Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,346,872 |
C→T |
V529I (GTT→ATT) |
gmr ← |
modulator of Rnase II stability |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,346,872 | 0 | C | T | 100.0%
| 133.5
/ NA
| 40 | V529I (GTT→ATT) | gmr | modulator of Rnase II stability |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/11); total (29/11) |
GAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACC > W3110S.gb/1346815‑1346912
|
gAATAACAGACAGTGCCAGTTCATCATTCTCAATGAGACAACTCTCTGTCAGTTCAATATCtata > 1:884400/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:1799935/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:2693239/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:2750623/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:240072/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:209067/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:3110012/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:1747566/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:1736431/1‑65 (MQ=255)
gAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCtata > 1:1171056/1‑65 (MQ=255)
aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:579246/65‑1 (MQ=255)
aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:1923057/65‑1 (MQ=255)
aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:2430839/65‑1 (MQ=255)
aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:2297253/65‑1 (MQ=255)
aGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:881379/65‑1 (MQ=255)
gTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:1821726/64‑1 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCa > 1:358801/1‑45 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCa > 1:3035417/1‑45 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:1014291/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:2976685/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:2985496/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:59204/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:3096102/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:889395/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:310047/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:2667067/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:2455724/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:2358207/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:2223945/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:1841581/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:1786811/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:1459819/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:1286004/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:1253581/1‑46 (MQ=255)
atTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAg > 1:114929/1‑46 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc < 1:2544865/65‑1 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc < 1:2503317/65‑1 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc < 1:1780797/65‑1 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc < 1:1299118/65‑1 (MQ=255)
tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAcc < 1:128941/65‑1 (MQ=255)
|
GAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACC > W3110S.gb/1346815‑1346912
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
