Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,343,341 |
T→C |
26.1% |
I36T (ATC→ACC) |
yhbN → |
predicted transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,343,341 | 0 | T | C | 26.1%
| 115.3
/ 48.2
| 69 | I36T (ATC→ACC) | yhbN | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (21/30); new base C (10/8); total (31/38) |
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC > W3110S.gb/3343282‑3343408
|
cTCACTTCTGGCCGCCCGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGa < 1:467265/69‑1 (MQ=255)
tCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGaa < 1:2152166/69‑1 (MQ=255)
tCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg > 1:2141446/1‑68 (MQ=255)
tCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg > 1:1050030/1‑68 (MQ=255)
cTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGCCCCACATTGAATCgg > 1:183410/1‑67 (MQ=255)
cTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCgg > 1:2234646/1‑67 (MQ=255)
tGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGAcc > 1:1143624/1‑69 (MQ=255)
tGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGAcc > 1:1855208/1‑69 (MQ=255)
cgccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCa < 1:3258587/69‑1 (MQ=255)
aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca > 1:3283469/1‑47 (MQ=255)
aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca > 1:2808835/1‑47 (MQ=255)
aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca > 1:3642300/1‑47 (MQ=255)
tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg < 1:31256/53‑1 (MQ=255)
tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg < 1:2450187/53‑1 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat > 1:1416615/1‑69 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat > 1:867581/1‑69 (MQ=255)
ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGat > 1:37162/1‑69 (MQ=255)
cATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:1476464/1‑67 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGa > 1:34397/1‑39 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:3338426/1‑43 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:3194954/1‑43 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:2465797/1‑43 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:2466474/1‑43 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:947783/1‑68 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:1391626/1‑68 (MQ=255)
tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:1987831/1‑68 (MQ=255)
gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTg > 1:450745/1‑55 (MQ=255)
gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCaa < 1:3023920/68‑1 (MQ=255)
gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACAAGCAAtct > 1:1677662/1‑51 (MQ=255)
cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:2063728/68‑1 (MQ=255)
cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:2561274/68‑1 (MQ=255)
cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:1162525/68‑1 (MQ=255)
cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:642105/68‑1 (MQ=255)
cGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGt < 1:1700767/69‑1 (MQ=255)
ggAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGtt < 1:3677516/69‑1 (MQ=255)
ggAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGtt < 1:644465/69‑1 (MQ=255)
ggAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGtt < 1:1870891/69‑1 (MQ=255)
agaCACTGATCAGCCTATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1638503/69‑1 (MQ=255)
agaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1631591/69‑1 (MQ=255)
gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc < 1:1858571/69‑1 (MQ=255)
gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc < 1:1901491/69‑1 (MQ=255)
gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1635776/68‑1 (MQ=255)
gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1527876/68‑1 (MQ=255)
acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc > 1:2536496/1‑68 (MQ=255)
acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc > 1:3330352/1‑68 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCaa > 1:2254343/1‑36 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCaa > 1:3636722/1‑36 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:723998/45‑1 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:568683/45‑1 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:1362960/45‑1 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:3685169/45‑1 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:2081066/45‑1 (MQ=255)
acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCttt < 1:3012187/69‑1 (MQ=255)
cTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCttt < 1:2043389/68‑1 (MQ=255)
gATCAGCCGATCCACATTGAATCGGCCCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:758944/1‑69 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc < 1:297941/51‑1 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:3672433/1‑69 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc > 1:2768357/1‑68 (MQ=255)
gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTT‑ATATGCAAGGCAACGTGGTTACCTTTAc > 1:1325264/1‑67 (MQ=255)
cAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:3348527/69‑1 (MQ=255)
ccGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAAt < 1:3668280/69‑1 (MQ=255)
cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:3446294/49‑1 (MQ=255)
cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:703188/49‑1 (MQ=255)
cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:2700261/49‑1 (MQ=255)
cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:975818/49‑1 (MQ=255)
aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:566460/69‑1 (MQ=255)
aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:3307631/69‑1 (MQ=255)
aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:281880/69‑1 (MQ=255)
aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:2692158/69‑1 (MQ=255)
|
CTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC > W3110S.gb/3343282‑3343408
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
