Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R2
|
230 |
93.6 |
4701257 |
96.3% |
4527310 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,136,538 |
T→C |
39.7% |
K129K (AAA→AAG) |
yniB ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,136,538 | 0 | T | C | 39.7%
| 40.6
/ 75.6
| 68 | K129K (AAA→AAG) | yniB | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (17/24); new base C (13/14); total (30/38) |
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 |
GAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGG > minE/1136472‑1136608
|
gAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg > 1:4333386/1‑71 (MQ=255)
gAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTgg < 1:830604/71‑1 (MQ=255)
aaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTGACGCGTTAGCCCCTCTTCCCCTTTGGCt < 1:2514627/71‑1 (MQ=255)
aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCtct < 1:1839950/71‑3 (MQ=255)
aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCATCTTCCCCCTTGGCttt < 1:3430574/71‑1 (MQ=255)
gTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCttttt < 1:1808952/70‑1 (MQ=255)
tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCttttt < 1:3788931/69‑1 (MQ=255)
tGGTGCGGAACAACAATACGGGACTCAATTTGTTCCCGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTc > 1:2274411/1‑68 (MQ=255)
tGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAg < 1:331186/71‑1 (MQ=255)
ggTGCGGAACAACAATACGGGACTCAATTTGTTCACGGGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg < 1:1303004/70‑1 (MQ=255)
ggTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAg < 1:1880778/70‑1 (MQ=255)
cGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCa > 1:2128084/1‑65 (MQ=255)
ggAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCTCCCTTGGCTTTTTCCAGAATGa < 1:68959/70‑1 (MQ=255)
aacaacAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAg > 1:3182386/1‑69 (MQ=255)
acaacaATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg < 1:442104/71‑1 (MQ=255)
caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCtttt > 1:4681897/1‑53 (MQ=255)
caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt > 1:2045915/1‑71 (MQ=255)
caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt > 1:4212213/1‑71 (MQ=255)
aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGaa > 1:4642585/1‑59 (MQ=255)
aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:1004719/71‑1 (MQ=255)
aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:3517158/71‑1 (MQ=255)
aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:2106881/71‑1 (MQ=255)
tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:3407409/70‑1 (MQ=255)
tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:363458/1‑57 (MQ=255)
tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:2176401/1‑57 (MQ=255)
tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:383360/1‑57 (MQ=255)
tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCa < 1:2036884/71‑1 (MQ=255)
gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCaa < 1:4247959/69‑1 (MQ=255)
gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATCAGTTGATCTTCaa < 1:2479995/69‑1 (MQ=255)
ggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGAt < 1:4637335/62‑1 (MQ=255)
aCTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCt < 1:2921203/71‑1 (MQ=255)
tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGc < 1:1080943/38‑1 (MQ=255)
tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGc < 1:330124/38‑1 (MQ=255)
tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg < 1:2762621/47‑1 (MQ=255)
tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg < 1:3483676/56‑1 (MQ=255)
tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTc > 1:4565706/1‑71 (MQ=255)
cAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc > 1:3145928/1‑71 (MQ=255)
cAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTc > 1:1039764/1‑70 (MQ=255)
aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg < 1:1954897/54‑1 (MQ=255)
aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCaa > 1:1238011/1‑62 (MQ=255)
aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc > 1:2720687/1‑70 (MQ=255)
aaTTTGTTCACGCGTTAGCCCCTCTTCCCCCTTgg > 1:4567393/1‑35 (MQ=255)
tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg < 1:2491188/43‑1 (MQ=255)
ttGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc > 1:3355728/1‑70 (MQ=255)
ttGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc > 1:2154554/1‑70 (MQ=255)
ttCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaag < 1:360923/70‑1 (MQ=255)
tctcgcgTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCagga < 1:95016/67‑1 (MQ=255)
cACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:1596760/1‑39 (MQ=255)
cACGCGTTAGCCACTCTTCCCCTTTGGCTTTTTCCAGaa > 1:3966245/1‑39 (MQ=255)
aCGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAAt < 1:3509533/39‑1 (MQ=255)
aCGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAAt < 1:2438034/39‑1 (MQ=255)
gcgTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATg > 1:3661442/1‑38 (MQ=255)
gcgTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATc > 1:2178903/1‑46 (MQ=255)
gTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc > 1:824442/1‑71 (MQ=255)
cccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCTCAAGAAGCGCGCCTGGCg < 1:2551347/71‑1 (MQ=255)
cccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCg < 1:17194/71‑1 (MQ=255)
ctctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc > 1:9170/1‑71 (MQ=255)
ctctTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCt < 1:1621011/46‑1 (MQ=255)
ctctTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc > 1:3963412/1‑71 (MQ=255)
tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt < 1:1032232/36‑1 (MQ=255)
tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:847534/38‑1 (MQ=255)
tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc > 1:2257440/1‑70 (MQ=255)
ctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc > 1:566958/1‑61 (MQ=255)
ctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc > 1:871690/1‑61 (MQ=255)
ttCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCt > 1:2327503/1‑43 (MQ=255)
tCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaag < 1:2201357/51‑1 (MQ=255)
ccccTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCAt < 1:144220/70‑1 (MQ=255)
ccccTTTGGCTTTTTCCAGAATGAGTTAATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCAt < 1:3725476/70‑1 (MQ=255)
tttGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCggg > 1:1043158/1‑71 (MQ=255)
|
GAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGG > minE/1136472‑1136608
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
