Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,785,326 |
+C |
coding (1170/1185 nt) |
emrD ← |
multidrug efflux system protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,785,322 | 1 | . | C | 100.0%
| 20.2
/ NA
| 7 | Q392E (CAG→GAG) | emrD | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/7); total (0/7) |
GCGCTTAAACGGGCTG‑CCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAT > W3110S.gb/3785307‑3785370
|
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:1040820/65‑1 (MQ=255)
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:1105181/65‑1 (MQ=255)
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:1325787/65‑1 (MQ=255)
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:135929/65‑1 (MQ=255)
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:399686/65‑1 (MQ=255)
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:447678/65‑1 (MQ=255)
gcgcTTAAACGGGCTGCCCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAt < 1:78375/65‑1 (MQ=255)
|
GCGCTTAAACGGGCTG‑CCCCTGATGCGACATCCGCGTCGCCAGCGGCAGCCAGCACAGCACGAT > W3110S.gb/3785307‑3785370
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
