Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
711,918 |
+A |
intergenic (‑31/+109) |
fldA ← / ← ybfE |
flavodoxin 1/LexA regulated protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 711,915 | 1 | . | A | 100.0%
| 15.4
/ NA
| 6 | intergenic (‑28/+112) | fldA/ybfE | flavodoxin 1/LexA regulated protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/6); total (0/6) |
CTCTTGAAACTTATTG‑AAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGAA > W3110S.gb/711900‑711962
|
ctctTGAAACTTATTGAAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGaa < 1:1398952/64‑1 (MQ=255)
ctctTGAAACTTATTGAAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGaa < 1:1431072/64‑1 (MQ=255)
ctctTGAAACTTATTGAAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGaa < 1:1433836/64‑1 (MQ=255)
ctctTGAAACTTATTGAAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGaa < 1:318091/64‑1 (MQ=255)
ctctTGAAACTTATTGAAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGaa < 1:454589/64‑1 (MQ=255)
ctctTGAAACTTATTGAAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGaa < 1:48617/64‑1 (MQ=255)
|
CTCTTGAAACTTATTG‑AAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGAA > W3110S.gb/711900‑711962
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
