Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,509,602 |
Δ1 bp |
coding (67/213 nt) |
rpmE ← |
50S ribosomal subunit protein L31 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,509,599 | 0 | T | . | 100.0%
| 20.1
/ NA
| 6 | coding (70/213 nt) | rpmE | 50S ribosomal subunit protein L31 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (0/6); total (0/6) |
CAACGGTGGAGCGGATTTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCG > W3110S.gb/3509584‑3509649
|
cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:1508064/65‑1 (MQ=255)
cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:1991062/65‑1 (MQ=255)
cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:2314134/65‑1 (MQ=255)
cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:2363969/65‑1 (MQ=255)
cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:713925/65‑1 (MQ=255)
cAACGGTGGAGCGGA‑TTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCg < 1:735875/65‑1 (MQ=255)
|
CAACGGTGGAGCGGATTTTCATTACGTTACCGCAAGAGCAGCTAGCAGTAATTTCTTCGTATTTCG > W3110S.gb/3509584‑3509649
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
