breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 9,442 T→C 28.3% L46S (TTA→TCA)  mog → predicted molybdochelatase
RA 15,286 C→T 61.5% D373D (GAC→GAT dnaJ → chaperone Hsp40, co‑chaperone with DnaK
RA 45,813 C→T 78.6% P3S (CCG→TCG)  yaaU → predicted transporter
RA 47,828 A→G 76.6% V20V (GTA→GTG kefC → potassium:proton antiporter
RA 65,135 C→T 51.2% D216N (GAT→AAT)  polB ← DNA polymerase II
RA 70,244 T→C 34.2% intergenic (‑196/‑143) araB ← / → araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
RA 70,434 C→T 47.5% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator
RA 70,581 C→T 57.7% V65V (GTC→GTT araC → DNA‑binding transcriptional dual regulator
RA 70,740 T→G 49.1% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator
RA 71,079 C→T 59.8% R231R (CGC→CGT araC → DNA‑binding transcriptional dual regulator
RA 71,082 T→C 59.8% I232I (ATT→ATC araC → DNA‑binding transcriptional dual regulator
RA 71,085 T→C 57.8% S233S (AGT→AGC araC → DNA‑binding transcriptional dual regulator
RA 71,109 T→C 47.8% T241T (ACT→ACC araC → DNA‑binding transcriptional dual regulator
RA 71,175 A→G 65.9% R263R (CGA→CGG araC → DNA‑binding transcriptional dual regulator
RA 71,214 T→C 42.1% F276F (TTT→TTC araC → DNA‑binding transcriptional dual regulator
RA 85,966 T→C 53.8% F113L (TTT→CTT)  ilvI → acetolactate synthase III, large subunit
RA 155,463 A→T 100% *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 158,626 G→A 50.0% S167S (AGC→AGT pcnB ← poly(A) polymerase I
RA 172,490 A→G 41.4% E343E (GAA→GAG fhuB → fused subunits of iron‑hydroxamate transporter and membrane components of ABC superfamily transporter
RA 230,040 C→A 100% R281L (CGC→CTC)  yafC ← predicted DNA‑binding transcriptional regulator
RA 234,234 C→T 65.1% L183L (TTG→TTA gloB ← predicted hydroxyacylglutathione hydrolase
RA 236,101 A→C 54.1% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 236,107 A→C 54.1% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 264,394 T→C 11.5% N13D (AAC→GAC)  yafX ← hypothetical protein
RA 344,381 G→A 72.4% intergenic (+166/‑247) yahL → / → yahM hypothetical protein/hypothetical protein
RA 381,851 A→C 57.8% G88G (GGT→GGG yaiF ← hypothetical protein
RA 393,168 T→C 79.2% G310G (GGT→GGC yaiU → hypothetical protein
RA 408,996 G→A 39.1% A83V (GCG→GTG)  rdgC ← DNA‑binding protein, non‑specific
RA 419,576 G→A 20.8% A254A (GCG→GCA brnQ → predicted branched chain amino acid transporter
RA 470,819 A→G 55.7% E320E (GAA→GAG mdlB → fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily
RA 487,168 C→T 23.8% P470L (CCA→CTA)  kefA → fused mechanosensitive channel proteins
RA 488,522 A→T 23.2% V921V (GTA→GTT kefA → fused mechanosensitive channel proteins
RA 488,526 C→T 23.2% L923F (CTT→TTT)  kefA → fused mechanosensitive channel proteins
RA 488,534 T→C 23.9% F925F (TTT→TTC kefA → fused mechanosensitive channel proteins
RA 495,162 G→A 21.7% E273E (GAG→GAA htpG → molecular chaperone HSP90 family
RA 496,170 A→G 45.9% E609E (GAA→GAG htpG → molecular chaperone HSP90 family
RA 505,179 C→T 34.6% L348L (CTG→TTG)  ushA → bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase
RA 515,934 C→T 100% A43V (GCG→GTG)  ybbM → predicted inner membrane protein
RA 531,446 C→T 62.7% intergenic (‑1/‑229) ybbS ← / → allA transcriptional activator of the allD operon/ureidoglycolate hydrolase
RA 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 579,680 G→A 100% intergenic (+371/‑377) ybcW → / → nohB hypothetical protein/DNA packaging protein
RA 588,045 A→G 27.6% D711D (GAT→GAC nfrA ← bacteriophage N4 receptor, outer membrane subunit
RA 614,426 C→T 68.6% L349L (CTC→CTT entF → enterobactin synthase multienzyme complex component, ATP‑dependent
