Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,236,590 |
G→T |
D127Y (GAT→TAT) |
sanA → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,236,590 | 0 | G | T | 100.0%
| 180.2
/ NA
| 60 | D127Y (GAT→TAT) | sanA | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (30/30); total (30/30) |
GATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTC > W3110S.gb/2236524‑2236655
|
aaTTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTc < 1:2743324/70‑1 (MQ=255)
tttAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCa < 1:2908314/71‑1 (MQ=255)
cGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGc > 1:1149054/1‑71 (MQ=255)
gctgctGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCg < 1:3953840/71‑1 (MQ=255)
gctgctGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCg < 1:4017605/71‑1 (MQ=255)
tgtCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCg < 1:1841569/63‑1 (MQ=255)
gtCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCa > 1:2145237/1‑70 (MQ=255)
atccATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGtt < 1:2135319/69‑1 (MQ=255)
atccATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGtt < 1:2263835/69‑1 (MQ=255)
ccATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGtttt > 1:1550003/1‑71 (MQ=255)
cATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTc < 1:1851090/71‑1 (MQ=255)
cATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTc < 1:4074307/71‑1 (MQ=255)
cATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTc < 1:2288185/71‑1 (MQ=255)
tCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGtttttc > 1:3244838/1‑68 (MQ=255)
tCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGa > 1:1534038/1‑71 (MQ=255)
tCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGa < 1:2353023/71‑1 (MQ=255)
tCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGa > 1:2281702/1‑71 (MQ=255)
ccAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTa < 1:2677296/54‑1 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTaca > 1:1775763/1‑55 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTaca > 1:4082515/1‑55 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCggt > 1:4390163/1‑68 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGAt > 1:3330434/1‑70 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGAt > 1:616429/1‑70 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATa > 1:3843150/1‑71 (MQ=255)
aGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATa > 1:4488552/1‑71 (MQ=255)
gATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGa < 1:1744974/68‑1 (MQ=255)
gATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGa < 1:3704362/68‑1 (MQ=255)
atatTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAg < 1:4584247/60‑1 (MQ=255)
atatTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAg < 1:1399528/60‑1 (MQ=255)
atatTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAg < 1:3269927/60‑1 (MQ=255)
atatTGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGa < 1:1745513/67‑1 (MQ=255)
ttGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTGCATCGTGCGTACACGCAAAGTTTTCGATACTAAt < 1:826569/71‑1 (MQ=255)
ttGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt < 1:535994/71‑1 (MQ=255)
ttGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt < 1:88468/71‑1 (MQ=255)
ttGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt < 1:441548/71‑1 (MQ=255)
ttGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt > 1:157360/1‑71 (MQ=255)
tGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt > 1:1545818/1‑70 (MQ=255)
tGTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt > 1:3552205/1‑70 (MQ=255)
gTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGa > 1:1333084/1‑71 (MQ=255)
gTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGa > 1:3959533/1‑71 (MQ=255)
gTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGa > 1:4601956/1‑71 (MQ=255)
gTTCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGa > 1:1722665/1‑71 (MQ=255)
ttCTCGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt < 1:3313194/68‑1 (MQ=255)
ctcGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGAttt > 1:320958/1‑71 (MQ=255)
ctcGATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGAttt > 1:4601728/1‑71 (MQ=255)
gATTACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCat < 1:791628/71‑1 (MQ=255)
tACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTa > 1:2702640/1‑37 (MQ=255)
tACGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCattat > 1:1846676/1‑71 (MQ=255)
aCGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCattat < 1:1390487/70‑1 (MQ=255)
aCGCAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCattat > 1:673902/1‑70 (MQ=255)
aCGCAGGCTTTCGTACGCTGTATTCAATCGTGCGTacac > 1:2328480/1‑39 (MQ=38)
cAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTc < 1:887186/47‑1 (MQ=255)
cAGGCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATc < 1:2311412/71‑1 (MQ=255)
ggCTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCAc > 1:3759745/1‑71 (MQ=255)
cTTTCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTcta < 1:1809519/45‑3 (MQ=255)
ttCGTACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCaa > 1:3026173/1‑71 (MQ=255)
tACGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAAt < 1:4658131/46‑1 (MQ=255)
cGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTc > 1:90211/1‑71 (MQ=255)
cGCTGTATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTc > 1:1324630/1‑71 (MQ=255)
cGCTGTATTCCATCGTGCGTAAACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTc < 1:3792271/71‑1 (MQ=255)
|
GATTTAATCGCTGCTGGTGTCGACCCATCAGATATTGTTCTCGATTACGCAGGCTTTCGTACGCTGGATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTC > W3110S.gb/2236524‑2236655
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
