Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,330,087 |
(G)5→4 |
coding (1270/1434 nt) |
dacB → |
D‑alanyl‑D‑alanine carboxypeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,330,083 | 0 | G | . | 100.0%
| 148.0
/ NA
| 37 | coding (1266/1434 nt) | dacB | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (16/21); total (16/21) |
TGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATA > W3110S.gb/3330013‑3330148
|
tGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg > 1:3446352/1‑71 (MQ=255)
tGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg > 1:3440781/1‑71 (MQ=255)
aGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg < 1:677167/71‑1 (MQ=255)
aGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg < 1:3456750/71‑1 (MQ=255)
tACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCTggggta < 1:593940/71‑3 (MQ=255)
tACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAggggta < 1:471403/71‑3 (MQ=255)
gTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGtatata < 1:1710023/71‑1 (MQ=255)
cAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAAcc > 1:1753458/1‑71 (MQ=255)
cAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAAcc > 1:4284963/1‑71 (MQ=255)
cAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAAc < 1:2841650/70‑1 (MQ=255)
cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg > 1:810294/1‑71 (MQ=255)
cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg > 1:763440/1‑71 (MQ=255)
cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg > 1:4183071/1‑71 (MQ=255)
cTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCg > 1:2317923/1‑71 (MQ=255)
tGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGtatata < 1:1210607/62‑1 (MQ=255)
cATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg > 1:3569109/1‑54 (MQ=255)
tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg < 1:4427536/49‑1 (MQ=255)
tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAg < 1:3709727/49‑1 (MQ=255)
tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt < 1:167788/71‑1 (MQ=255)
tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt < 1:361829/71‑1 (MQ=255)
tCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt < 1:3889751/71‑1 (MQ=255)
cAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt > 1:3999332/1‑70 (MQ=255)
aGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTc < 1:1523793/70‑1 (MQ=255)
aGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTc < 1:4325535/70‑1 (MQ=255)
aGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCa > 1:3944524/1‑71 (MQ=255)
ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCAt < 1:4597545/71‑1 (MQ=255)
ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCAt < 1:4295362/71‑1 (MQ=255)
ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCAt < 1:393799/71‑1 (MQ=255)
ggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAgggg < 1:3101290/45‑1 (MQ=255)
cgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca > 1:2242588/1‑70 (MQ=255)
gatggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGAtt > 1:3339142/1‑58 (MQ=255)
gatggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACagcg < 1:1014817/71‑1 (MQ=255)
atggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACagcga < 1:1603713/71‑1 (MQ=255)
ggaAAAGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACagcgagc > 1:4173600/1‑71 (MQ=255)
agaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca > 1:463377/3‑60 (MQ=255)
aaaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca > 1:1045392/1‑60 (MQ=255)
aaaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca > 1:1464314/1‑60 (MQ=255)
aaaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACca > 1:2376626/1‑60 (MQ=255)
aaGTCTCAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGc < 1:2259506/71‑1 (MQ=255)
tcAGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTc < 1:2538892/47‑1 (MQ=39)
aGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACg < 1:2258143/68‑1 (MQ=255)
aGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACg < 1:3838303/68‑1 (MQ=255)
aGCGAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACg < 1:408901/68‑1 (MQ=255)
gAAAACCGGTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGc > 1:4209147/1‑71 (MQ=255)
gTTCGTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGc < 1:3821060/71‑1 (MQ=255)
gTTGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAata < 1:3459061/71‑1 (MQ=255)
ttGCA‑GGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCaa < 1:1862508/68‑1 (MQ=255)
|
TGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATA > W3110S.gb/3330013‑3330148
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
