Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,500,231 |
C→T |
A115T (GCG→ACG) |
yijE ← |
predicted permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,500,231 | 0 | C | T | 100.0%
| 89.0
/ NA
| 29 | A115T (GCG→ACG) | yijE | predicted permease |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/29); total (0/29) |
AGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCT > W3110S.gb/3500172‑3500267
|
aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa < 1:2208046/65‑1 (MQ=255)
aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa < 1:1156666/65‑1 (MQ=255)
aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa < 1:533748/65‑1 (MQ=255)
aGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa < 1:1896740/65‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1040038/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:259877/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:2539208/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:2175451/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:435226/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1986087/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:548001/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:680316/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:839904/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1587614/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1498050/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1320113/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1311733/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:846580/61‑1 (MQ=255)
ttGCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1080490/61‑1 (MQ=255)
gCCCACGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGc < 1:1803550/59‑1 (MQ=255)
aCGTCGCAGGCGTTCACCGAGAAACAACGTGGCGa < 1:1411788/35‑1 (MQ=255)
cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:1065883/65‑1 (MQ=255)
cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:339708/65‑1 (MQ=255)
cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:2170793/65‑1 (MQ=255)
cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:2152184/65‑1 (MQ=255)
cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:1694468/65‑1 (MQ=255)
cGTCGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:1154225/65‑1 (MQ=255)
cGCAGGCGTTCACCGAGAAACAACGTGGCGAAAATCACCACCCAGAACGGCATGGTATAGCt < 1:1871361/62‑1 (MQ=255)
ccGAGAAACAACGTGGAGAAAATCACCACCCAGAACGGc < 1:1911704/39‑1 (MQ=37)
|
AGCGGCAATCAGAATCGCGAAATATTGCCCACGTCGCAGGCGTTCACCGAGAAACAACGCGGCGAAAATCACCACCCAGAACGGCATGGTATAGCT > W3110S.gb/3500172‑3500267
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
