Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R2
|
44 |
10.9 |
588504 |
78.3% |
460798 |
57.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,858,035 |
C→A |
100% |
T103T (ACC→ACA) |
amiB → |
N‑acetylmuramoyl‑l‑alanine amidase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,858,035 | 0 | C | A | 100.0%
| 10.6
/ NA
| 5 | T103T (ACC→ACA) | amiB | N‑acetylmuramoyl‑l‑alanine amidase II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGG > minE/2858012‑2858073
|
cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:411681/62‑1 (MQ=255)
cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:430701/62‑1 (MQ=255)
cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:509349/62‑1 (MQ=255)
cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:556210/62‑1 (MQ=255)
tACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:216120/61‑1 (MQ=255)
|
CTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGG > minE/2858012‑2858073
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
