Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,314,973 |
G→T |
F299L (TTC→TTA) |
infB ← |
fused protein chain initiation factor 2, IF2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,314,973 | 0 | G | T | 100.0%
| 11.8
/ NA
| 5 | F299L (TTC→TTA) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (5/0); total (5/0) |
CGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAA > W3110S.gb/3314930‑3314994
|
cGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGTAAGCCTTGCTGCAGCgaagaa > 1:2158205/1‑65 (MQ=255)
cGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGTAAGCCTTGCTGCAGCgaagaa > 1:448515/1‑65 (MQ=255)
cGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGTAAGCCTTGCTGCAGCgaagaa > 1:704084/1‑65 (MQ=255)
cGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGTAAGCCTTGCTGCAGCgaagaa > 1:78320/1‑65 (MQ=255)
cGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGTAAGCCTTGCTGCAGCgaagaa > 1:804420/1‑65 (MQ=255)
|
CGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAA > W3110S.gb/3314930‑3314994
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
