Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,343,341 |
T→C |
28.9% |
I36T (ATC→ACC) |
yhbN → |
predicted transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,343,341 | 0 | T | C | 28.9%
| 61.8
/ 33.8
| 45 | I36T (ATC→ACC) | yhbN | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (23/9); new base C (4/9); total (27/18) |
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 |
CAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGT > W3110S.gb/3343279‑3343402
|
cAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg < 1:903162/71‑1 (MQ=255)
cAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg < 1:394872/71‑1 (MQ=255)
cAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg < 1:369368/71‑1 (MQ=255)
cAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg < 1:1582436/71‑1 (MQ=255)
aGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGa < 1:679145/71‑1 (MQ=255)
aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGa < 1:846290/71‑1 (MQ=255)
aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGa < 1:1434271/71‑1 (MQ=255)
aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGa < 1:1423239/71‑1 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGAt > 1:914242/1‑38 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:537446/1‑71 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:391446/1‑71 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:370991/1‑71 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:1355037/1‑71 (MQ=255)
tttGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAgg > 1:1359921/1‑71 (MQ=255)
tttGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAgg > 1:777564/1‑71 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:693251/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:20369/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:249517/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:33555/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:1076759/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:36785/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:370992/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:1356384/1‑70 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAgg > 1:784365/1‑69 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:871673/1‑70 (MQ=255)
ccGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAAt < 1:1784715/45‑1 (MQ=255)
cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:275653/1‑71 (MQ=255)
cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:315344/1‑71 (MQ=255)
cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:1203905/1‑71 (MQ=255)
cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:631406/1‑71 (MQ=255)
cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc > 1:1631772/1‑70 (MQ=255)
cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc > 1:177671/1‑70 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCgg > 1:1751991/1‑71 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCgg > 1:1377853/1‑71 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCgg > 1:598947/1‑71 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCgg > 1:664537/1‑71 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCgg > 1:1663353/1‑71 (MQ=255)
aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:770782/71‑1 (MQ=255)
aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:383340/71‑1 (MQ=255)
aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:1464745/71‑1 (MQ=255)
aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:431634/71‑1 (MQ=255)
aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:366884/71‑1 (MQ=255)
aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:358572/71‑1 (MQ=255)
aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:133183/71‑1 (MQ=255)
aTCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:115305/71‑1 (MQ=255)
tCAGACGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:1325947/70‑1 (MQ=255)
|
CAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGT > W3110S.gb/3343279‑3343402
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
