Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,290,774 |
A→G |
intergenic (‑65/‑9) |
ychJ ← / → rssA |
conserved hypothetical protein/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,290,774 | 0 | A | G | 100.0%
| 158.5
/ NA
| 46 | intergenic (‑65/‑9) | ychJ/rssA | conserved hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/25); total (21/25) |
CAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAA > W3110S.gb/1290709‑1290837
|
caAATGATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGc < 1:1696665/71‑1 (MQ=255)
aaaTAATCTCCCCGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCg < 1:613108/71‑1 (MQ=255)
aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGTTAGCGa < 1:1618999/71‑1 (MQ=255)
aataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGa < 1:226892/71‑1 (MQ=255)
ataatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGAt < 1:4454471/71‑1 (MQ=255)
taatCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGAtg > 1:795212/1‑71 (MQ=255)
atCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGAtgtg > 1:642986/1‑71 (MQ=255)
ccTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTAc < 1:675803/71‑1 (MQ=255)
ccTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTAc < 1:682869/71‑1 (MQ=255)
cTGGAAACAATAACGGCGTATTAACCGCCTGAGTATCACTATGTTAACCGAGCGGTAGCGATCTGGCTACg < 1:237012/71‑1 (MQ=255)
tGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGa > 1:3070200/1‑71 (MQ=255)
tGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGa > 1:3961812/1‑71 (MQ=255)
aCAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCa > 1:1341349/1‑71 (MQ=255)
aCAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCa > 1:769423/1‑71 (MQ=255)
tAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTg < 1:1368773/65‑1 (MQ=255)
tAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTcc < 1:1297144/71‑1 (MQ=255)
aCGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAg > 1:1280971/1‑71 (MQ=255)
tATTAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTg < 1:1698757/57‑1 (MQ=255)
ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCt > 1:1253219/1‑71 (MQ=255)
ttAACCGCCTGAGTAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCt > 1:2838060/1‑71 (MQ=255)
tAGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat > 1:1158295/1‑70 (MQ=255)
aGCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat > 1:381929/1‑69 (MQ=255)
gCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAAt > 1:3631605/1‑54 (MQ=255)
gCACTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat > 1:3496625/1‑68 (MQ=255)
aCTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga < 1:3173884/70‑1 (MQ=255)
aCTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgag > 1:2672259/1‑69 (MQ=255)
cTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat < 1:1591503/65‑1 (MQ=255)
cTATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat < 1:628902/65‑1 (MQ=255)
tATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCATGGGAATCTTGCGGGaat < 1:719365/61‑1 (MQ=255)
tATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaataat < 1:3083546/64‑1 (MQ=255)
tATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaat < 1:2684808/61‑1 (MQ=255)
tATGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGaat < 1:3934730/61‑1 (MQ=255)
aTGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga > 1:4594391/1‑67 (MQ=255)
aTGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga > 1:3501096/1‑67 (MQ=255)
aTGTTAACCGAGCGCTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATgaga > 1:2347416/1‑67 (MQ=255)
tGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGa > 1:3069048/1‑70 (MQ=255)
tGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGa > 1:3740615/1‑70 (MQ=255)
tGTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGAt < 1:2314403/71‑1 (MQ=255)
gTTAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGa < 1:224835/69‑1 (MQ=255)
ttAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATa > 1:1678718/1‑70 (MQ=255)
tAACCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATa > 1:2135443/1‑69 (MQ=255)
aCCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaaaa < 1:4016686/71‑1 (MQ=255)
aCCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaaaa < 1:3798920/71‑1 (MQ=255)
aCCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaaaa < 1:2190231/71‑1 (MQ=255)
aCCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaaaa < 1:1073157/71‑1 (MQ=255)
aCCGAGCGGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATaaaa < 1:1005094/70‑1 (MQ=255)
|
CAAATAATCTCCCTGGAAACAATAACGGCGTATTAACCGCCTGAGTAGCACTATGTTAACCGAGCAGTAGCGATGTGGCTACGATTGCATTCCAGGGGAATCTTGCGGGAATAATGAGAAAGATAAAAA > W3110S.gb/1290709‑1290837
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
