Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I2 R1 330 0.0 2544031 91.9% 2337964 62.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,345,965 +T coding (1394/1434 nt) rpoN → RNA polymerase, sigma 54 (sigma N) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,345,9621.T100.0% 78.5 / NA 23S464F (TCT→TTT) rpoNRNA polymerase, sigma 54 (sigma N) factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (23/0);  total (23/0)

TGGCACGCCGCACTGTTGCGAAGTACCGAGAGTC‑TTTATCCATTCCGCCGTCAAACCAGCGTA  >  W3110S.gb/3345929‑3345991
                                  |                             
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2319545/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:842477/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:809376/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:750366/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:617340/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:509440/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:351009/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:27447/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2487058/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2365751/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2347985/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1181362/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2257782/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:2065778/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1976883/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1966866/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1845610/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1837802/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1669241/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1641163/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1564627/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1431473/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTTATCCATTCCGCCGTCAAACCAGCGTa  >  1:1396948/1‑64 (MQ=255)
tggCACGCCGCACTGTTGCGAAGTACCGAGAGTC‑t                              >  1:624339/1‑35 (MQ=255)
                                  |                             
TGGCACGCCGCACTGTTGCGAAGTACCGAGAGTC‑TTTATCCATTCCGCCGTCAAACCAGCGTA  >  W3110S.gb/3345929‑3345991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A