Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,646,699 |
C→G |
Q1415H (CAG→CAC) |
yfhM ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,646,699 | 0 | C | G | 100.0%
| 198.0
/ NA
| 57 | Q1415H (CAG→CAC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (21/36); total (21/36) |
TCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGA > W3110S.gb/2646632‑2646766
|
tCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCctggtg > 1:4041403/1‑70 (MQ=255)
tCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCtggtgc > 1:406403/1‑71 (MQ=255)
cAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGtt > 1:1017868/1‑69 (MQ=255)
aacaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:4173313/1‑70 (MQ=255)
acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:1846910/1‑69 (MQ=255)
acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:585517/1‑69 (MQ=255)
acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:634518/1‑69 (MQ=255)
acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:737827/1‑69 (MQ=255)
acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:935880/1‑69 (MQ=255)
acaacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCa > 1:1006011/1‑69 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCTTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:4300880/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:797382/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:516458/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:460126/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:997773/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:966264/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:4048888/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3860822/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3801524/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3586493/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3229554/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:3212024/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:1492791/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:1715721/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:283688/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:2190806/71‑1 (MQ=255)
acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:2518781/71‑1 (MQ=255)
cgcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtgt < 1:2042211/71‑1 (MQ=255)
cgcgTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:1199811/70‑1 (MQ=255)
ttAGTTTGCTGGGTCGATAGCCAGCGTTCACGAAACGCCTGGTGCGAAAGTGTGTTCAGCATAgtgt < 1:2396061/67‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:736371/48‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:1675269/48‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:938885/48‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:2663567/48‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:944327/48‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtgt < 1:2502495/67‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:4222183/66‑1 (MQ=255)
ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:317511/66‑1 (MQ=255)
tACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtgt < 1:346958/66‑1 (MQ=255)
tACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTAc > 1:978141/1‑68 (MQ=255)
aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGc > 1:1501098/1‑70 (MQ=255)
aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCt > 1:3722500/1‑71 (MQ=255)
aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCt > 1:2800025/1‑71 (MQ=255)
aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCt > 1:2837393/1‑71 (MQ=255)
aCTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCt > 1:2842774/1‑71 (MQ=255)
aGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGtt > 1:3916963/1‑70 (MQ=255)
aGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGtt > 1:1823461/1‑70 (MQ=255)
cAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTAtt < 1:413253/71‑1 (MQ=255)
gTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTttct < 1:4544212/71‑1 (MQ=255)
gTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTttct > 1:3274269/1‑71 (MQ=255)
cACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTg > 1:120724/1‑43 (MQ=255)
cACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTAt > 1:1153252/1‑63 (MQ=255)
cACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTttcttc > 1:2637997/1‑70 (MQ=255)
cACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTttcttc > 1:3163153/1‑70 (MQ=255)
cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc < 1:894986/71‑1 (MQ=255)
cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc < 1:519572/71‑1 (MQ=255)
cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc < 1:3003196/71‑1 (MQ=255)
cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc < 1:28776/71‑1 (MQ=255)
aaaCGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCa < 1:1411636/71‑1 (MQ=255)
ctggtgCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGgaga < 1:4610075/71‑1 (MQ=255)
|
TCGTACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGA > W3110S.gb/2646632‑2646766
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
