Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R1 178 99.0 1887089 95.3% 1798395 63.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,029,261 C→T A109A (GCG→GCA)  ygfT ← fused predicted oxidoreductase Fe‑S subunit and nucleotide‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,029,2610CT100.0% 469.9 / NA 127A109A (GCG→GCA) ygfTfused predicted oxidoreductase Fe‑S subunit and nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/127);  total (0/127)

Only 100 of 127 total aligned reads displayed.

CTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAG  >  W3110S.gb/3029237‑3029301
                        |                                        
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCTACGACGCCAAAGGGGCAAg  <  1:1354857/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGAGGCCAAAGGGGCAAg  <  1:1000661/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCTAg  <  1:244368/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCTAg  <  1:242130/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:322290/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:50124/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:450453/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:446600/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1013707/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:423943/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:372962/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:363653/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:35281/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:34578/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:335652/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1807945/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:274040/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1767917/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1805663/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:223413/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:221598/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:201688/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:192306/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1882330/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1844468/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1818556/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:816851/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:98192/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:98079/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:967503/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:948506/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:935458/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:919605/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:914089/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:901657/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:895671/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:895625/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:835038/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:545364/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:807140/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:774692/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:7520/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:746675/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:735569/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:69189/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:67389/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:650878/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:628273/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:571039/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1172228/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1744877/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:13858/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1373336/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1033843/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1366340/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1317745/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1283450/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1281430/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1256539/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1181612/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:11740/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1407909/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1141156/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1127054/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1120301/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1103355/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1086867/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1082197/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1070220/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1066149/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1064662/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1062779/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1057359/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1408071/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:171873/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1715152/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1707178/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1697130/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1665284/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1659571/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1651692/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1641254/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1626645/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1614375/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1592856/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1581788/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:158094/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1529955/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1521514/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1515634/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1511787/65‑1 (MQ=255)
cTGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1449002/65‑1 (MQ=255)
cTGGTTACAAAGGACGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1615999/65‑1 (MQ=255)
cTGATTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1445963/65‑1 (MQ=255)
 tGGTTACAAAGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1577776/64‑1 (MQ=255)
          aGGTCGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1757937/55‑1 (MQ=255)
              cGCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:818366/51‑1 (MQ=255)
               gCATTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:1030901/50‑1 (MQ=255)
                 aTTTCTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:517523/48‑1 (MQ=255)
                     cTGTGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAg  <  1:585425/44‑1 (MQ=255)
                        |                                        
CTGGTTACAAAGGTCGCATTTCTGCGCAATCGTATCGACCATCTCAACGACGCCAAAGGGGCAAG  >  W3110S.gb/3029237‑3029301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A