breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 52,517 | T→C | E757E (GAA→GAG) | imp ← | exported protein required for envelope biosynthesis and integrity |
RA | 91,462 | T→C | S56P (TCT→CCT) | lpxC → | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
RA | 144,272 | C→A | G194V (GGC→GTC) | yadB ← | glutamyl‑Q tRNA(Asp) synthetase |
RA | 195,616 | C→T | L71L (CTG→CTA) | yaeF ← | predicted lipoprotein |
RA | 204,556 | A→G | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
RA | 575,416 | T→C | N135S (AAT→AGT) | ybjX ← | conserved hypothetical protein |
RA | 668,428 | C→A | T589K (ACG→AAG) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
RA | 692,900 | T→C | A144A (GCT→GCC) | hyaD → | protein involved in processing of HyaA and HyaB proteins |
RA | 782,109 | T→C | Q167R (CAG→CGG) | hlyE ← | hemolysin E |
RA | 798,949 | C→T | M214I (ATG→ATA) | treA ← | periplasmic trehalase |
RA | 899,984 | (T)9→8 | intergenic (+41/‑172) | pspE → / → ycjM | thiosulfate:cyanide sulfurtransferase/predicted glucosyltransferase |
RA | 1,000,186 | G→A | G179D (GGT→GAT) | ydgD → | predicted peptidase |
RA | 1,013,547 | G→A | R245C (CGC→TGC) | fumC ← | fumarate hydratase (fumarase C), aerobic Class II |
RA | 1,036,505 | G→A | A632A (GCG→GCA) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
RA | 1,081,858 | T→C | N104S (AAC→AGC) | ydhY ← | predicted 4Fe‑4S ferridoxin‑type protein |
RA | 1,097,909 | T→C | intergenic (+32/‑397) | ydiK → / → ydiL | predicted inner membrane protein/conserved hypothetical protein |
RA | 1,099,149 | A→G | T130A (ACA→GCA) | ydiM → | predicted transporter |
RA | 1,151,211 | A→G | V2V (GTA→GTG) | ydjQ → | endonuclease of nucleotide excision repair |
RA | 1,231,014 | C→T | G581D (GGT→GAT) | ptrB ← | protease II |
RA | 1,252,050 | T→C | K6K (AAA→AAG) | yebC ← | conserved hypothetical protein |
RA | 1,253,716 | C→T | A241A (GCG→GCA) | aspS ← | aspartyl‑tRNA synthetase |
RA | 1,269,468 | (A)7→8 | intergenic (‑18/‑282) | uvrC ← / → yedP | excinuclease UvrABC, endonuclease subunit/conserved hypothetical protein |
RA | 1,302,228 | C→T | V285M (GTG→ATG) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase |
RA | 1,325,522 | T→G | K71T (AAA→ACA) | wcaL ← | predicted glycosyl transferase |
RA | 1,330,586 | A→G | Y334H (TAC→CAC) | cpsG ← | phosphomannomutase |
RA | 1,352,427 | T→C | N101D (AAC→GAC) | thiD ← | bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
RA | 1,439,412 | G→A | A297V (GCT→GTT) | ompC ← | outer membrane porin protein C |
RA | 1,509,579 | G→A | A42T (GCA→ACA) | ackA → | acetate kinase A and propionate kinase 2 |
RA | 1,511,849 | T→C | W373R (TGG→CGG) | pta → | phosphate acetyltransferase |
RA | 1,523,658 | C→T | Q153* (CAA→TAA) | flk → | predicted flagella assembly protein |
RA | 1,536,834 | C→T | M1I (ATG→ATA) † | yfcR ← | predicted fimbrial‑like adhesin protein |
RA | 1,591,415 | G→T | K203N (AAG→AAT) | yfeS → | conserved hypothetical protein |
RA | 1,643,860 | A→G | R9R (CGT→CGC) | purC ← | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
RA | 1,647,049 | C→T | Q102* (CAA→TAA) | bcp → | thiol peroxidase, thioredoxin‑dependent |
RA | 1,664,529 | G→A | I231I (ATC→ATT) | guaA ← | GMP synthetase |
RA | 1,680,754 | T→C | Y1407C (TAC→TGC) | yfhM ← | conserved hypothetical protein |
RA | 1,695,295 | G→A | Q239* (CAG→TAG) | yfhQ ← | predicted methyltransferase |
RA | 1,700,462 | A→G | L24P (CTA→CCA) | hcaT ← | predicted 3‑phenylpropionic transporter |
RA | 1,777,713 | T→C | N105D (AAT→GAT) | trmD ← | tRNA (guanine‑1‑)‑methyltransferase |
RA | 1,784,184 | T→C | I223T (ATA→ACA) | yfjB → | NAD kinase |
RA | 1,787,235 | G→A | S72S (AGC→AGT) | yfjG ← | conserved hypothetical protein |
RA | 1,827,842 | Δ1 bp | coding (1085/2562 nt) | mutS → | methyl‑directed mismatch repair protein |
RA | 1,833,531 | C→T | R92C (CGC→TGC) | ygbL → | predicted class II aldolase |
RA | 1,867,615 | G→A | T429I (ACC→ATC) | ygcU ← | predicted FAD containing dehydrogenase |
RA | 1,892,019 | +GC | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
RA | 2,187,308 | T→C | P183P (CCA→CCG) | nanT ← | sialic acid transporter |
RA | 2,279,749 | Δ1 bp | coding (633/1101 nt) | trmA → | tRNA (uracil‑5‑)‑methyltransferase |
RA | 2,364,103 | A→G | W456R (TGG→CGG) | trkH ← | potassium transporter |
RA | 2,371,447 | A→G | F214L (TTT→CTT) | fre ← | flavin reductase |
RA | 2,450,298 | A→G | W78R (TGG→CGG) | yifE ← | conserved hypothetical protein |
RA | 2,473,718 | C→T | F331F (TTC→TTT) | glmS → | L‑glutamine:D‑fructose‑6‑phosphate aminotransferase |
RA | 2,512,887 | G→A | V27I (GTC→ATC) | gltS → | glutamate transporter |
RA | 2,516,656 | C→T | A102T (GCT→ACT) | trmH ← | tRNA (Guanosine‑2'‑O‑)‑methyltransferase |
RA | 2,605,272 | A→G | L50L (CTT→CTC) | uspA ← | universal stress global response regulator |
RA | 2,632,970 | A→G | K421R (AAA→AGA) | livM → | leucine/isoleucine/valine transporter subunit |
RA | 2,639,808 | A→G | T245A (ACG→GCG) *147Q (TAA→CAA) |
ugpQ → yhhA ← |
glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
RA | 2,681,918 | A→G | R598R (CGT→CGC) | yhgF ← | predicted transcriptional accessory protein |
RA | 2,703,672 | G→A | A355T (GCC→ACC) | aroB → | 3‑dehydroquinate synthase |
RA | 2,726,174 | G→A | R303R (CGC→CGT) | yheS ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
RA | 2,802,519 | T→A | D196V (GAT→GTT) | qor ← | quinone oxidoreductase, NADPH‑dependent |
RA | 2,952,505 | (G)8→7 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | minE | 92528 | 92536 | 9 | 7 [3] | [0] 14 | secM | regulator of secA translation |
* | * | ÷ | minE | 127063 | 127080 | 18 | 6 [0] | [3] 4 | can | carbonic anhydrase |
* | * | ÷ | minE | 149315 | 149364 | 50 | 8 [1] | [3] 9 | hrpB/mrcB | predicted ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
* | * | ÷ | minE | 251997 | 252016 | 20 | 9 [0] | [0] 13 | malZ | maltodextrin glucosidase |
* | * | ÷ | minE | 444342 | 444404 | 63 | 9 [1] | [0] 12 | [kdpE] | [kdpE] |
* | * | ÷ | minE | 750874 | 750903 | 30 | 4 [0] | [2] 5 | mfd | transcription‑repair coupling factor |
* | * | ÷ | minE | 815596 | 815607 | 12 | 8 [0] | [3] 5 | lolB | chaperone for lipoproteins |
* | * | ÷ | minE | 865539 | 865586 | 48 | 7 [0] | [2] 8 | yciQ | predicted inner membrane protein |
* | * | ÷ | minE | 875454 | 875473 | 20 | 5 [0] | [1] 16 | acnA | aconitate hydratase 1 |
* | * | ÷ | minE | 899410 | 899437 | 28 | 14 [0] | [0] 4 | pspD | peripheral inner membrane phage‑shock protein |
* | * | ÷ | minE | 1009234 | 1009234 | 1 | 5 [0] | [0] 11 | folM | dihydrofolate reductase isozyme |
* | * | ÷ | minE | 1154078 | 1154117 | 40 | 7 [0] | [0] 10 | astE | succinylglutamate desuccinylase |
* | * | ÷ | minE | 1228579 | 1228582 | 4 | 19 [0] | [0] 13 | yobA | conserved hypothetical protein |
* | * | ÷ | minE | 1231162 | 1231174 | 13 | 5 [0] | [2] 5 | ptrB | protease II |
* | * | ÷ | minE | 1381747 | 1381753 | 7 | 5 [1] | [0] 14 | yeiB | conserved inner membrane protein |
* | * | ÷ | minE | 1420598 | 1420615 | 18 | 5 [0] | [0] 5 | ccmF | heme lyase, CcmF subunit |
* | * | ÷ | minE | 1494514 | 1494523 | 10 | 6 [0] | [0] 16 | nuoG | NADH:ubiquinone oxidoreductase, chain G |
* | * | ÷ | minE | 1499365 | 1499372 | 8 | 6 [0] | [0] 9 | nuoC | NADH:ubiquinone oxidoreductase, chain C,D |
* | * | ÷ | minE | 1568411 | 1568414 | 4 | 4 [0] | [0] 9 | mntH | manganese/divalent cation transporter |
* | * | ÷ | minE | 1616684 | 1616750 | 67 | 7 [0] | [0] 9 | eutE | predicted aldehyde dehydrogenase, ethanolamine utilization protein |
* | * | ÷ | minE | 1616822 | 1616836 | 15 | 9 [0] | [0] 21 | eutE | predicted aldehyde dehydrogenase, ethanolamine utilization protein |
* | * | ÷ | minE | 1618982 | 1619033 | 52 | 17 [0] | [0] 7 | eutI | predicted phosphotransacetylase subunit |
* | * | ÷ | minE | 1752091 | 1752103 | 13 | 9 [1] | [0] 4 | yfiP | conserved hypothetical protein |
* | * | ÷ | minE | 1945323 | 1945360 | 38 | 7 [2] | [0] 9 | ygfF/gcvP | predicted NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex |
* | * | ÷ | minE | 2122852 | 2122856 | 5 | 12 [0] | [0] 19 | yraQ | predicted permease |
* | * | ÷ | minE | 2640555 | 2640568 | 14 | 4 [0] | [0] 6 | ggt | gamma‑glutamyltranspeptidase |
* | * | ÷ | minE | 2858102 | 2858161 | 60 | 18 [0] | [1] 4 | amiB | N‑acetylmuramoyl‑l‑alanine amidase II |
* | * | ÷ | minE | 2919180 | 2919183 | 4 | 5 [0] | [0] 7 | argI | ornithine carbamoyltransferase 1 |