Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,282,409 |
A→G |
P323P (CCA→CCG) |
narG → |
nitrate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,282,409 | 0 | A | G | 100.0%
| 221.5
/ NA
| 64 | P323P (CCA→CCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/35); total (29/35) |
GGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGT > W3110S.gb/1282341‑1282462
|
ggCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAg > 1:3856177/1‑71 (MQ=255)
gCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGc > 1:69257/1‑71 (MQ=255)
ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAg > 1:691968/1‑71 (MQ=255)
gATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa > 1:1287575/1‑71 (MQ=255)
gATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa > 1:1373675/1‑71 (MQ=255)
aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAt < 1:4458182/71‑1 (MQ=255)
tGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAtt > 1:4006132/1‑71 (MQ=255)
tGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAtt > 1:156013/1‑71 (MQ=255)
tGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAtt > 1:2025657/1‑71 (MQ=255)
gCGGCAATGGCGCTGGCGATGGGCCAGGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAttt > 1:3989143/1‑71 (MQ=255)
gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa > 1:4406519/1‑68 (MQ=255)
gCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAttt > 1:4381139/1‑71 (MQ=255)
aTGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa < 1:3834750/62‑1 (MQ=255)
aTGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCAcc < 1:2182750/69‑1 (MQ=255)
tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc < 1:4582094/71‑1 (MQ=255)
ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:3833289/1‑70 (MQ=255)
ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:870248/1‑70 (MQ=255)
ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:348486/1‑70 (MQ=255)
ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:3385118/1‑70 (MQ=255)
aTGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAg < 1:3332106/48‑1 (MQ=255)
cGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa < 1:1860224/42‑1 (MQ=255)
cGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACAcc > 1:903622/1‑70 (MQ=255)
cGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACAcc > 1:990681/1‑70 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:4153990/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:428211/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:489215/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:1280048/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:1064035/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:1479906/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:2264217/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:1677429/71‑1 (MQ=255)
gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:2600263/71‑1 (MQ=255)
tAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa < 1:1897490/40‑1 (MQ=255)
tAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTa < 1:4086286/40‑1 (MQ=255)
tAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTAtgtg > 1:4028090/1‑56 (MQ=255)
tAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa > 1:135928/1‑70 (MQ=255)
aaTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACa > 1:4586089/1‑71 (MQ=255)
aaTGCTGCGTGAATTCCACCTCGACAACCCGAGACAGTAtt > 1:461481/1‑41 (MQ=38)
aTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCg < 1:517640/46‑1 (MQ=255)
aTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTa < 1:504011/50‑1 (MQ=255)
aTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTAtg < 1:3966003/52‑1 (MQ=255)
aTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTAtg > 1:2399166/1‑52 (MQ=255)
aTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACAt > 1:3485599/1‑71 (MQ=255)
tgctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAc < 1:1713004/68‑1 (MQ=255)
tgctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAc < 1:1813936/68‑1 (MQ=255)
gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:2401528/1‑46 (MQ=255)
gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:2744423/1‑46 (MQ=255)
gctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:1833812/1‑46 (MQ=255)
ctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTa > 1:3914132/1‑59 (MQ=255)
ctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTa > 1:2669144/1‑59 (MQ=255)
aaTTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACa > 1:3532425/1‑60 (MQ=255)
aaTTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTg < 1:3304739/71‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc < 1:4676478/46‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc < 1:4472188/46‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc < 1:208695/46‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc < 1:170412/46‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTc < 1:3331356/46‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:901447/56‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGt < 1:437874/71‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGt < 1:2739120/71‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGt < 1:2846643/71‑1 (MQ=255)
ttCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGt < 1:3074308/71‑1 (MQ=255)
tCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGAt < 1:676999/64‑1 (MQ=255)
gACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc < 1:496545/53‑1 (MQ=255)
|
GGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGT > W3110S.gb/1282341‑1282462
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
