Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,346,872 |
C→T |
41.9% |
V529I (GTT→ATT) |
gmr ← |
modulator of Rnase II stability |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,346,872 | 0 | C | T | 41.9%
| 24.0
/ 49.4
| 43 | V529I (GTT→ATT) | gmr | modulator of Rnase II stability |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base T (9/9); total (21/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGA > W3110S.gb/1346805‑1346939
|
cTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc < 1:2747878/71‑1 (MQ=255)
cTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc < 1:473216/71‑1 (MQ=255)
aaaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta > 1:2323666/1‑71 (MQ=255)
aaaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta > 1:1602908/1‑71 (MQ=255)
aaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCta < 1:736329/70‑1 (MQ=255)
aaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta < 1:188203/70‑1 (MQ=255)
tgAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACCCTCTGTCAGTTCAACATCTATAggg > 1:1254889/1‑69 (MQ=255)
tAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta < 1:1936660/60‑1 (MQ=255)
gTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTg < 1:1866675/71‑1 (MQ=255)
gTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTg < 1:2325698/71‑1 (MQ=255)
ttCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc < 1:2122510/42‑1 (MQ=255)
ttCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta < 1:1831083/44‑1 (MQ=255)
ttCATCATTCTCAATCAGACAAATCTCTGTCAGTTCAACATCTATAGGGCAg < 1:2780451/52‑1 (MQ=255)
tcatcaTTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCaa > 1:1823902/1‑58 (MQ=255)
catcatTCTCAATCAGACAACTCTCTGTCAGTTCTATATCTATAGGGCAGTATTCAAAATTGAGTTCCTg < 1:2028606/70‑1 (MQ=255)
catcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCta > 1:1862820/1‑42 (MQ=255)
catcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTg < 1:2565786/70‑1 (MQ=255)
catcatTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTg < 1:62618/70‑1 (MQ=255)
cataatTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTg > 1:593112/1‑70 (MQ=255)
atcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCta < 1:1266720/41‑1 (MQ=255)
tcatTCTCAATCAGACAACTCTCTGTCAGTTCAAc < 1:212382/35‑1 (MQ=255)
tcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTg > 1:1194915/1‑68 (MQ=255)
tcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGgag < 1:437015/71‑1 (MQ=255)
catTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAg < 1:1471953/47‑1 (MQ=255)
ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:1246008/1‑46 (MQ=255)
ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:348186/1‑46 (MQ=255)
ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:1429270/1‑46 (MQ=255)
ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:1620567/1‑46 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:894875/49‑1 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTg < 1:1321067/50‑1 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTg < 1:295602/50‑1 (MQ=255)
aGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAAt > 1:803919/1‑46 (MQ=255)
tctctGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGt > 1:2158990/1‑70 (MQ=255)
tctGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGa > 1:2010497/1‑70 (MQ=255)
tctGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGa > 1:504412/1‑70 (MQ=255)
tctGTCAGTTCAACATCTATAGGGCAGTATTCAAAAt > 1:898473/1‑37 (MQ=255)
tctGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCt > 1:1351894/1‑46 (MQ=255)
aGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCa > 1:1575112/1‑55 (MQ=255)
aGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAg > 1:213063/1‑56 (MQ=255)
ttCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGaa < 1:1355963/63‑1 (MQ=255)
cAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGa > 1:1598317/1‑71 (MQ=255)
cAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGga > 1:225577/1‑39 (MQ=255)
cAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTg < 1:240303/70‑1 (MQ=255)
aaCATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTg < 1:506820/69‑1 (MQ=255)
|
CTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGA > W3110S.gb/1346805‑1346939
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