MC JC 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 669,029 C→T 30.8% E37K (GAA→AAA)  ybeA ← conserved hypothetical protein
RA 798,003 C→T 11.1% A343V (GCG→GTG)  modC → molybdate transporter subunit
RA 810,589 T→G 84.1% M275R (ATG→AGG)  bioB → biotin synthase
RA 827,597 C→T 82.1% P26P (CCG→CCA ybhS ← predicted transporter subunit
RA 847,104 A→G 34.6% R146R (CGT→CGC glnP ← glutamine transporter subunit
RA 887,947 G→A 23.7% A35T (GCT→ACT)  ybjK → predicted DNA‑binding transcriptional regulator
RA 904,996 G→A 70.5% intergenic (‑107/‑19) ybjP ← / → ybjQ predicted lipoprotein/conserved hypothetical protein
RA 906,962 G→A 100% H72Y (CAC→TAC)  ybjS ← predicted NAD(P)H oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 913,779 A→G 68.1% L153P (CTG→CCG)  hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 938,015 A→G 14.1% Q75Q (CAA→CAG lolA → chaperone for lipoproteins
RA 1,006,848 A→T 24.3% K159* (AAA→TAA)  ycbW → hypothetical protein
RA 1,016,291 T→C 50.0% V52A (GTA→GCA)  rmf → ribosome modulation factor
RA 1,020,867 A→G 57.6% S159P (TCC→CCC)  sulA ← SOS cell division inhibitor
RA 1,032,491 C→T 100% intergenic (‑357/‑70) serT ← / → hyaA tRNA‑Ser/hydrogenase 1, small subunit
RA 1,051,238 T→C 22.8% intergenic (+286/+147) insB → / ← cspH IS1 transposase InsAB'/stress protein, member of the CspA‑family
RA 1,056,565 G→A 58.5% G12G (GGC→GGT torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,068,568 T→A 24.0% G22G (GGT→GGA ymdF → conserved hypothetical protein
RA 1,075,828 T→C 67.3% L1159L (TTA→TTG putA ← fused DNA‑binding transcriptional regulator, proline dehydrogenase, and pyrroline‑5‑carboxylate dehydrogenase
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,101,111 G→A 30.0% S272L (TCG→TTG)  csgG ← outer membrane lipoprotein
RA 1,109,964 G→A 100% C185C (TGC→TGT mdoC ← membrane protein required for modification of periplasmic glucan
RA 1,172,268 T→C 48.3% K1092E (AAG→GAG)  mfd ← transcription‑repair coupling factor
RA 1,175,236 C→A 66.6% Q102H (CAG→CAT mfd ← transcription‑repair coupling factor
RA 1,181,288 T→G 76.7% D64E (GAT→GAG cobB → deacetylase of acetyl‑CoA synthetase, NAD‑dependent
RA 1,185,915 A→G 39.4% M36T (ATG→ACG)  potB ← polyamine transporter subunit
RA 1,207,066 A→G 13.7% E440E (GAA→GAG ymfN → predicted DNA‑binding transcriptional regulator
RA 1,210,013 T→C 10.3% L102P (CTG→CCG)  ymfS → hypothetical protein
RA 1,244,320 C→T 100% C113Y (TGC→TAC)  ldcA ← L,D‑carboxypeptidase A
RA 1,282,409 A→G 15.2% P323P (CCA→CCG narG → nitrate reductase 1, alpha subunit
RA 1,290,774 A→G 32.0% intergenic (‑65/‑9) ychJ ← / → rssA conserved hypothetical protein/conserved hypothetical protein
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
RA 1,339,708 G→A 100% A722T (GCC→ACC)  acnA → aconitate hydratase 1
RA 1,352,814 G→A 18.0% intergenic (‑61/+307) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein
RA 1,373,368 G→A 86.1% R480H (CGT→CAT)  ycjM → predicted glucosyltransferase
RA 1,376,428 G→A 100% V209M (GTG→ATG)  ycjP → predicted sugar transporter subunit
RA 1,400,571 G→A 14.6% L224L (CTG→TTG)  fnr ← DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth
RA 1,426,674 T→C 71.2% D393D (GAT→GAC trkG → potassium transporter subunit
RA 1,427,305 T→C 34.8% V72A (GTT→GCT)  ynaK → conserved hypothetical protein
RA 1,427,990 G→A 29.1% intergenic (+296/‑178) ydaY → / → ynaA hypothetical protein/predicted tail protein
RA 1,441,198 G→A 15.2% T434I (ACC→ATC)  ydbK ← fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase
RA 1,463,502 C→T 73.9% P299L (CCG→CTG)  paaJ → predicted beta‑ketoadipyl CoA thiolase
RA 1,477,935 G→A 81.0% E360K (GAA→AAA)  ydbD → hypothetical protein
RA 1,495,966:1 +T 73.2% coding (820/924 nt) ydcI ← predicted DNA‑binding transcriptional regulator
RA 1,496,439 A→G 10.5% L116S (TTA→TCA)  ydcI ← predicted DNA‑binding transcriptional regulator
RA 1,521,063 C→T 67.7% S108F (TCC→TTC)  yncB → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
RA 1,533,715 T→C 26.7% G62G (GGT→GGC ydcC → conserved hypothetical protein
RA 1,547,470 C→T 64.1% S91S (TCG→TCA yddL ← predicted lipoprotein
RA 1,569,965 T→C 62.8% Q191Q (CAA→CAG yddW ← predicted liprotein
RA 1,581,839 C→T 57.7% M222I (ATG→ATA ydeM ← conserved hypothetical protein
RA 1,588,010 T→G 61.5% D64A (GAC→GCC)  ydeP ← predicted oxidoreductase
RA 1,595,724 G→A 44.4% pseudogene (56/1401 nt) ydeU ← ECK1502:JW1502:b1509; conserved hypothetical protein
RA 1,606,841 C→T 48.3% A26V (GCC→GTC)  lsrB → AI2 transporter
RA 1,620,710 C→T 83.8% intergenic (‑88/‑124) marC ← / → marR predicted transporter/DNA‑binding transcriptional repressor
RA 1,657,562 T→C 54.4% I14T (ATT→ACT)  ynfB → hypothetical protein
RA 1,682,586 A→G 63.7% Q439R (CAG→CGG)  ydgI → predicted arginine/ornithine antiporter transporter
RA 1,692,839 G→A 77.4% G425D (GGT→GAT)  ydgA → conserved hypothetical protein
RA 1,742,822 A→G 71.2% S400G (AGC→GGC)  ydhC → predicted transporter
RA 1,761,776 C→T 71.9% L154L (CTG→CTA sufS ← selenocysteine lyase, PLP‑dependent
RA 1,775,080 T→C 73.3% F285F (TTT→TTC ydiN → predicted transporter
RA 1,811,850 G→A 32.3% intergenic (+88/‑75) yniC → / → ydjM predicted hydrolase/predicted inner membrane protein regulated by LexA
RA 1,832,108 C→T 22.8% T124T (ACG→ACA astA ← arginine succinyltransferase
RA 1,835,870 C→T 22.9% A14V (GCT→GTT)  ydjY → hypothetical protein
RA 1,838,873 C→T 22.2% P363S (CCG→TCG)  ynjB → conserved hypothetical protein
RA 1,849,128 G→T 16.2% Q199K (CAG→AAG)  selD ← selenophosphate synthase
RA 1,887,874 T→C 75.0% F106L (TTT→CTT)  yeaX → predicted oxidoreductase
RA 1,927,287 T→C 78.0% L45P (CTT→CCT)  yobB → conserved hypothetical protein
RA 1,934,313 G→A 19.0% A53V (GCA→GTA)  eda ← multifunctional 2‑keto‑3‑deoxygluconate 6‑phosphate aldolase, 2‑keto‑4‑hydroxyglutarate aldolase, and oxaloacetate decarboxylase
RA 1,965,064 G→A 52.8% I567I (ATC→ATT flhA ← predicted flagellar export pore protein
RA 1,995,272 T→C 69.2% S524S (TCA→TCG uvrC ← excinuclease UvrABC, endonuclease subunit
RA 1,999,934 A→G 48.7% V6V (GTT→GTC yecC ← predicted transporter subunit
RA 2,000,742 C→T 13.1% M292I (ATG→ATA yedO ← D‑cysteine desulfhydrase, PLP‑dependent
RA 2,069,625 C→T 10.4% intergenic (‑167/‑1147) insH ← / → yoeA IS5 transposase and trans‑activator/CP4‑44 prophage region; ECK1989:JW5326:b1995; predicted disrupted hemin or colicin receptor
RA 2,074,177 G→A 29.8% V168I (GTC→ATC)  flu → antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,077,980 C→T 69.2% Y355Y (TAC→TAT yeeR → predicted membrane protein
RA 2,085,271 A→G 68.2% S127G (AGC→GGC)  sbcB → exonuclease I
RA 2,096,084 G→A 19.3% E160E (GAG→GAA hisB → fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
RA 2,129,117 C→T 40.0% A71T (GCC→ACC)  fcl ← bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase and GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase
RA 2,157,782 T→C 88.3% L128S (TTG→TCG)  mdtB → multidrug efflux system, subunit B
RA 2,160,543 C→T 71.4% F7F (TTC→TTT mdtC → multidrug efflux system, subunit C
RA 2,172,235 C→T 11.6% pseudogene (42/447 nt)
pseudogene (1636/2041 nt)
gatR ←
gatR ←
DNA‑binding transcriptional regulator, N‑ter fragment; ECK2083:JW5340:b2087
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
MC JC 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]
RA 2,198,825 G→A 80.4% A398T (GCG→ACG)  metG → methionyl‑tRNA synthetase
RA 2,264,229 C→T 28.3% L172L (CTG→CTA fruA ← fused fructose‑specific PTS enzyme IIB'BC components
RA 2,276,691 Δ1 bp 100% coding (994/1815 nt) yejA → predicted oligopeptide transporter subunit
RA 2,295,693 C→T 22.2% A18T (GCG→ACG)  ccmH ← heme lyase, CcmH subunit
RA 2,307,310 C→T 33.3% V24V (GTC→GTT eco → ecotin, a serine protease inhibitor
RA 2,313,823 T→C 62.2% E349G (GAA→GGA)  yojL ← predicted thiamine biosynthesis lipoprotein
RA 2,315,568 A→G 51.9% G172G (GGT→GGC ompC ← outer membrane porin protein C
MC JC 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]
RA 2,350,543 G→A 80.0% V337I (GTA→ATA)  nrdA → ribonucleoside diphosphate reductase 1, alpha subunit
RA 2,381,930 Δ1 bp 100% coding (333/759 nt) yfbB ← predicted peptidase
RA 2,397,191 A→G 80.0% F174S (TTC→TCC)  nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M
RA 2,427,890 T→G 100% I246S (ATT→AGT)  yfcH → conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
RA 2,455,159 C→T 9.0% V113I (GTT→ATT)  yfcO ← hypothetical protein
RA 2,501,495 G→A 60.0% S135S (TCG→TCA ddg → palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
RA 2,541,664 G→A 78.9% E129K (GAA→AAA)  crr → glucose‑specific enzyme IIA component of PTS
RA 2,589,664 A→T 57.1% intergenic (+300/‑239) acrD → / → yffB aminoglycoside/multidrug efflux system/conserved hypothetical protein
RA 2,594,104 G→A 53.3% H138Y (CAT→TAT)  ypfI ← predicted hydrolase
RA 2,599,304 G→A 100% M57I (ATG→ATA bcp → thiol peroxidase, thioredoxin‑dependent
RA 2,609,568 T→C 17.5% V69V (GTT→GTC hyfI → hydrogenase 4, Fe‑S subunit
RA 2,636,526 C→T 75.9% L261L (CTG→CTA yfgL ← protein assembly complex, lipoprotein component
RA 2,646,699 C→G 55.6% Q1415H (CAG→CAC yfhM ← conserved hypothetical protein
RA 2,667,558 T→C 86.9% intergenic (‑6/‑130) hcaR ← / → hcaE DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit
RA 2,669,838 T→C 66.1% V92A (GTT→GCT)  hcaC → 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit
RA 2,688,036 T→C 100% I43V (ATT→GTT)  yfhG ← conserved hypothetical protein
RA 2,697,845 G→A 36.1% G144D (GGT→GAT)  yfhH → predicted DNA‑binding transcriptional regulator
RA 2,702,790 C→T 69.4% intergenic (‑71/+201) rnc ← / ← lepB RNase III/leader peptidase
RA 2,706,728 G→A 48.9% P228L (CCA→CTA)  rseB ← anti‑sigma factor
RA 2,710,918 A→T 11.3% V168E (GTG→GAG)  yfiC ← predicted methyltransferase
RA 2,717,229 G→A 59.4% intergenic (‑45/‑162) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2
RA 2,759,336 C→T 65.8% T45I (ACA→ATA)  yfjJ → hypothetical protein
RA 2,773,435 T→C 77.1% L488L (TTG→CTG)  yfjW → predicted inner membrane protein
RA 2,777,419:1 +A 100% coding (3964/4581 nt) ypjA ← adhesin‑like autotransporter
RA 2,794,102 G→A 75.0% L398L (TTG→TTA gabP → gamma‑aminobutyrate transporter
RA 2,802,461 G→A 25.0% A102T (GCC→ACC)  nrdF → ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like
RA 2,809,581 G→A 55.7% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 2,821,480 T→C 59.4% T316A (ACC→GCC)  recA ← DNA strand exchange and recombination protein with protease and nuclease activity
RA 2,821,628 G→A 60.7% G266G (GGC→GGT recA ← DNA strand exchange and recombination protein with protease and nuclease activity
RA 2,840,761 A→G 74.1% E372E (GAA→GAG ascB → cryptic 6‑phospho‑beta‑glucosidase
RA 2,841,727 C→T 54.8% A126T (GCC→ACC)  hycH ← protein required for maturation of hydrogenase 3
RA 2,855,194 A→T 35.5% I90N (ATC→AAC)  ygbA ← hypothetical protein
RA 2,855,731 G→T 27.7% intergenic (‑269/‑18) ygbA ← / → mutS hypothetical protein/methyl‑directed mismatch repair protein
RA 2,855,732 G→A 27.8% intergenic (‑270/‑17) ygbA ← / → mutS hypothetical protein/methyl‑directed mismatch repair protein
RA 2,860,110 G→A 71.7% V9I (GTC→ATC)  ygbJ → predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain
RA 2,866,110 T→A 100% intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
RA 2,866,764 G→A 7.3% Q216* (CAG→TAG)  nlpD ← predicted outer membrane lipoprotein
RA 2,867,123 G→A 69.2% P96L (CCG→CTG)  nlpD ← predicted outer membrane lipoprotein
RA 2,875,612 G→A 28.6% D126N (GAT→AAT)  iap → aminopeptidase in alkaline phosphatase isozyme conversion
RA 2,885,984 C→T 100% intergenic (‑109/+250) ygcB ← / ← cysH conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase
RA 2,893,179 A→C 24.6% I110L (ATT→CTT)  ygcP → predicted anti‑terminator regulatory protein
RA 2,894,340 C→T 23.7% W32* (TGG→TGA ygcQ ← predicted flavoprotein
RA 2,906,931 A→G 46.4% V464V (GTT→GTC pyrG ← CTP synthetase
RA 2,910,539 C→A 76.2% R590L (CGC→CTC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,927,326 G→A 26.4% V148M (GTG→ATG)  sdaC → predicted serine transporter
RA 2,931,037 C→A 42.7% A212A (GCG→GCT fucO ← L‑1,2‑propanediol oxidoreductase
RA 2,938,519 A→G 56.3% I166V (ATC→GTC)  fucR → DNA‑binding transcriptional activator
RA 2,964,217 G→A 22.6% N159N (AAC→AAT lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
RA 3,013,681 G→A 100% V11V (GTC→GTT yqeC ← conserved hypothetical protein
RA 3,061,031 A→G 73.5% Q509R (CAG→CGG)  yliK → methylmalonyl‑CoA mutase
RA 3,065,306 A→G 16.7% V175A (GTT→GCT)  ygfI ← predicted DNA‑binding transcriptional regulator
RA 3,079,041 A→G 52.7% I417I (ATT→ATC tktA ← transketolase 1, thiamin‑binding
RA 3,101,264 G→A 22.4% A82V (GCT→GTT)  yggH ← tRNA (m7G46) methyltransferase, SAM‑dependent
RA 3,125,971 A→G 50.0% V236A (GTT→GCT)  glcD ← glycolate oxidase subunit, FAD‑linked
RA 3,135,166 A→G 68.8% intergenic (‑139/+153) pitB ← / ← gss phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase
RA 3,138,506 C→T 5.8% G39S (GGT→AGT)  hybG ← hydrogenase 2 accessory protein
RA 3,164,775 C→T 63.2% A534T (GCA→ACA)  ygiS ← predicted transporter subunit
RA 3,194,164 T→C 80.9% T416A (ACG→GCG)  rfaE ← fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
RA 3,198,610 A→G 58.3% F338L (TTC→CTC)  ygiF ← predicted adenylate cyclase
RA 3,205,103 A→G 79.5% intergenic (‑191/‑16) ygiP ← / → ttdA predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit
RA 3,219,258 T→C 18.9% F379S (TTC→TCC)  ygjG → putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
RA 3,225,032 Δ1 bp 100% coding (143/1434 nt) ygjI → predicted transporter
RA 3,232,470 A→G 11.8% L111L (TTG→CTG)  ygjM ← predicted DNA‑binding transcriptional regulator
MC JC 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]
RA 3,272,176 T→C 31.5% D146G (GAC→GGC)  garR ← tartronate semialdehyde reductase
RA 3,343,341 T→C 26.1% I36T (ATC→ACC)  yhbN → predicted transporter subunit
RA 3,347,872 C→T 24.5% R18W (CGG→TGG)  npr → phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr)
RA 3,349,295 C→T 45.2% A124T (GCC→ACC)  mtgA ← biosynthetic peptidoglycan transglycosylase
RA 3,358,271 A→G 72.7% K1262R (AAA→AGA)  gltB → glutamate synthase, large subunit
RA 3,365,218 A→G 62.8% pseudogene (179/480 nt)
pseudogene (179/1745 nt)
yhcE →
yhcE →
ECK3207:JW3184:b3217; hypothetical protein, N‑ter fragment
ECK3207:JW3184+JW3187:b4569; hypothetical protein
RA 3,398,077 C→T 22.7% A53T (GCA→ACA)  yhdE ← conserved hypothetical protein
RA 3,404,820 G→A 52.1% intergenic (+507/‑471) yhdH → / → accB predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
RA 3,442,070 A→G 18.7% T497A (ACG→GCG)  thiC → thiamin (pyrimidine moiety) biosynthesis protein
RA 3,447,843 A→G 77.5% V1163V (GTT→GTC rpoC ← RNA polymerase, beta prime subunit
RA 3,518,471 T→C 11.2% intergenic (‑305/‑120) yiiU ← / → glpF conserved hypothetical protein/glycerol facilitator
RA 3,566,303 T→C 100% C8C (TGT→TGC yihR → predicted aldose‑1‑epimerase
RA 3,567,710 T→C 35.0% R102R (CGT→CGC yihQ → alpha‑glucosidase
RA 3,573,836 A→G 80.0% V200A (GTG→GCG)  yihN ← predicted transporter
RA 3,587,198 C→T 83.9% E840K (GAA→AAA)  polA ← fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
RA 3,591,880 C→T 11.7% M201I (ATG→ATA yihF ← conserved hypothetical protein
RA 3,622,543 C→T 72.7% A362A (GCG→GCA metE ← 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 3,634,018 T→C 11.3% V46A (GTA→GCA)  yigF → conserved inner membrane protein
RA 3,682,573 G→A 100% L211L (CTG→TTG)  ilvD ← dihydroxyacid dehydratase
RA 3,705,799 A→G 50.0% E70E (GAA→GAG yieN → fused predicted transcriptional regulators
RA 3,709,115 C→T 17.1% E138E (GAG→GAA asnA ← asparagine synthetase A
RA 3,714,423 C→T 48.1% H61Y (CAC→TAC)  atpI → ATP synthase, membrane‑bound accesory subunit
MC JC 3,742,938 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]
MC JC 3,750,593 Δ1,199 bp 100% insH insH
RA 3,780,496 G→A 34.9% G66R (GGG→AGG)  yidK → predicted transporter
RA 3,783,965 C→T 9.2% G13D (GGC→GAC)  yidI ← predicted inner membrane protein
RA 3,792,107 Δ1 bp 100% coding (315/1320 nt) uhpC → membrane protein regulates uhpT expression
RA 3,803,370 A→C 39.4% L32R (CTT→CGT)  setC ← predicted sugar efflux system
RA 3,815,914 T→C 82.6% N702D (AAT→GAT)  spoT ← bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,824,280 T→C 100% pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T 100% pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA 100% pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A 100% pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C 100% pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,533:1 +G 100% pseudogene (666/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,861,751 T→C 78.9% N423S (AAC→AGC)  lldP ← L‑lactate permease
RA 3,918,430 G→A 33.7% L264L (CTG→TTG)  insK ← IS150 conserved protein InsB
RA 4,007,539 G→A 14.3% pseudogene (367/393 nt) yhiK → ECK3474:JW5674:b3489; hypothetical protein
RA 4,008,583 C→T 30.9% Q253* (CAA→TAA)  yhiJ → hypothetical protein
RA 4,025,948 A→G 26.8% I268T (ATC→ACC)  nikA ← nickel transporter subunit
RA 4,031,980 T→C 46.7% N663D (AAC→GAC)  zntA ← zinc, cobalt and lead efflux system
RA 4,066,786 C→T 66.7% intergenic (+144/‑129) asd → / → glgB aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme
RA 4,103,846 T→C 30.3% Y5H (TAC→CAC)  ompR → DNA‑binding response regulator in two‑component regulatory system with EnvZ
RA 4,124,493 T→C 47.6% intergenic (+95/‑12) damX → / → dam hypothetical protein/DNA adenine methylase
RA 4,154,666 A→G 83.3% I23V (ATT→GTT)  yhfA → conserved hypothetical protein
RA 4,155,146:1 +A 69.4% coding (783/870 nt) prkB ← predicted phosphoribulokinase
RA 4,177,469 G→A 57.3% A9V (GCG→GTG)  gspK ← general secretory pathway component, cryptic
RA 4,182,652 C→T 29.4% V464M (GTG→ATG)  gspD ← general secretory pathway component, cryptic
RA 4,184,035 C→A 26.9% G3* (GGA→TGA) 
R269M (AGG→ATG) 
gspD ←
gspC ←
general secretory pathway component, cryptic
general secretory pathway component, cryptic
RA 4,246,866 T→C 62.5% V81V (GTA→GTG malG ← maltose transporter subunit
RA 4,271,811 A→G 76.9% Y369C (TAT→TGT)  tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,295,565 T→C 69.1% F154F (TTT→TTC nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
MC JC 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,371,273 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,378,355 T→C 53.3% K188E (AAA→GAA)  yjeJ ← hypothetical protein
RA 4,390,267 A→G 12.1% N65D (AAC→GAC)  yjeN → hypothetical protein
RA 4,428,262:1 +G 100% coding (735/855 nt) ulaE → L‑xylulose 5‑phosphate 3‑epimerase
RA 4,486,264 C→T 84.4% P752P (CCG→CCA valS ← valyl‑tRNA synthetase
RA 4,491,067 G→A 66.7% V56V (GTG→GTA yjgP → conserved inner membrane protein
RA 4,501,102 T→C 41.7% noncoding (16/85 nt) leuX → tRNA‑Leu
RA 4,501,500 A→G 83.0% Q23Q (CAA→CAG intB → predicted integrase
RA 4,501,608 A→G 100% K59K (AAA→AAG intB → predicted integrase
RA 4,528,968 A→G 83.0% L242P (CTA→CCA)  yjhH ← predicted lyase/synthase
RA 4,547,549 T→C 17.4% intergenic (+234/‑248) fimE → / → fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
RA 4,549,754 G→A 25.9% intergenic (+43/‑24) fimC → / → fimD chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis
RA 4,549,768 C→T 45.8% intergenic (+57/‑10) fimC → / → fimD chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis
RA 4,563,589 T→A 100% K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase
RA 4,591,162 C→A 15.6% V112V (GTG→GTT hsdR ← endonuclease R
RA 4,615,718 C→T 22.9% intergenic (+33/‑360) prfC → / → osmY peptide chain release factor RF‑3/periplasmic protein
RA 4,640,981 C→T 31.0% A98V (GCT→GTT)  creB → DNA‑binding response regulator in two‑component regulatory system with CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 171663 171706 44 5 [2] [3] 5 fhuB fused subunits of iron‑hydroxamate transporter and membrane components of ABC superfamily transporter
* * ÷ W3110S.gb 283084 283221 138 5 [4] [2] 5 yagF predicted dehydratase
* * ÷ W3110S.gb 284209 284296 88 5 [4] [4] 5 yagF predicted dehydratase
* * ÷ W3110S.gb 422765 422782 18 5 [4] [4] 6 malZ maltodextrin glucosidase
* * ÷ W3110S.gb 492638 492698 61 5 [4] [2] 5 dnaX DNA polymerase III/DNA elongation factor III, tau and gamma subunits
* * ÷ W3110S.gb 573867–575008 575999 992–2133 5 [2] [0] 108 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 598766 598825 60 9 [4] [4] 8 cusA copper/silver efflux system, membrane component
* * ÷ W3110S.gb 721520 721539 20 5 [3] [4] 5 kdpE DNA‑binding response regulator in two‑component regulatory system with KdpD
* * ÷ W3110S.gb 1338219 1338228 10 6 [1] [3] 8 acnA aconitate hydratase 1
* * ÷ W3110S.gb 1352903 1352935–1352914 12–33 6 [4] [3] 5 fabI/ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/conserved hypothetical protein
* * ÷ W3110S.gb 1371186 1371229 44 10 [4] [4] 5 pspD peripheral inner membrane phage‑shock protein
* * ÷ W3110S.gb 1421312 1421328 17 5 [4] [4] 5 ydaG/racR hypothetical protein/predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 2041775 2041807 33 13 [3] [4] 5 yedY predicted reductase
* * ÷ W3110S.gb 2569098 2569131 34 8 [4] [4] 7 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ W3110S.gb 2777816 2777841 26 10 [1] [3] 5 ypjA adhesin‑like autotransporter
* * ÷ W3110S.gb 3799037 3799069 33 5 [4] [4] 9 yicM predicted transporter
* * ÷ W3110S.gb 4213634–4215889 4216705–4215891 3–3072 5 [4] [4] 5 rrlE–[rrfE] rrlE,[rrfE]
* * ÷ W3110S.gb 4401136 4401190 55 5 [4] [4] 7 amiB N‑acetylmuramoyl‑l‑alanine amidase II

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 70064 =28 (0.750)25 (0.970)
+TGGACGCGTACAACT
9/70 NT 39.6% intergenic (‑16/‑323) araB/araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
?W3110S.gb 236044 = 82 (2.210)intergenic (‑42/‑23) rnhA/dnaQ ribonuclease HI, degrades RNA of DNA‑RNA hybrids/DNA polymerase III epsilon subunit
* ? W3110S.gb = 7126532 (0.860)16 (0.600)
+AGGGTCCCTAAGTC
12/72 NT 56.9% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator
?W3110S.gb 3464879 = 2 (0.050)intergenic (‑255/+46) murB/rrfB UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding/5S ribosomal RNA
* ? W3110S.gb = 23679820 (0.540)22 (0.610) 9/98 NT 53.3% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?W3110S.gb 2855720 = 19 (0.520)intergenic (‑258/‑29) ygbA/mutS hypothetical protein/methyl‑directed mismatch repair protein
* ? W3110S.gb = 274373NA (NA)34 (0.920) 15/100 NT 100% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 0 (0.000)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb 314453 =27 (0.730)43 (1.230)
+TCA
20/94 NT 77.2% coding (873/888 nt) eaeH attaching and effacing protein, pathogenesis factor
?W3110S.gb = 3920281 0 (0.000)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb = 315707NA (NA)39 (1.050) 21/100 NT 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb = 48853862 (1.670)18 (0.520)
+GTG
4/94 NT 25.0% coding (2779/3363 nt) kefA fused mechanosensitive channel proteins
?W3110S.gb 3380815 = 53 (1.430)coding (218/1368 nt) degQ serine endoprotease, periplasmic
* ? W3110S.gb 566000 =NA (NA)54 (1.460) 21/100 NT 100% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 0 (0.000)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 713380 =11 (0.300)51 (1.500) 22/92 NT 71.8% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?W3110S.gb = 4125340 30 (0.880)coding (836/837 nt) dam DNA adenine methylase
* ? W3110S.gb = 71395455 (1.480)42 (1.130) 18/100 NT 45.2% coding (546/546 nt) seqA regulatory protein for replication initiation
?W3110S.gb 2809398 = 47 (1.270)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator
* ? W3110S.gb 1209367 =11 (0.300)17 (0.680) 10/68 NT 55.4% coding (290/630 nt) ycfK hypothetical protein
?W3110S.gb 1211196 = 20 (0.800)pseudogene (37/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 120938218 (0.490)15 (0.600) 5/68 NT 48.3% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 20 (0.800)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb 2018571 =0 (0.000)33 (0.950) 17/94 NT 100% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 20185831 (0.030)33 (0.930) 13/96 NT 97.2% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb 2172309 =NA (NA)32 (0.900) 16/96 NT 100% pseudogene (1562/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb 3932930 = 0 (0.000)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2173564NA (NA)59 (1.620) 23/98 NT 100% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 0 (0.000)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173565NA (NA)33 (0.890) 18/100 NT 100% pseudogene (306/2041 nt)
pseudogene (306/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb = 3932933 0 (0.000)coding (222/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2412225NA (NA)34 (0.920) 18/100 NT 85.0% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 6 (0.160)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb = 285574745 (1.210)8 (0.220) 6/98 NT 15.4% intergenic (‑285/‑2) ygbA/mutS hypothetical protein/methyl‑directed mismatch repair protein
?W3110S.gb 4124505 = 44 (1.210)coding (1/837 nt) dam DNA adenine methylase
* ? W3110S.gb 3071050 =38 (1.020)28 (0.950)
+AAATGCTCTC
3/80 NT 48.7% coding (229/1164 nt) pgk phosphoglycerate kinase
?W3110S.gb 4347351 = 36 (0.970)coding (761/1356 nt) melA alpha‑galactosidase, NAD(P)‑binding
* ? W3110S.gb 3748799 =2 (0.050)52 (1.400) 21/100 NT 96.3% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb 4547364 =3 (0.080)11 (0.360) 7/82 NT 77.3% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb 4547678 = 4 (0.130)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? W3110S.gb = 45473723 (0.080)15 (0.500) 11/82 NT 82.3% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb = 4547668 4 (0.130)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin