Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,667,558 |
T→C |
intergenic (‑6/‑130) |
hcaR ← / → hcaE |
DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,667,558 | 0 | T | C | 100.0%
| 95.8
/ NA
| 27 | intergenic (‑6/‑130) | hcaR/hcaE | DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/13); total (14/13) |
CGCACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAG > W3110S.gb/2667490‑2667615
|
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:4034421/71‑1 (MQ=255)
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:3380901/71‑1 (MQ=255)
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:2836466/71‑1 (MQ=255)
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:2593171/71‑1 (MQ=255)
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:1553400/71‑1 (MQ=255)
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:1663560/71‑1 (MQ=255)
cgcACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTccc < 1:2220781/71‑1 (MQ=255)
gTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGt > 1:1390701/1‑70 (MQ=255)
gTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGt > 1:2306051/1‑70 (MQ=255)
gTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGt > 1:2258962/1‑70 (MQ=255)
cTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGtc < 1:1187700/71‑1 (MQ=255)
cTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGtc < 1:83205/71‑1 (MQ=255)
cTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGtc < 1:2397803/71‑1 (MQ=255)
gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt > 1:745835/1‑55 (MQ=255)
gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt > 1:242455/1‑55 (MQ=255)
gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt > 1:2602866/1‑55 (MQ=255)
gaAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt > 1:3031930/1‑55 (MQ=255)
tAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAAc < 1:3776916/71‑1 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGt > 1:2978320/1‑46 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTCACTAttt < 1:3256982/71‑1 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:1667356/71‑1 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:1292519/71‑1 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:1306362/71‑1 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt > 1:2878550/1‑71 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt > 1:1373209/1‑71 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAttt < 1:2149447/71‑1 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAtt > 1:1587179/1‑70 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAtt > 1:4079103/1‑70 (MQ=255)
tAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTAtt > 1:900428/1‑70 (MQ=255)
tGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca < 1:1402080/71‑1 (MQ=255)
tGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca < 1:1184625/71‑1 (MQ=255)
cGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACatat < 1:664667/71‑1 (MQ=255)
cGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACatat < 1:826350/71‑1 (MQ=255)
gTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAg > 1:3380209/1‑70 (MQ=255)
|
CGCACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAG > W3110S.gb/2667490‑2667615
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
