Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
899,672 |
T→C |
21.9% |
L15P (CTG→CCG) |
pspE → |
thiosulfate:cyanide sulfurtransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 899,672 | 0 | T | C | 21.9%
| 195.3
/ 55.3
| 96 | L15P (CTG→CCG) | pspE | thiosulfate:cyanide sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base T (41/34); new base C (10/11); total (51/45) |
| Fisher's exact test for biased strand distribution p-value = 6.26e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
AATTTTCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTC > minE/899602‑899740
|
aaTTTTCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACt > 1:2853038/1‑71 (MQ=255)
aaTTTTCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACt > 1:1674951/1‑71 (MQ=255)
aTTTTCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTg > 1:4110586/1‑71 (MQ=255)
ttttCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGc > 1:2253094/1‑71 (MQ=255)
ttCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGccc > 1:1187676/1‑71 (MQ=255)
tCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCg > 1:1339472/1‑71 (MQ=255)
tCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCg > 1:2802077/1‑71 (MQ=255)
tCATACATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGccc > 1:1258939/1‑70 (MQ=255)
aTCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGtt < 1:1831720/71‑1 (MQ=255)
cATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGtttt < 1:282394/70‑1 (MQ=255)
gAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTTCCCGTTTTcgcc < 1:999460/70‑1 (MQ=255)
gAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTTCCCGTTTTcgcc < 1:3750951/70‑1 (MQ=255)
gAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTcgcc < 1:3867457/70‑1 (MQ=255)
gAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTcgcc < 1:3643922/70‑1 (MQ=255)
ggACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTg < 1:1385180/71‑1 (MQ=255)
ggACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTg < 1:30263/71‑1 (MQ=255)
gACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGa < 1:2393291/71‑1 (MQ=255)
cGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAac < 1:656659/71‑1 (MQ=255)
tttAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCg < 1:2500917/70‑1 (MQ=255)
ttAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGaa < 1:3667855/59‑1 (MQ=255)
ttAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGAt > 1:3879977/1‑71 (MQ=255)
tAAAAAGGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:542243/1‑71 (MQ=255)
tAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:3945242/1‑71 (MQ=255)
tAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:3184012/1‑71 (MQ=255)
tAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:1705725/1‑71 (MQ=255)
aaaaaaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTcgc > 1:3875342/1‑50 (MQ=255)
aaaaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGc < 1:4214504/38‑1 (MQ=255)
aaaaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGc < 1:2452887/38‑1 (MQ=255)
aaaaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTc > 1:3929391/1‑71 (MQ=255)
aaaaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTc > 1:3121831/1‑71 (MQ=255)
aaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTc > 1:31702/1‑44 (MQ=255)
aaGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCgt < 1:3589835/71‑1 (MQ=255)
cTTACTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGtt > 1:2112625/1‑70 (MQ=255)
aCTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAaca > 1:1905512/1‑48 (MQ=255)
cTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGaa > 1:1446593/1‑45 (MQ=255)
cTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGaa > 1:1242015/1‑45 (MQ=255)
cTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGaa > 1:1488700/1‑45 (MQ=255)
cTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCag < 1:3740876/70‑1 (MQ=255)
cTTGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATCTTCGTGTTCCag < 1:58908/70‑1 (MQ=255)
cTTGCTCTGGCACTGGTGTTTTCACCGCCCGTCTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCag < 1:865147/70‑1 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATc < 1:1694324/53‑1 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATc < 1:2951732/53‑1 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:2692740/1‑71 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:2547711/1‑71 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:3402059/1‑71 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:2666025/1‑71 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:964083/1‑71 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCaga > 1:869599/1‑70 (MQ=255)
ttGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCag > 1:4222468/1‑69 (MQ=255)
tGCTCTGGCACTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:2592626/1‑70 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag < 1:1770664/69‑1 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:1460377/1‑69 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:4155046/1‑69 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag < 1:2695875/69‑1 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCagag > 1:589137/1‑69 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGc < 1:2110549/70‑1 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGc < 1:585265/70‑1 (MQ=255)
gCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGc < 1:3085268/70‑1 (MQ=255)
tctGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTcc > 1:1588973/1‑63 (MQ=255)
tctGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTcc > 1:2221842/1‑63 (MQ=255)
tctGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTcc > 1:3868698/1‑63 (MQ=255)
tctGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTcc > 1:1780545/1‑63 (MQ=255)
tctGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTcc > 1:1354717/1‑63 (MQ=255)
gCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTAt < 1:3951591/69‑1 (MQ=255)
gCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTAt > 1:467954/1‑69 (MQ=255)
gCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTAt > 1:2904664/1‑69 (MQ=255)
gCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTAt < 1:577329/69‑1 (MQ=255)
gCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTAt < 1:2805925/69‑1 (MQ=255)
aCTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCg < 1:3267146/51‑1 (MQ=255)
cTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCa < 1:1891399/71‑1 (MQ=255)
cTGGTGTTTTCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCa < 1:3899413/71‑1 (MQ=255)
tGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:1829885/1‑45 (MQ=255)
tGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:3142369/1‑45 (MQ=255)
tGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:1959078/1‑45 (MQ=255)
tGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATg > 1:2682987/1‑45 (MQ=255)
tGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCgtg > 1:21085/1‑51 (MQ=255)
gtgtTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAag < 1:3288631/70‑1 (MQ=255)
ttttCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCgt < 1:1706569/45‑1 (MQ=255)
ttttCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCgt < 1:3290527/45‑1 (MQ=255)
ttttCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCgt < 1:407799/45‑1 (MQ=255)
ttttCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCa < 1:3032676/71‑1 (MQ=255)
ttCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:2937631/1‑70 (MQ=255)
ttCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACg > 1:146253/1‑71 (MQ=255)
tCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:206264/1‑69 (MQ=255)
tCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:593895/1‑69 (MQ=255)
tCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:3675365/1‑69 (MQ=255)
tCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:3200008/1‑69 (MQ=255)
tCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:4022009/1‑69 (MQ=255)
tCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCAc > 1:3354023/1‑69 (MQ=255)
tCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACg < 1:3169548/70‑1 (MQ=255)
tCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACg < 1:395561/70‑1 (MQ=255)
tCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACg < 1:3279131/70‑1 (MQ=255)
tCACCGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACg < 1:3353920/70‑1 (MQ=255)
aCTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTc < 1:1450144/71‑1 (MQ=255)
aCTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTc < 1:710735/71‑1 (MQ=255)
aCTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTc < 1:3495540/71‑1 (MQ=255)
aCTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTc < 1:3706235/71‑1 (MQ=255)
aCTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTc < 1:116511/71‑1 (MQ=255)
|
AATTTTCATCCATAGAAGGACGCTTACATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTC > minE/899602‑899740
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
