breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 60,568 G→A 43.1% R125R (CGC→CGT hepA ← RNA polymerase‑associated helicase protein
RA 138,172 A→G 52.8% N665N (AAT→AAC htrE ← predicted outer membrane usher protein
RA 158,183 A→G 75.8% N41S (AAC→AGC)  dgt → deoxyguanosine triphosphate triphosphohydrolase
RA 163,666 C→T 42.4% E77K (GAA→AAA)  yaeI ← predicted phosphatase
RA 199,135 T→A 35.4% S207C (AGC→TGC)  metQ ← DL‑methionine transporter subunit
RA 204,511 C→T 100% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 216,053 T→C 28.1% intergenic (+222/‑106) aspV → / → yafT tRNA‑Asp/predicted aminopeptidase
RA 247,251 A→G 8.3% K298K (AAA→AAG phoR → sensory histidine kinase in two‑component regulatory system with PhoB
RA 249,414 A→C 12.6% intergenic (+35/‑41) brnQ → / → proY predicted branched chain amino acid transporter/predicted cryptic proline transporter
RA 286,800 T→C 46.7% P302P (CCT→CCC clpX → ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
RA 297,667 C→T 32.2% R110C (CGT→TGT)  mdlA → fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily
RA 298,537 C→T 45.5% L400L (CTG→TTG)  mdlA → fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily
RA 300,220 G→A 51.6% L372L (CTG→CTA mdlB → fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily
RA 335,162 G→A 78.3% G130G (GGC→GGT ybaK ← conserved hypothetical protein
RA 374,214 G→A 57.4% G355D (GGT→GAT)  fepE → regulator of length of O‑antigen component of lipopolysaccharide chains
RA 402,650 C→T 45.0% G38D (GGC→GAC)  rlpA ← minor lipoprotein
RA 430,231 G→A 45.8% D280N (GAC→AAC)  glnS → glutamyl‑tRNA synthetase
RA 447,412 G→A 100% R102C (CGC→TGC)  kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE
RA 498,441 C→T 63.6% G24S (GGT→AGT)  galE ← UDP‑galactose‑4‑epimerase
RA 507,425 C→A 32.2% A71D (GCT→GAT)  ybhH → conserved hypothetical protein
RA 555,000 G→A 10.7% D148N (GAC→AAC)  fsaA → fructose‑6‑phosphate aldolase 1
RA 558,918 T→C 26.4% P55P (CCT→CCC cmr → multidrug efflux system protein
RA 581,876 G→A 24.7% P638P (CCG→CCA clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 597,552 C→T 54.2% intergenic (+133/‑106) serS → / → dmsA seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 598,845 C→T 19.3% I396I (ATC→ATT dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A
RA 623,375 C→T 67.6% T19I (ACC→ATC)  msbA → fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily
RA 656,044 T→C 18.4% F44L (TTC→CTC)  ycbS → predicted outer membrane usher protein
RA 694,025 T→A 7.7% L193* (TTA→TAA)  hyaF → protein involved in nickel incorporation into hydrogenase‑1 proteins
RA 696,779 C→T 52.1% P262L (CCC→CTC)  appB → cytochrome bd‑II oxidase, subunit II
RA 813,910 A→G 44.6% C60C (TGT→TGC prsA ← phosphoribosylpyrophosphate synthase
RA 868,233 C→G 5.9% intergenic (‑167/‑53) yciK ← / → sohB predicted oxoacyl‑(acyl carrier protein) reductase, EmrKY‑TolC system/predicted inner membrane peptidase
RA 879,563 T→C 47.2% Y18H (TAT→CAT)  yciM → conserved hypothetical protein
RA 880,753 G→A 66.0% intergenic (+72/‑122) yciM → / → pyrF conserved hypothetical protein/orotidine‑5'‑phosphate decarboxylase
RA 899,197 C→T 55.3% P78L (CCT→CTT)  pspC → transcriptional activator
RA 899,672 T→C 21.9% L15P (CTG→CCG)  pspE → thiosulfate:cyanide sulfurtransferase
RA 904,512 G→T 37.2% L161L (CTG→CTT ycjP → predicted sugar transporter subunit
