Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
978,430 |
A→G |
26.5% |
E561E (GAA→GAG) |
mukB → |
fused chromosome partitioning proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 978,430 | 0 | A | G | 26.5%
| 53.6
/ 21.7
| 34 | E561E (GAA→GAG) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base A (12/13); new base G (4/5); total (16/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATGG > W3110S.gb/978381‑978491
|
gTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCctct > 1:1092460/1‑71 (MQ=255)
gTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCctct > 1:1644578/1‑71 (MQ=255)
gTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCctc > 1:329053/1‑70 (MQ=255)
atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:519522/71‑1 (MQ=255)
atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:858278/71‑1 (MQ=255)
atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:242406/71‑1 (MQ=255)
atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:259917/71‑1 (MQ=255)
atatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:314359/71‑1 (MQ=255)
tatCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCt < 1:50942/70‑1 (MQ=255)
acgaACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGGGTCt < 1:1307504/65‑1 (MQ=255)
gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:143709/71‑1 (MQ=255)
gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:649601/71‑1 (MQ=255)
gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:838034/71‑1 (MQ=255)
gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:517053/71‑1 (MQ=255)
gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:932633/71‑1 (MQ=255)
gCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAgcg < 1:1014965/71‑1 (MQ=255)
cTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCAt > 1:838896/1‑71 (MQ=255)
cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:1307752/70‑1 (MQ=255)
cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:739023/70‑1 (MQ=255)
cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:987139/70‑1 (MQ=255)
cATCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgg < 1:1532135/70‑1 (MQ=255)
aTCAGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATgt < 1:426534/69‑2 (MQ=255)
aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:196792/1‑63 (MQ=255)
aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:1681708/1‑63 (MQ=255)
aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:144365/1‑63 (MQ=255)
aGGAGCTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCa > 1:752886/1‑63 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:1402878/1‑41 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:541891/1‑41 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:457310/1‑41 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:227754/1‑41 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:1853221/1‑41 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCgtg > 1:1546712/1‑41 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCg > 1:801269/1‑39 (MQ=255)
aGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGAgtg > 1:999756/1‑41 (MQ=255)
|
GTCAGGGCAAGAATTTTGATATCGACGAACTGGAAGCCCTGCATCAGGAACTGGAAGCACGCATTGCCTCTCTTTCCGATAGCGTGTCTAACGCCCGTGAAGAGCGCATGG > W3110S.gb/978381‑978491
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
