Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,068,568 |
T→A |
G22G (GGT→GGA) |
ymdF → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,068,568 | 0 | T | A | 100.0%
| 105.1
/ NA
| 36 | G22G (GGT→GGA) | ymdF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (23/13); total (23/13) |
AATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAG > W3110S.gb/1068502‑1068629
|
aaTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaa > 1:676362/1‑70 (MQ=255)
aTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGaaaaaa < 1:3361608/71‑1 (MQ=255)
aTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaa > 1:1114940/1‑70 (MQ=255)
tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa < 1:1910056/71‑1 (MQ=255)
tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa < 1:1100890/71‑1 (MQ=255)
gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:944184/1‑71 (MQ=255)
gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:4571207/1‑71 (MQ=255)
gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:1832254/1‑71 (MQ=255)
gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:1946649/1‑71 (MQ=255)
aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTg < 1:2813771/70‑1 (MQ=255)
aaCCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTg < 1:2804352/70‑1 (MQ=255)
ccATCGAGGCGGTTCCGGCAATTTTGCAGAAGACAGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGa > 1:2637177/1‑70 (MQ=255)
gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:3174507/1‑61 (MQ=255)
gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:4487901/1‑61 (MQ=255)
gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGacagca > 1:4480529/1‑70 (MQ=255)
gAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGa > 1:705419/1‑65 (MQ=255)
tCCGGCAATTTTGCAGAAGCCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggg > 1:2201901/1‑71 (MQ=39)
tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggg > 1:1754822/1‑71 (MQ=255)
tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggg > 1:3838124/1‑71 (MQ=255)
accGGCAATTTTGCAGAAGCCCGCGAAAGAGCATCAGAAGCAGGAAAAAAATGTGGACAGCACAGCggggg > 1:3160096/2‑71 (MQ=255)
ccGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggt > 1:4450420/1‑69 (MQ=255)
ccGGCAATTTTGCAGAAGACAGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCggggt > 1:1459272/1‑69 (MQ=255)
cGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTa < 1:2026584/71‑1 (MQ=255)
cGGCAATTTTGAAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTa < 1:4350992/71‑1 (MQ=255)
tttGGAGAAGACCGAGAAAGAGCATCAGAAGCAGGAAAAAAAGGTg < 1:3953824/46‑1 (MQ=37)
tGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg < 1:4112040/70‑1 (MQ=255)
gCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGa < 1:4682968/70‑1 (MQ=255)
agaagaCCGCGAAAGAGCATCAGAATCAGGAAAAAAAGGTGGACAGCACAGCGGTGGTaa < 1:944984/60‑1 (MQ=38)
agaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTaa < 1:825640/60‑1 (MQ=255)
gaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGa < 1:684940/71‑1 (MQ=255)
ccGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAAttt > 1:1942290/1‑57 (MQ=255)
ccGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAAttt > 1:1346200/1‑57 (MQ=255)
agagCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGAcc > 1:3917567/1‑61 (MQ=255)
agagCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGAcc > 1:3101335/1‑61 (MQ=255)
gagCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCa > 1:1257390/1‑71 (MQ=255)
agCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCAt > 1:3357109/1‑71 (MQ=255)
gCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgcgcgc < 1:942065/68‑1 (MQ=255)
aGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAg > 1:701938/1‑71 (MQ=255)
|
AATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAG > W3110S.gb/1068502‑1068629
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