RA 904,516 A→T 28.8% S163C (AGC→TGC)  ycjP → predicted sugar transporter subunit
RA 909,573 C→T 46.5% L584L (CTG→TTG)  ycjT → predicted hydrolase
RA 1,010,827 C→T 6.0% Y85Y (TAC→TAT rstB → sensory histidine kinase in two‑component regulatory system with RstA
RA 1,010,832 T→A 7.1% L87H (CTT→CAT)  rstB → sensory histidine kinase in two‑component regulatory system with RstA
RA 1,011,234 A→G 41.1% H221R (CAC→CGC)  rstB → sensory histidine kinase in two‑component regulatory system with RstA
RA 1,026,348 T→C 50.9% E175G (GAG→GGG)  malI ← DNA‑binding transcriptional repressor
RA 1,027,446 A→G 46.2% N134S (AAC→AGC)  malX → fused maltose and glucose‑specific PTS enzyme IIBC components
RA 1,036,505 G→A 100% A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,063,788 T→A 11.6% intergenic (+138/+24) sodB → / ← ydhP superoxide dismutase, Fe/predicted transporter
RA 1,063,791 T→A 12.5% intergenic (+141/+21) sodB → / ← ydhP superoxide dismutase, Fe/predicted transporter
RA 1,069,991 G→A 41.3% Q296Q (CAG→CAA cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,073,671 C→T 52.2% G50S (GGC→AGC)  ydhQ ← conserved hypothetical protein
RA 1,094,567 T→C 58.7% I604V (ATT→GTT)  ydiJ ← predicted FAD‑linked oxidoreductase
RA 1,104,294 A→T 10.2% G339G (GGA→GGT ydiF → fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit
RA 1,111,527 G→A 50.9% C287Y (TGT→TAT)  ydiD → short chain acyl‑CoA synthetase, anaerobic
RA 1,132,803 G→A 40.0% intergenic (+119/‑38) arpB → / → yniD ECK1718:JW5278+JW1710:b4494; hypothetical protein/hypothetical protein
RA 1,142,011 T→C 43.0% F152L (TTC→CTC)  katE → hydroperoxidase HPII(III)
RA 1,142,959 A→G 57.1% N468D (AAC→GAC)  katE → hydroperoxidase HPII(III)
RA 1,179,007 A→T 13.2% N153Y (AAC→TAC)  ansA → cytoplasmic L‑asparaginase I
RA 1,215,064 G→A 60.4% L291L (CTG→CTA yebQ → predicted transporter
RA 1,224,676 T→C 46.0% F180L (TTT→CTT)  yebU → predicted methyltransferase
RA 1,240,151 A→G 60.0% L27P (CTG→CCG)  zwf ← glucose‑6‑phosphate dehydrogenase
RA 1,249,224 C→T 45.2% D17N (GAT→AAT)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome
RA 1,257,376 T→C 54.1% F7L (TTT→CTT)  yecP → predicted methyltransferase
RA 1,291,126 G→A 66.3% intergenic (+241/+212) yeeN → / ← asnW conserved hypothetical protein/tRNA‑Asn
RA 1,303,538 T→C 60.9% F84L (TTT→CTT)  sbcB → exonuclease I
RA 1,328,372 G→A 51.5% F131F (TTC→TTT wzxC ← colanic acid exporter
RA 1,408,722 G→A 35.0% A331T (GCG→ACG)  yejF → fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily
RA 1,418,334 G→A 66.2% V95I (GTC→ATC)  narP → DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX
RA 1,475,665 A→T 48.6% T244S (ACC→TCC)  nrdB → ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like
RA 1,485,005 C→T 19.1% D279N (GAT→AAT)  menF ← isochorismate synthase 2
RA 1,496,561 C→T 41.8% M327I (ATG→ATA nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 1,511,807 G→A 53.7% E359K (GAG→AAG)  pta → phosphate acetyltransferase
RA 1,523,433 G→T 37.5% A78S (GCC→TCC)  flk → predicted flagella assembly protein
RA 1,533,542 G→A 41.5% P51S (CCT→TCT)  prmB ← N5‑glutamine methyltransferase
RA 1,569,653 G→C 11.6% intergenic (‑328/‑8) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter
RA 1,569,654 A→T 11.8% intergenic (‑329/‑7) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter
RA 1,569,655 G→C 11.8% intergenic (‑330/‑6) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter
RA 1,598,488 C→T 50.8% A60A (GCG→GCA yfeT ← predicted DNA‑binding transcriptional regulator
RA 1,697,632 A→G 44.7% Y195C (TAT→TGT)  yfhR → predicted peptidase
RA 1,718,872 G→C 9.8% D118H (GAC→CAC)  hmp → fused nitric oxide dioxygenase and dihydropteridine reductase 2
RA 1,747,876 G→A 26.2% S44L (TCA→TTA)  yfiE ← predicted DNA‑binding transcriptional regulator
RA 1,763,556 T→C 54.6% noncoding (288/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,636 A→G 27.3% noncoding (208/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,789,346 Δ1 bp 43.7% coding (483/1269 nt) ygaF → hypothetical protein
RA 1,798,695 A→G 46.7% Q239R (CAA→CGA)  proV → glycine betaine transporter subunit
RA 1,803,598 T→C 27.0% N30N (AAT→AAC ygaH → predicted inner membrane protein
RA 1,807,377 T→C 26.1% intergenic (+58/+6) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteinase
RA 1,822,088 C→T 7.4% S465S (TCC→TCT norV → flavorubredoxin oxidoreductase
RA 1,827,932 C→T 57.1% A392V (GCG→GTG)  mutS → methyl‑directed mismatch repair protein
RA 1,834,094 A→G 33.3% D65G (GAT→GGT)  ygbM → conserved hypothetical protein
RA 1,845,199 G→T 58.8% P266T (CCG→ACG)  cysD ← sulfate adenylyltransferase, subunit 2
RA 1,874,329 A→G 37.5% I191T (ATT→ACT)  ygcF ← conserved hypothetical protein
RA 1,883,198 A→G 50.4% F429L (TTC→CTC)  rumA ← 23S rRNA (uracil‑5)‑methyltransferase
RA 1,890,961 G→A 69.4% P208S (CCG→TCG)  gudP ← predicted D‑glucarate transporter
RA 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 1,914,848 T→C 56.8% E208E (GAA→GAG ptsP ← fused PEP‑protein phosphotransferase (enzyme I) of PTS system
RA 1,983,953 G→C 12.7% A93A (GCG→GCC yggV → dITP/XTP pyrophosphatase
RA 1,993,572 G→A 42.6% V289M (GTG→ATG)  nupG → nucleoside transporter
RA 2,004,302 G→A 51.3% S483F (TCT→TTT)  hybC ← hydrogenase 2, large subunit
RA 2,065,298 T→C 7.8% D513D (GAT→GAC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,301 T→C 7.8% D514D (GAT→GAC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,304 A→C 7.6% E515D (GAA→GAC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,065,319 G→C 8.2% G520G (GGG→GGC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 2,092,381 C→T 36.2% A257T (GCC→ACC)  yhaO ← predicted transporter
RA 2,101,664 A→C 7.4% T432T (ACA→ACC garD → (D)‑galactarate dehydrogenase
RA 2,124,793 C→T 6.4% Q70Q (CAG→CAA yhbP ← conserved hypothetical protein
RA 2,144,345 G→A 45.0% D206N (GAT→AAT)  argG → argininosuccinate synthetase
RA 2,168,106 T→A 5.0% Y60N (TAT→AAT)  yrbK → conserved hypothetical protein
RA 2,168,110 G→A 5.3% R61Q (CGA→CAA)  yrbK → conserved hypothetical protein
RA 2,168,113 T→C 5.2% L62S (TTG→TCG)  yrbK → conserved hypothetical protein
RA 2,168,117 T→A 5.2% I63I (ATT→ATA yrbK → conserved hypothetical protein
RA 2,211,683 A→G 37.9% I465I (ATT→ATC rng ← ribonuclease G
RA 2,236,903 G→A 45.9% A150T (GCT→ACT)  purD → phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
RA 2,262,618 C→T 44.3% intergenic (‑227/+186) rplJ ← / ← rplA 50S ribosomal subunit protein L10/50S ribosomal subunit protein L1
RA 2,283,783 G→T 41.5% L39M (CTG→ATG)  oxyR ← DNA‑binding transcriptional dual regulator
RA 2,321,698 G→A 52.7% T93I (ACC→ATC)  cdh ← CDP‑diacylglycerol phosphotidylhydrolase
RA 2,348,624 T→G 9.8% intergenic (+144/+237) yihA → / ← polA GTP‑binding protein/fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
