Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,459,166 |
G→A |
A53V (GCT→GTT) |
secE ← |
preprotein translocase membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,459,166 | 0 | G | A | 100.0%
| 178.5
/ NA
| 57 | A53V (GCT→GTT) | secE | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/31); total (26/31) |
GCGCTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAGCAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAATGTCGCGATAAAGATAGTTGCCGACA > W3110S.gb/3459098‑3459234
|
gcgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTgcaaca < 1:221410/71‑1 (MQ=255)
gcgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTgcaaca < 1:3473882/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:1036695/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:152252/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:4013989/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:3887108/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:3840430/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:3710881/71‑1 (MQ=255)
cgcTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:3807939/71‑1 (MQ=255)
aCGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGa < 1:2315759/70‑1 (MQ=255)
aCGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGa < 1:1963386/70‑1 (MQ=255)
aCGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGa < 1:3550054/70‑1 (MQ=255)
aCGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGa < 1:1656310/70‑1 (MQ=255)
aCGGGAAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGa < 1:2958535/70‑1 (MQ=255)
ggCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATc > 1:2286082/1‑64 (MQ=255)
ggCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATta > 1:165086/1‑71 (MQ=255)
ggCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATta > 1:3342076/1‑71 (MQ=255)
ggCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATta > 1:820088/1‑71 (MQ=255)
ggCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGAGACACCCCCCGCTGCAACAATCAGAATta > 1:181729/1‑71 (MQ=255)
aaGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGa < 1:4020530/62‑1 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATc > 1:1290176/1‑57 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:3352596/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:1692413/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:1655173/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:1882710/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:3659755/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:2015647/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:3460587/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:3022416/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:2763502/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:2558912/1‑71 (MQ=255)
gCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGc > 1:67200/1‑71 (MQ=255)
aCGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGcaacaa < 1:2627427/52‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCTCGACACCACCCGCTGCAACAATCAg < 1:3035351/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCGACAATCAg < 1:580044/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:985921/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:2932052/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:3807362/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:2412992/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:1349016/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:1551799/55‑1 (MQ=255)
cGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAg < 1:544012/55‑1 (MQ=255)
gTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGGTGCAACAATCAGaa > 1:3432752/1‑55 (MQ=255)
gTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGaa > 1:3957098/1‑55 (MQ=255)
gTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGaa > 1:2726669/1‑55 (MQ=255)
gTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGaa > 1:3936348/1‑55 (MQ=255)
tAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGcgca < 1:1885803/71‑1 (MQ=255)
cTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGgc < 1:3692777/71‑1 (MQ=255)
cTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGgc < 1:4041538/71‑1 (MQ=255)
cTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGgc < 1:3819676/71‑1 (MQ=255)
cTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGgc < 1:2749390/71‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:2437342/70‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:3531890/70‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:3971159/70‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:978972/70‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:20304/70‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:264674/70‑1 (MQ=255)
tCGTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:2268683/70‑1 (MQ=255)
gTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAAt > 1:1820922/1‑71 (MQ=255)
gTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAAt > 1:863559/1‑71 (MQ=255)
gTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAAt > 1:710766/1‑71 (MQ=255)
gTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAAt > 1:3173895/1‑71 (MQ=255)
gTTAACAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAAt > 1:1437847/1‑71 (MQ=255)
cAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAATGt < 1:602402/68‑1 (MQ=255)
cAGCGCGACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAATGt < 1:498209/68‑1 (MQ=255)
cgACACCACCCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAATGt < 1:4139630/63‑1 (MQ=255)
ccaccCGCTGCAACAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCAt < 1:3385061/53‑1 (MQ=255)
cagcaATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAATGTCGCGATAAAGATAGTTGCCGACa < 1:3680370/71‑1 (MQ=255)
|
GCGCTTCACGGGCAAAAGCAACGGTAGCTTTACCTTTTGTCGTTAACAGCGCGACACCACCCGCTGCAGCAATCAGAATTACTACGGCCAGCGCACGCAGCGGCAGCATAATGTCGCGATAAAGATAGTTGCCGACA > W3110S.gb/3459098‑3459234
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
