060 5 3 [0] [0] 2 yccS predicted inner membrane protein
* * ÷ W3110S.gb 1030061 1030062 2 3 [0] [0] 6 yccW predicted methyltransferase
* * ÷ W3110S.gb 1041910 1041911 2 2 [0] [0] 4 appA phosphoanhydride phosphorylase
* * ÷ W3110S.gb 1081327 1081363 37 4 [0] [0] 10 putP/ycdN proline:sodium symporter/ECK1007:JW5141:b1016; hypothetical protein, N‑ter fragment
* * ÷ W3110S.gb 1171859 1171867 9 5 [0] [0] 6 ycfS conserved hypothetical protein
* * ÷ W3110S.gb 1251331 1251380 50 2 [1] [0] 8 [dhaL]–[dhaK] [dhaL],[dhaK]
* * ÷ W3110S.gb 1328311 1328351 41 2 [1] [0] 2 yciQ predicted inner membrane protein
* * ÷ W3110S.gb 1338233 1338234 2 6 [0] [0] 4 acnA aconitate hydratase 1
* * ÷ W3110S.gb 1339007 1339007 1 9 [0] [0] 3 acnA aconitate hydratase 1
* * ÷ W3110S.gb 1352915 1352929–1352918 4–15 4 [1] [1] 5 fabI/ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein
* * ÷ W3110S.gb 1371194 1371235 42 2 [0] [1] 3 pspD peripheral inner membrane phage‑shock protein
* * ÷ W3110S.gb 1382364 1382371 8 3 [0] [0] 2 ycjU predicted beta‑phosphoglucomutase
* * ÷ W3110S.gb 1421322 1421323 2 10 [0] [0] 6 ydaG/racR hypothetical protein/predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 1431518 1431525 8 7 [0] [0] 4 stfR predicted tail fiber protein
* * ÷ W3110S.gb 1785967 1785978 12 7 [1] [1] 5 ydiD short chain acyl‑CoA synthetase, anaerobic
* * ÷ W3110S.gb 1797529 1797540 12 5 [0] [1] 3 pheT phenylalanine tRNA synthetase, beta subunit
* * ÷ W3110S.gb 1828092 1828140 49 5 [1] [0] 8 astE succinylglutamate desuccinylase
* * ÷ W3110S.gb 1904252 1904264 13 2 [0] [0] 5 manX fused mannose‑specific PTS enzyme IIAB components
* * ÷ W3110S.gb 1926080 1926091 12 6 [1] [0] 3 yebZ predicted inner membrane protein
* * ÷ W3110S.gb 2015095 2015109 15 5 [0] [0] 11 fliE flagellar basal‑body component
* * ÷ W3110S.gb 2041774 2041808 35 3 [0] [0] 3 yedY predicted reductase
* * ÷ W3110S.gb 2048443 2048443 1 21 [0] [0] 2 yeeJ adhesin
* * ÷ W3110S.gb 2061111 2061126 16 5 [1] [1] 2 yeeO predicted multidrug efflux system
* * ÷ W3110S.gb 2070543 2070548 6 2 [1] [0] 2 insH/yoeA IS5 transposase and trans‑activator/CP4‑44 prophage region; ECK1989:JW5326:b1995; predicted disrupted hemin or colicin receptor
* * ÷ W3110S.gb 2129599 2129602 4 3 [0] [0] 8 gmd GDP‑D‑mannose dehydratase, NAD(P)‑binding
* * ÷ W3110S.gb 2181529 2181530 2 12 [1] [0] 25 fbaB fructose‑bisphosphate aldolase class I
* * ÷ W3110S.gb 2296359 2296415 57 6 [0] [1] 4 ccmF heme lyase, CcmF subunit
* * ÷ W3110S.gb 2303453 2303491 39 4 [0] [1] 4 napG ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)
* * ÷ W3110S.gb 2569114 2569126 13 2 [1] [0] 9 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ W3110S.gb 2571609 2571610 2 6 [0] [0] 2 eutI predicted phosphotransacetylase subunit
* * ÷ W3110S.gb 2662101 2662120 20 4 [0] [0] 3 suhB inositol monophosphatase
* * ÷ W3110S.gb 2714522 2714535 14 7 [0] [0] 2 yfiK neutral amino‑acid efflux system
* * ÷ W3110S.gb 2827377 2827403 27 3 [0] [0] 3 gutM DNA‑binding transcriptional activator
* * ÷ W3110S.gb 3566207 3566208 2 2 [1] [0] 3 yihS/yihR predicted glucosamine isomerase/predicted aldose‑1‑epimerase
* * ÷ W3110S.gb 3799024 3799047 24 3 [0] [0] 4 yicM predicted transporter
* * ÷ W3110S.gb 3859266 3859315 50 5 [1] [1] 11 yibK/lldD predicted rRNA methylase/L‑lactate dehydrogenase, FMN‑linked
* * ÷ W3110S.gb 3912257 3912258 2 2 [0] [0] 7 xylB xylulokinase
* * ÷ W3110S.gb 4077337 4077340 4 3 [0] [1] 3 glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
* * ÷ W3110S.gb 4213641–4215889 4216699–4215891 3–3059 3 [0] [1] 3 [rrlE]–[rrfE] [rrlE],[rrfE]
* * ÷ W3110S.gb 4401147 4401160 14 3 [1] [0] 7 amiB N‑acetylmuramoyl‑l‑alanine amidase II
* * ÷ W3110S.gb 4522152 4522158 7 2 [0] [1] 3 fecR transmembrane signal transducer for ferric citrate transport
* * ÷ W3110S.gb 4619299–4619315 4619315 1–17 3 [0] [1] 13 yjjV/yjjW predicted DNase/predicted pyruvate formate lyase activating enzyme

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 274373NA (NA)89 (1.660) 5/126 0.9 100% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 0 (0.000)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb 314453 =56 (1.040)63 (1.210)
+TC
11/122 0.3 69.9% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb 3932930 = 0 (0.000)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 315707NA (NA)90 (1.680) 15/126 0.2 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 566000 =NA (NA)30 (0.560) 7/126 0.7 66.7% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 15 (0.280)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 2018571 =0 (0.000)45 (0.880) 10/120 0.4 100% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 20185834 (0.070)51 (0.980) 8/122 0.5 93.0% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb 2172308 =NA (NA)44 (0.830) 10/124 0.4 88.2% pseudogene (1563/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb = 3920281 6 (0.110)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173564NA (NA)14 (0.270) 7/124 0.6 87.7% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 2 (0.040)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173565NA (NA)55 (1.020) 15/126 0.2 98.2% pseudogene (306/2041 nt)
pseudogene (306/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb = 3932933 1 (0.020)coding (222/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2412225NA (NA)112 (2.090) 21/126 0.0 96.5% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 4 (0.070)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb 3748799 =15 (0.280)60 (1.120) 17/126 0.1 80.0% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb 4547364 =11 (0.200)16 (0.350) 5/108 0.8 58.8% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb 4547678 = 13 (0.280)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? W3110S.gb = 454737211 (0.200)10 (0.220) 4/108 1.0 47.2% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb = 4547668 13 (0.280)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin