Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,703,672 G→A A355T (GCC→ACC)  aroB → 3‑dehydroquinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,703,6720GA100.0% 379.7 / NA 114A355T (GCC→ACC) aroB3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/62);  total (52/62)

Only 100 of 114 total aligned reads displayed.

AATTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGC  >  minE/2703605‑2703739
                                                                   |                                                                   
aaTTCTTCCGTTGGCAATTGGTAAGAGTTAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:3121925/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACAcc                                                                   >  1:3203258/1‑70 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACAcc                                                                   >  1:1220657/1‑70 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACAcc                                                                   >  1:2462190/1‑70 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:2972452/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:2824161/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:190891/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:1841884/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:179769/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:3407567/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  >  1:257902/1‑71 (MQ=255)
aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGAACGAGCTTGTTCTTAACACCa                                                                  >  1:1263252/1‑71 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:1891919/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:4211875/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:2591590/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:2344237/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:2036493/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:3529633/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:4285613/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:1462825/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:279697/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:2812089/70‑1 (MQ=255)
        cGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:3119375/70‑1 (MQ=255)
              cAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCTTTGCCGATTGTCaa                                                    <  1:1338332/71‑1 (MQ=255)
              cAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCaa                                                    <  1:2802087/71‑1 (MQ=255)
              cAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCaa                                                    <  1:2288508/71‑1 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCaa                                                    >  1:3337700/1‑70 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:3442264/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:3226252/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:2301537/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:3617893/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:4149798/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:939374/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:773513/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:1337313/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:558154/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:198371/1‑71 (MQ=255)
               aaTTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATGGCCGATTGTCAAt                                                   >  1:3958932/1‑71 (MQ=255)
                         agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaac                                          >  1:2321902/1‑70 (MQ=255)
                         agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaac                                          >  1:3449907/1‑70 (MQ=255)
                         agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaac                                          >  1:4119350/1‑70 (MQ=255)
                         agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaac                                          >  1:3821576/1‑70 (MQ=255)
                         agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaa                                           >  1:2351866/1‑69 (MQ=255)
                          gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa                                        >  1:1749686/1‑71 (MQ=255)
                          gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa                                        >  1:2472967/1‑71 (MQ=255)
                          gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa                                        >  1:1093380/1‑71 (MQ=255)
                          gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa                                        >  1:1484970/1‑71 (MQ=255)
                          gTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaa                                        >  1:1172932/1‑71 (MQ=255)
                           tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaac                                       <  1:3464230/71‑1 (MQ=255)
                                  cgaaGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:4350686/67‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACAGCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:2138902/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:2150860/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:653554/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:3702479/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:470672/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:4184702/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:108571/70‑1 (MQ=255)
                                  cGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:3095542/70‑1 (MQ=255)
                                    cAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:684880/68‑1 (MQ=255)
                                    cAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaaa                                 <  1:2784276/68‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCat                           <  1:1423311/71‑2 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:2936494/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:1815255/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:3257193/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:680248/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:1272766/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:1613966/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:2371370/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:3576118/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:1982519/71‑1 (MQ=255)
                                       cggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:1482632/71‑1 (MQ=255)
                                        ggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:1443137/70‑1 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCGGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:232031/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:1621584/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:295828/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:154069/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:1500640/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:454406/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:615347/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:1257698/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:1040264/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:3109034/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:1032428/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:3457998/1‑71 (MQ=255)
                                             ttCGCACGAGCTTGTTCCTGACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:520253/1‑71 (MQ=255)
                                             atCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:2758494/2‑71 (MQ=255)
                                                     aGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAg                           <  1:2092649/57‑1 (MQ=255)
                                                       cTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCgg           <  1:3863789/71‑1 (MQ=255)
                                                       cTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCgg           <  1:1912624/71‑1 (MQ=255)
                                                       cTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCgg           <  1:4003563/71‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:3614383/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:3623657/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:551941/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:1975485/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:2729071/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:2749807/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:1534969/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCt        <  1:1174377/70‑1 (MQ=255)
                                                           ttCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGGCAGGCCGCTTATCAAGCGGTCt        <  1:799470/70‑1 (MQ=255)
                                                                aaCACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGc  >  1:525709/1‑71 (MQ=255)
                                                                   |                                                                   
AATTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGC  >  minE/2703605‑2703739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A