Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I3 R1 178 99.0 1887089 95.3% 1798395 63.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,383,928 C→T A66T (GCG→ACG)  mdh ← malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,383,9280CT100.0% 210.2 / NA 56A66T (GCG→ACG) mdhmalate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (56/0);  total (56/0)

GTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCT  >  W3110S.gb/3383877‑3383956
                                                   |                            
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGTCTTCCAGCGTCGGAGTCGCATc                  >  1:950516/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCAt                   >  1:433883/1‑63 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1035238/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:361314/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1596473/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1563104/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1361709/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1231433/1‑64 (MQ=255)
gTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATc                  >  1:1008794/1‑64 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTGGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1131467/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGc                     >  1:1692815/1‑46 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCAt                   >  1:1588145/1‑48 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCtt                >  1:310497/1‑51 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCt                 >  1:1669234/1‑50 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCCCCAGAAAAACCt  >  1:1222744/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAg          >  1:141551/1‑57 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAccc   >  1:39314/1‑64 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAAcc   >  1:1143740/1‑64 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAAcc   >  1:1780366/1‑64 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAAcc   >  1:901684/1‑64 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:870583/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:287231/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:290239/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1194728/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1088813/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1074963/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:466374/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:476000/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:755899/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:52651/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:538157/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:865673/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:561466/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:566629/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:602146/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:695574/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1562877/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1253559/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1167856/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1409532/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1451108/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1497153/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1539017/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1553732/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1554999/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:260264/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1608812/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1612530/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1827202/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1875779/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:1882048/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:23558/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCt  >  1:240561/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACAAGAAAAACCt  >  1:933830/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATATTCACCAGAAAAACCt  >  1:217765/1‑65 (MQ=255)
               cTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGCAGTCGCATCTTCACCAGAAAAACCt  >  1:175481/1‑65 (MQ=255)
                                                   |                            
GTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCT  >  W3110S.gb/3383877‑3383956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A