Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I2 R1 330 0.0 2544031 91.9% 2337964 62.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,068,568 T→A G22G (GGT→GGA)  ymdF → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,068,5680TA100.0% 186.6 / NA 56G22G (GGT→GGA) ymdFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/22);  total (34/22)

CAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGA  >  W3110S.gb/1068537‑1068627
                               |                                                           
cAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAAttt                             <  1:1013323/64‑1 (MQ=255)
 agaagaCCGCGAAAGCGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:2016920/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:17741/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:585503/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:590136/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:583417/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:2111924/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:616912/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:664310/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:1934394/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:498426/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:2255823/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:1806152/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:2303231/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:776839/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:2426427/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:82444/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:2455281/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:276256/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:473523/64‑1 (MQ=255)
 agaagaCCGCGAAAGAGCATCAGAAACAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:1651586/64‑1 (MQ=255)
  gaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTc                            <  1:1303377/63‑1 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGTGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:455609/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTg   >  1:1871543/1‑64 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:601575/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:671344/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:487235/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:773314/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:789562/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:812811/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:480468/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:845245/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:857849/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:975619/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2306058/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2018920/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1077368/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1103268/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1174516/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1188058/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1306683/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1318497/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1391201/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1504575/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1923742/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:1973201/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:421350/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:212736/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2156586/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2219659/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2234835/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2241704/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2278219/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:2450666/1‑65 (MQ=255)
                          gCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:34013/1‑65 (MQ=255)
                          gCAGGAAAAAAAGCTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa  >  1:285913/1‑65 (MQ=255)
                               |                                                           
CAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGA  >  W3110S.gb/1068537‑1068627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A