Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,346,506 |
C→T |
E651K (GAA→AAA) |
gmr ← |
modulator of Rnase II stability |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,346,506 | 0 | C | T | 76.0%
| 41.7
/ 12.6
| 25 | E651K (GAA→AAA) | gmr | modulator of Rnase II stability |
| Reads supporting (aligned to +/- strand): ref base C (6/0); new base T (19/0); total (25/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 |
TAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTG > W3110S.gb/1346450‑1346531
|
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1210150/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:842566/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:814891/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:685535/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:623870/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:603543/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:440654/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:391244/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:249946/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:2279914/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:2031857/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1943244/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1838519/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1742036/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:164364/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1546166/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1442646/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcgg > 1:1003502/1‑61 (MQ=255)
tAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTTGAAgcg > 1:2479331/1‑61 (MQ=255)
tACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg > 1:1937749/1‑64 (MQ=255)
tACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg > 1:2336855/1‑64 (MQ=255)
tACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg > 1:1746329/1‑64 (MQ=255)
tACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg > 1:1462556/1‑64 (MQ=255)
tACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg > 1:702827/1‑64 (MQ=255)
tACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTg > 1:913711/1‑64 (MQ=255)
|
TAAGTATATATATTCATCTACTTATGCGCGCTTCAGATAGCGTTTATACCAGCGTTCGAAGGCGACGGCGGGCATCGGTTTG > W3110S.gb/1346450‑1346531
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
