breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 52,517 T→C E757E (GAA→GAG imp ← exported protein required for envelope biosynthesis and integrity
RA 91,462 T→C S56P (TCT→CCT)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
RA 144,272 C→A G194V (GGC→GTC)  yadB ← glutamyl‑Q tRNA(Asp) synthetase
RA 195,616 C→T L71L (CTG→CTA yaeF ← predicted lipoprotein
RA 204,556 A→G intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 575,416 T→C N135S (AAT→AGT)  ybjX ← conserved hypothetical protein
RA 668,428 C→A T589K (ACG→AAG)  uup → fused predicted transporter subunits and ATP‑binding components of ABC superfamily
RA 692,900 T→C A144A (GCT→GCC hyaD → protein involved in processing of HyaA and HyaB proteins
RA 782,109 T→C Q167R (CAG→CGG)  hlyE ← hemolysin E
RA 798,949 C→T M214I (ATG→ATA treA ← periplasmic trehalase
RA 899,984 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase/predicted glucosyltransferase
RA 1,000,186 G→A G179D (GGT→GAT)  ydgD → predicted peptidase
RA 1,013,547 G→A R245C (CGC→TGC)  fumC ← fumarate hydratase (fumarase C), aerobic Class II
RA 1,036,505 G→A A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,081,858 T→C N104S (AAC→AGC)  ydhY ← predicted 4Fe‑4S ferridoxin‑type protein
RA 1,097,909 T→C intergenic (+32/‑397) ydiK → / → ydiL predicted inner membrane protein/conserved hypothetical protein
RA 1,099,149 A→G T130A (ACA→GCA)  ydiM → predicted transporter
RA 1,151,211 A→G V2V (GTA→GTG ydjQ → endonuclease of nucleotide excision repair
RA 1,231,014 C→T G581D (GGT→GAT)  ptrB ← protease II
RA 1,252,050 T→C K6K (AAA→AAG yebC ← conserved hypothetical protein
RA 1,253,716 C→T A241A (GCG→GCA aspS ← aspartyl‑tRNA synthetase
RA 1,269,468 (A)7→8 intergenic (‑18/‑282) uvrC ← / → yedP excinuclease UvrABC, endonuclease subunit/conserved hypothetical protein
RA 1,302,228 C→T V285M (GTG→ATG)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase
RA 1,325,522 T→G K71T (AAA→ACA)  wcaL ← predicted glycosyl transferase
RA 1,330,586 A→G Y334H (TAC→CAC)  cpsG ← phosphomannomutase
RA 1,352,427 T→C N101D (AAC→GAC)  thiD ← bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase
RA 1,439,412 G→A A297V (GCT→GTT)  ompC ← outer membrane porin protein C
RA 1,509,579 G→A A42T (GCA→ACA)  ackA → acetate kinase A and propionate kinase 2
RA 1,511,849 T→C W373R (TGG→CGG)  pta → phosphate acetyltransferase
RA 1,523,658 C→T Q153* (CAA→TAA)  flk → predicted flagella assembly protein
RA 1,536,834 C→T M1I (ATG→ATA) † yfcR ← predicted fimbrial‑like adhesin protein
RA 1,591,415 G→T K203N (AAG→AAT yfeS → conserved hypothetical protein
RA 1,643,860 A→G R9R (CGT→CGC purC ← phosphoribosylaminoimidazole‑succinocarboxamide synthetase
RA 1,647,049 C→T Q102* (CAA→TAA)  bcp → thiol peroxidase, thioredoxin‑dependent
RA 1,664,529 G→A I231I (ATC→ATT guaA ← GMP synthetase
RA 1,680,754 T→C Y1407C (TAC→TGC)  yfhM ← conserved hypothetical protein
RA 1,695,295 G→A Q239* (CAG→TAG)  yfhQ ← predicted methyltransferase
RA 1,700,462 A→G L24P (CTA→CCA)  hcaT ← predicted 3‑phenylpropionic transporter
RA 1,777,713 T→C N105D (AAT→GAT)  trmD ← tRNA (guanine‑1‑)‑methyltransferase
RA 1,784,184 T→C I223T (ATA→ACA)  yfjB → NAD kinase
RA 1,787,235 G→A S72S (AGC→AGT yfjG ← conserved hypothetical protein
RA 1,827,842 Δ1 bp coding (1085/2562 nt) mutS → methyl‑directed mismatch repair protein
RA 1,833,531 C→T R92C (CGC→TGC)  ygbL → predicted class II aldolase
RA 1,867,615 G→A T429I (ACC→ATC)  ygcU ← predicted FAD containing dehydrogenase
RA 1,892,019 +GC intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 2,187,308 T→C P183P (CCA→CCG nanT ← sialic acid transporter
RA 2,279,749 Δ1 bp coding (633/1101 nt) trmA → tRNA (uracil‑5‑)‑methyltransferase
RA 2,364,103 A→G W456R (TGG→CGG)  trkH ← potassium transporter
RA 2,371,447 A→G F214L (TTT→CTT)  fre ← flavin reductase
RA 2,450,298 A→G W78R (TGG→CGG)  yifE ← conserved hypothetical protein
RA 2,473,718 C→T F331F (TTC→TTT glmS → L‑glutamine:D‑fructose‑6‑phosphate aminotransferase
RA 2,512,887 G→A V27I (GTC→ATC)  gltS → glutamate transporter
RA 2,516,656 C→T A102T (GCT→ACT)  trmH ← tRNA (Guanosine‑2'‑O‑)‑methyltransferase
RA 2,605,272 A→G L50L (CTT→CTC uspA ← universal stress global response regulator
RA 2,632,970 A→G K421R (AAA→AGA)  livM → leucine/isoleucine/valine transporter subunit
RA 2,639,808 A→G T245A (ACG→GCG) 
*147Q (TAA→CAA) 
ugpQ →
yhhA ←
glycerophosphodiester phosphodiesterase, cytosolic
conserved hypothetical protein
RA 2,681,918 A→G R598R (CGT→CGC yhgF ← predicted transcriptional accessory protein
RA 2,703,672 G→A A355T (GCC→ACC)  aroB → 3‑dehydroquinate synthase
RA 2,726,174 G→A R303R (CGC→CGT yheS ← fused predicted transporter subunits and ATP‑binding components of ABC superfamily
RA 2,802,519 T→A D196V (GAT→GTT)  qor ← quinone oxidoreductase, NADPH‑dependent
RA 2,952,505 (G)8→7 noncoding (72/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ minE 92528 92536 9 7 [3] [0] 14 secM regulator of secA translation
* * ÷ minE 127063 127080 18 6 [0] [3] 4 can carbonic anhydrase
* * ÷ minE 149315 149364 50 8 [1] [3] 9 hrpB/mrcB predicted ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
* * ÷ minE 251997 252016 20 9 [0] [0] 13 malZ maltodextrin glucosidase
* * ÷ minE 444342 444404 63 9 [1] [0] 12 [kdpE] [kdpE]
* * ÷ minE 750874 750903 30 4 [0] [2] 5 mfd transcription‑repair coupling factor
* * ÷ minE 815596 815607 12 8 [0] [3] 5 lolB chaperone for lipoproteins
* * ÷ minE 865539 865586 48 7 [0] [2] 8 yciQ predicted inner membrane protein
* * ÷ minE 875454 875473 20 5 [0] [1] 16 acnA aconitate hydratase 1
* * ÷ minE 899410 899437 28 14 [0] [0] 4 pspD peripheral inner membrane phage‑shock protein
* * ÷ minE 1009234 1009234 1 5 [0] [0] 11 folM dihydrofolate reductase isozyme
* * ÷ minE 1154078 1154117 40 7 [0] [0] 10 astE succinylglutamate desuccinylase
* * ÷ minE 1228579 1228582 4 19 [0] [0] 13 yobA conserved hypothetical protein
* * ÷ minE 1231162 1231174 13 5 [0] [2] 5 ptrB protease II
* * ÷ minE 1381747 1381753 7 5 [1] [0] 14 yeiB conserved inner membrane protein
* * ÷ minE 1420598 1420615 18 5 [0] [0] 5 ccmF heme lyase, CcmF subunit
* * ÷ minE 1494514 1494523 10 6 [0] [0] 16 nuoG NADH:ubiquinone oxidoreductase, chain G
* * ÷ minE 1499365 1499372 8 6 [0] [0] 9 nuoC NADH:ubiquinone oxidoreductase, chain C,D
* * ÷ minE 1568411 1568414 4 4 [0] [0] 9 mntH manganese/divalent cation transporter
* * ÷ minE 1616684 1616750 67 7 [0] [0] 9 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ minE 1616822 1616836 15 9 [0] [0] 21 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ minE 1618982 1619033 52 17 [0] [0] 7 eutI predicted phosphotransacetylase subunit
* * ÷ minE 1752091 1752103 13 9 [1] [0] 4 yfiP conserved hypothetical protein
* * ÷ minE 1945323 1945360 38 7 [2] [0] 9 ygfF/gcvP predicted NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
* * ÷ minE 2122852 2122856 5 12 [0] [0] 19 yraQ predicted permease
* * ÷ minE 2640555 2640568 14 4 [0] [0] 6 ggt gamma‑glutamyltranspeptidase
* * ÷ minE 2858102 2858161 60 18 [0] [1] 4 amiB N‑acetylmuramoyl‑l‑alanine amidase II
* * ÷ minE 2919180 2919183 4 5 [0] [0] 7 argI ornithine carbamoyltransferase 1