RA 2,348,627 C→A 21.9% intergenic (+147/+234) yihA → / ← polA GTP‑binding protein/fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
RA 2,359,867 G→A 21.7% noncoding (1228/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,359,876 C→T 20.0% noncoding (1219/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,377,802 A→T 31.7% Y196N (TAC→AAC)  ubiB ← 2‑octaprenylphenol hydroxylase
RA 2,441,897 C→T 78.2% L462L (TTG→TTA ilvA ← threonine deaminase
RA 2,467,238 G→T 31.8% L234L (CTG→CTT atpA → F1 sector of membrane‑bound ATP synthase, alpha subunit
RA 2,489,241 T→C 10.7% R80R (CGT→CGC dnaN → DNA polymerase III, beta subunit
RA 2,513,416 T→C 50.0% F203S (TTT→TCT)  gltS → glutamate transporter
RA 2,523,040 T→C 39.7% K225K (AAA→AAG dinD ← DNA‑damage‑inducible protein
RA 2,523,551 T→A 5.6% H55L (CAC→CTC)  dinD ← DNA‑damage‑inducible protein
RA 2,523,557 C→G 5.1% S53T (AGT→ACT)  dinD ← DNA‑damage‑inducible protein
RA 2,523,766 T→A 8.0% intergenic (‑52/+169) dinD ← / ← yicC DNA‑damage‑inducible protein/conserved hypothetical protein
RA 2,523,769 T→A 9.4% intergenic (‑55/+166) dinD ← / ← yicC DNA‑damage‑inducible protein/conserved hypothetical protein
RA 2,528,498 C→T 24.6% D83N (GAT→AAT)  dfp ← fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase, FMN‑binding
RA 2,530,398 G→A 31.7% A26T (GCA→ACA)  mutM → formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
RA 2,531,609 A→G 64.2% I14I (ATT→ATC coaD ← pantetheine‑phosphate adenylyltransferase
RA 2,540,373 G→T 35.8% D300Y (GAT→TAT)  rfaJ → UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase
RA 2,599,135 A→T 29.5% I33I (ATT→ATA gor ← glutathione oxidoreductase
RA 2,651,936 C→T 37.4% A57V (GCG→GTG)  glgC → glucose‑1‑phosphate adenylyltransferase
RA 2,677,626 C→T 40.6% A102T (GCA→ACA)  yhgA ← predicted transposase
RA 2,687,159 G→A 44.0% L374L (CTG→TTG)  pck ← phosphoenolpyruvate carboxykinase
RA 2,702,622 G→T 5.1% V5F (GTC→TTC)  aroB → 3‑dehydroquinate synthase
RA 2,732,305:1 +G 40.4% coding (375/723 nt) yheO → predicted DNA‑binding transcriptional regulator
RA 2,732,642 C→A 49.0% Q238K (CAA→AAA)  yheO → predicted DNA‑binding transcriptional regulator
RA 2,850,177 G→A 54.2% Q286* (CAG→TAG)  yjeP ← predicted mechanosensitive channel
RA 2,858,845 G→A 48.0% S373S (TCG→TCA amiB → N‑acetylmuramoyl‑l‑alanine amidase II
RA 2,864,584 C→T 43.2% A295A (GCC→GCT hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 2,912,429 C→T 47.8% A135T (GCG→ACG)  pyrI ← aspartate carbamoyltransferase, regulatory subunit
RA 2,947,335 A→G 53.7% L158P (CTG→CCG)  dnaT ← DNA biosynthesis protein
RA 2,948,123 T→C 33.8% T40A (ACC→GCC)  yjjB ← conserved inner membrane protein
RA 2,971,396 T→C 28.4% V71A (GTG→GCG)  serB → 3‑phosphoserine phosphatase
RA 2,986,542 A→G 33.1% L19L (TTG→CTG)  arcA ← DNA‑binding response regulator in two‑component regulatory system with ArcB or CpxA
RA 2,987,375 A→G 55.8% A48A (GCA→GCG yjtD → predicted rRNA methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ minE 899417 899451 35 11 [10] [6] 12 pspD peripheral inner membrane phage‑shock protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE 5862 =60 (0.950)33 (0.580)
+TAGTG
4/90 NT 36.4% coding (598/777 nt) yaaA conserved hypothetical protein
?minE 904500 = 68 (1.070)coding (471/843 nt) ycjP predicted sugar transporter subunit
* ? minE = 13961868 (1.070)58 (1.210)
+GTTTTGGTTTCG
5/76 NT 54.2% coding (549/2598 nt) htrE predicted outer membrane usher protein
?minE = 389119 61 (0.960)coding (637/1161 nt) ybdL methionine aminotransferase, PLP‑dependent
* ? minE = 20848398 (1.550)7 (0.120) 3/94 NT 6.9% coding (493/804 nt) dkgB 2,5‑diketo‑D‑gluconate reductase B
?minE = 208508 98 (1.650)coding (518/804 nt) dkgB 2,5‑diketo‑D‑gluconate reductase B
* ? minE 344370 =80 (1.260)49 (1.020)
+CCTTTTTCGGTG
5/76 NT 40.8% intergenic (‑128/‑18) ybbK/ybbL predicted protease, membrane anchored/predicted transporter subunit
?minE 2323857 = 107 (1.690)coding (497/963 nt) pfkA 6‑phosphofructokinase I
* ? minE 978654 =51 (0.810)12 (0.230)
+TGACACAC
3/84 NT 19.6% coding (491/2046 nt) dcp dipeptidyl carboxypeptidase II
?minE = 1179011 66 (1.040)coding (461/984 nt) ansA cytoplasmic L‑asparaginase I
* ? minE = 127140349 (0.770)10 (0.180) 3/88 NT 19.2% coding (854/1695 nt) yedQ predicted diguanylate cyclase
?minE = 1271414 41 (0.740)coding (843/1695 nt) yedQ predicted diguanylate cyclase
* ? minE 1515502 =57 (0.900)5 (0.090) 3/90 NT 8.9% intergenic (‑12/+25) purF/cvpA amidophosphoribosyltransferase/membrane protein required for colicin V production
?minE 1515555 = 51 (0.890)coding (461/489 nt) cvpA membrane protein required for colicin V production
* ? minE = 155796080 (1.260)6 (0.100) 3/92 NT 8.2% coding (1000/1164 nt) emrK EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
?minE = 1557988 61 (1.050)coding (972/1164 nt) emrK EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
* ? minE = 157475149 (0.770)7 (0.130) 3/86 NT 13.1% coding (785/885 nt) xapR DNA‑binding transcriptional activator
?minE = 1574788 51 (0.940)coding (748/885 nt) xapR DNA‑binding transcriptional activator
* ? minE 1597441 =41 (0.650)83 (1.720)
+CAACTAAACGAG
8/76 NT 65.5% coding (241/792 nt) ucpA predicted oxidoredutase, sulfate metabolism protein
?minE 2092379 = 74 (1.170)coding (771/1269 nt) yhaO predicted transporter
* ? minE = 16117561 (0.020)39 (0.630)
+A
11/98 NT 62.4% coding (1397/1404 nt) eutA reactivating factor for ethanolamine ammonia lyase
?minE 2083040 = 47 (0.740)coding (66/663 nt) yqjA conserved inner membrane protein
* ? minE 1706121 =82 (1.290)9 (0.160) 4/88 NT 12.2% coding (162/495 nt) yphA predicted inner membrane protein
?minE 1706138 = 57 (1.020)coding (179/495 nt) yphA predicted inner membrane protein
* ? minE = 199326966 (1.040)8 (0.150) 3/84 NT 10.8% coding (562/1257 nt) nupG nucleoside transporter
?minE = 1993315 77 (1.450)coding (608/1257 nt) nupG nucleoside transporter
* ? minE = 212088199 (1.560)7 (0.120) 3/94 NT 7.1% intergenic (+5/‑15) yraN/yraO conserved hypothetical protein/DnaA initiator‑associating factor for replication initiation
?minE = 2120914 91 (1.530)coding (19/591 nt) yraO DnaA initiator‑associating factor for replication initiation
* ? minE = 268019790 (1.420)6 (0.100) 3/92 NT 6.7% coding (493/2322 nt) feoB fused ferrous iron transporter, protein B
?minE = 2680215 83 (1.420)coding (475/2322 nt) feoB fused ferrous iron transporter, protein B
* ? minE 2738325 =86 (1.360)6 (0.140) 3/70 NT 11.1% intergenic (+99/‑430) tufA/rpsJ protein chain elongation factor EF‑Tu/30S ribosomal subunit protein S10
?minE 2738371 = 36 (0.810)intergenic (+145/‑384) tufA/rpsJ protein chain elongation factor EF‑Tu/30S ribosomal subunit protein S10